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mmu_circDars2_901
Basic information of circular RNA
PanCircBase ID:
mmu_circDars2_901
mm39_Chr. Position ID:
chr1|160878900|160892861|-
Gene name:
Dars2
Transcript:
ENSMUST00000035430.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
11,10,9,8,7,6,5,4,3
Exon/intron offsets:
0,2610,5130,6116,8714,9405,11451,11916,13894
Genomic length:
13961
Exon/intron sizes in nt:
108,180,70,107,47,124,96,102,67
Flanking exon/introns:
chr1:160877570-160878900|chr1:160892861-160896649
Splice length:
901
Other database IDs:
circAtlas: mmu-Dars2_0009
Human Ortholog IDs:
Mature spliced Sequence:
GCAGAACACCTTCTTAGTCCTAAGAGATTGCCACGGCCTTGTTCAGATTCTCATTCCCCAGGATGAGTCAGCCGCATCTGTGAGGAGGATTTTGTGTGAAGCCCCTGTGGAGTCTGTGGTGAGAGTATCTGGGACAGTAATCTCCCGTCCTCCAGGACAAGAGAATCCGAAAATGCCGACGGGTGAGATTGAAATCAAAGTTAAAACAGCTGAGCTCCTGAATGCCTGCAAAAAGCTGCCCTTTGAGATTAAAGACTTTGTGAAGAAAACAGAGGCGCTCCGTTTGCAGTATCGCTACTTAGATCTGCGCAGCTTCCAGATGCAGTACAACTTGCGGCTGAGGTCGCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCACGGGTTTGTGGATATAGAAACCCCAACCTTGTTTAAGAGGACCCCTGGAGGTGCCAAGGAGTTTTTAGTACCATCCAGGGAACCTGGAAAGTTTTATTCCCTCCCTCAAAGTCCTCAGCAATTTAAGCAGCTTCTGATGGTTGGAGGTTTAGACAGGTATTTTCAGGTCGCTCGGTGTTACCGAGATGAAGGCTCAAGACCGGATAGACAGCCAGAGTTCACTCAGATTGACATAGAGATGTCCTTTGTAGAGCAGACTGGCATTCAGCGTTTAGTTGAGGGTTTGCTGCAGTATTCCTGGCCTGGTGATAAAGACCCTCTAGTGACTCCTTTCCCTTCCATGACTTTTGCTGAGGCACTGGCCACCTATGGAACTGATAAGCCAGATACTCGCTTTGGGATGAAGATTGTGGATGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACGGAGCG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGGATGCACTGGCTAAGC
60.11
ACAGATGCGGCTGACTCATC
60.18
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTTCAGGATGCACTGGC
60.32
ACAGATGCGGCTGACTCATC
60.18
147
PCR template sequence:
TGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACGGAGCGGCAGAACACCTTCTTAGTCCTAAGAGATTGCCACGGCCTTGTTCAGATTCTCATTCCCCAGGATGAGTCAGCCGCATCTGTGAGGAGGATTTTGTGTGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-216a-5p(2), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-217-5p(1), mmu-miR-362-5p(1), mmu-miR-27a-5p(1), mmu-miR-377-3p(1), mmu-miR-350-3p(1), mmu-miR-339-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCATGACGGAGCGGCAGAACACCTTC
siRNA sets
GAAGGTGTTCTGCCGCTCC, TTCTGCCGCTCCGTCATGA, GTTCTGCCGCTCCGTCATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCDARS2_901_ORF_1
1.0241
0.175944157
0.989741689
1002
ORF sequence:
ATGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACGGAGCGGCAGAACACCTTCTTAGTCCTAAGAGATTGCCACGGCCTTGTTCAGATTCTCATTCCCCAGGATGAGTCAGCCGCATCTGTGAGGAGGATTTTGTGTGAAGCCCCTGTGGAGTCTGTGGTGAGAGTATCTGGGACAGTAATCTCCCGTCCTCCAGGACAAGAGAATCCGAAAATGCCGACGGGTGAGATTGAAATCAAAGTTAAAACAGCTGAGCTCCTGAATGCCTGCAAAAAGCTGCCCTTTGAGATTAAAGACTTTGTGAAGAAAACAGAGGCGCTCCGTTTGCAGTATCGCTACTTAGATCTGCGCAGCTTCCAGATGCAGTACAACTTGCGGCTGAGGTCGCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCACGGGTTTGTGGATATAGAAACCCCAACCTTGTTTAAGAGGACCCCTGGAGGTGCCAAGGAGTTTTTAGTACCATCCAGGGAACCTGGAAAGTTTTATTCCCTCCCTCAAAGTCCTCAGCAATTTAAGCAGCTTCTGATGGTTGGAGGTTTAGACAGGTATTTTCAGGTCGCTCGGTGTTACCGAGATGAAGGCTCAAGACCGGATAGACAGCCAGAGTTCACTCAGATTGACATAGAGATGTCCTTTGTAGAGCAGACTGGCATTCAGCGTTTAGTTGAGGGTTTGCTGCAGTATTCCTGGCCTGGTGATAAAGACCCTCTAGTGACTCCTTTCCCTTCCATGACTTTTGCTGAGGCACTGGCCACCTATGGAACTGATAAGCCAGATACTCGCTTTGGGATGAAGATTGTGGATGTCAGTGATGTGTTCAGAAACACAGAGCTCAGATTCCTTCAGGATGCACTGGCTAAGCCCCAAGGAACTGTCAAAGCAATATGTGTTCATGACGGAGCG
Peptide sequence:
MSVMCSETQSSDSFRMHWLSPKELSKQYVFMTERQNTFLVLRDCHGLVQILIPQDESAASVRRILCEAPVESVVRVSGTVISRPPGQENPKMPTGEIEIKVKTAELLNACKKLPFEIKDFVKKTEALRLQYRYLDLRSFQMQYNLRLRSQMVMKMREYLCNLHGFVDