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mmu_circDiaph2_1062
Basic information of circular RNA
PanCircBase ID:
mmu_circDiaph2_1062
mm39_Chr. Position ID:
chrX|128803648|128863733|+
Gene name:
Diaph2
Transcript:
ENSMUST00000127577.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
5,6,7,8,9,10,11,12,13,14
Exon/intron offsets:
0,36345,36503,39999,40943,49773,52994,54343,57144,60020
Genomic length:
60085
Exon/intron sizes in nt:
140,75,70,137,109,111,119,117,119,65
Flanking exon/introns:
chrX:128726696-128803648|chrX:128863733-128867274
Splice length:
1062
Other database IDs:
circAtlas: mmu-Diap2_0010
circAtlas: hsa-DIAPH2_0018
Human Ortholog IDs:
Mature spliced Sequence:
GGTGGCCTGAAAAACAGCAAACATGAGTTCACCCTTTCTTCACAAGAATATGTTCATGAATTACGATCTGGCATCTCAGATGAGAAACTTCTCAACTGCCTGGAATCTTTGAGAGTGTCTTTAACCAGCCATCCTGTCAGCTGGGTCAACAACTTTGGCTATGAAGGACTTGGAGTCTTACTGGATGTGCTGGAGAAGCTTCTGGACAAAAAACAGCAAGAAAATATCGACAAGAAGAATCAGTACAAAGTCATTCAGTGCCTGAAAGCATTTATGAATAATAAGTTTGGACTACAAAGAATTCTAGGAGACGAGAGAAGCCTTTTACTGTTGGCAAGAGCAATTGACCCGAAACAACAGAATATGATGACTGAAATAGTAAAAATCCTTTCTGCTATTTGCATTGTTGGGGAAGAAAACATTCTAGACAAACTTTTAGGGGGCATCACTGCTGCAGCTGAACTAAATAATAGAGAACGATTTTCACCAATTGTGGAAGGCTTAGAAAATAATGAAGCCTTGCATCTCCAGGTGGCCTGCATGCAGTTCATAAATGCACTTGTCACCTCTCCATATGATCTTGATTTTCGAATTCATTTGAGGAATGAATTCCTTCGTTGTGGATTAAAAGCAATGCTCCCTACTCTTAAAGAGATAGAGAATGAAGGGCTTGATATTCAGTTGAGAGTGTTTGAAGAGAACAAAGAAGATGACCTAAGTGAATTATCCCACCGTCTCAATGACATTCGAGCTGAAATGGATGATATTAATGAAGTCTACCATCTGCTGTATAATATGCTGAAGGACACTGCTGCGGAACCTTACTTATTATCCATTCTCCAACACTTTCTGCTCATCAGAAATGATTACTACATCAGGCCACAGTATTATAAAATCATTGAGGAGTGTGTTTCACAGATAGTGCTACACTGCAGTGGCATGGACCCAGACTTCAAGTACCGGCAGCGCATAGACTTTGATTTCACTCATCTCCTAGATGCTTGTGTAAATAAGGCAAAAGTTGAAGAAAATGAGCAAAAAGCAATGGAATTTTCAAAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGCGCATAGACTTTGATT
56.88
TGTGAAGAAAGGGTGAACTCA
57.08
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGCGCATAGACTTTGATTTCA
60.18
TGTGAAGAAAGGGTGAACTCA
57.08
144
PCR template sequence:
GCAGCGCATAGACTTTGATTTCACTCATCTCCTAGATGCTTGTGTAAATAAGGCAAAAGTTGAAGAAAATGAGCAAAAAGCAATGGAATTTTCAAAGAAGGGTGGCCTGAAAAACAGCAAACATGAGTTCACCCTTTCTTCACAAGAATATGTTCATGAATTACGATCTGGCATCTCAGATGAGAAACTTCTCAACTGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-200c-3p(1), mmu-miR-205-5p(1), mmu-miR-217-5p(1), mmu-miR-382-5p(1), mmu-miR-499-5p(1), mmu-miR-207(1), mmu-miR-369-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTTTCAAAGAAGGGTGGCCTGAAAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDIAPH2_1062_ORF_1
1.063
0.146665615
0.999976089
1851
ORF sequence:
ATGAATAATAAGTTTGGACTACAAAGAATTCTAGGAGACGAGAGAAGCCTTTTACTGTTGGCAAGAGCAATTGACCCGAAACAACAGAATATGATGACTGAAATAGTAAAAATCCTTTCTGCTATTTGCATTGTTGGGGAAGAAAACATTCTAGACAAACTTTTAGGGGGCATCACTGCTGCAGCTGAACTAAATAATAGAGAACGATTTTCACCAATTGTGGAAGGCTTAGAAAATAATGAAGCCTTGCATCTCCAGGTGGCCTGCATGCAGTTCATAAATGCACTTGTCACCTCTCCATATGATCTTGATTTTCGAATTCATTTGAGGAATGAATTCCTTCGTTGTGGATTAAAAGCAATGCTCCCTACTCTTAAAGAGATAGAGAATGAAGGGCTTGATATTCAGTTGAGAGTGTTTGAAGAGAACAAAGAAGATGACCTAAGTGAATTATCCCACCGTCTCAATGACATTCGAGCTGAAATGGATGATATTAATGAAGTCTACCATCTGCTGTATAATATGCTGAAGGACACTGCTGCGGAACCTTACTTATTATCCATTCTCCAACACTTTCTGCTCATCAGAAATGATTACTACATCAGGCCACAGTATTATAAAATCATTGAGGAGTGTGTTTCACAGATAGTGCTACACTGCAGTGGCATGGACCCAGACTTCAAGTACCGGCAGCGCATAGACTTTGATTTCACTCATCTCCTAGATGCTTGTGTAAATAAGGCAAAAGTTGAAGAAAATGAGCAAAAAGCAATGGAATTTTCAAAGAAGGGTGGCCTGAAAAACAGCAAACATGAGTTCACCCTTTCTTCACAAGAATATGTTCATGAATTACGATCTGGCATCTCAGATGAGAAACTTCTCAACTGCCTGGAATCTTTGAGAGTGTCTTTAACCAGCCATCCTGTCAGCTGGGTCAACAACTTTGGCTATGAAGGACTTGGAGTCTTACTGGATGTGCTGGAGAAGCTTCTGGACAAAAAACAGCAAGAAAATATCGACAAGAAGAATCAGTACAAAGTCATTCAGTGCCTGAAAGCATTTATGAATAATAAGTTTGGACTACAAAGAATTCTAGGAGACGAGAGAAGCCTTTTACTGTTGGCAAGAGCAATTGACCCGAAACAACAGAATATGATGACTGAAATAGTAAAAATCCTTTCTGCTATTTGCATTGTTGGGGAAGAAAACATTCTAGACAAACTTTTAGGGGGCATCACTGCTGCAGCTGAACTAAATAATAGAGAACGATTTTCACCAATTGTGGAAGGCTTAGAAAATAATGAAGCCTTGCATCTCCAGGTGGCCTGCATGCAGTTCATAAATGCACTTGTCACCTCTCCATATGATCTTGATTTTCGAATTCATTTGAGGAATGAATTCCTTCGTTGTGGATTAAAAGCAATGCTCCCTACTCTTAAAGAGATAGAGAATGAAGGGCTTGATATTCAGTTGAGAGTGTTTGAAGAGAACAAAGAAGATGACCTAAGTGAATTATCCCACCGTCTCAATGACATTCGAGCTGAAATGGATGATATTAATGAAGTCTACCATCTGCTGTATAATATGCTGAAGGACACTGCTGCGGAACCTTACTTATTATCCATTCTCCAACACTTTCTGCTCATCAGAAATGATTACTACATCAGGCCACAGTATTATAAAATCATTGAGGAGTGTGTTTCACAGATAGTGCTACACTGCAGTGGCATGGACCCAGACTTCAAGTACCGGCAGCGCATAGACTTTGATTTCACTCATCTCCTAGATGCTTGTGTAAATAAGGCAAAAGTTGAAGAAAATGAGCAAAAAGCAATGGAATTTTCAAAGAAG
Peptide sequence:
MNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKKGGLKNSKHEFTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSHPVSWVNNFGYEGLGVLLDVLEKLLDKKQQENIDKKNQYKVIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQQNMMTEIVKILSAICIVGEENILDKLLGGITAAAELNNRERFSPIVEGLENNEALHLQVACMQFINALVTSPYDLDFRIHLRNEFLRCGLKAMLPTLKEIENEGLDIQLRVFEENKEDDLSELSHRLNDIRAEMDDINEVYHLLYNMLKDTAAEPYLLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRIDFDFTHLLDACVNKAKVEENEQKAMEFSKK
IRESfinder result
IRESfinder score
0.692125702
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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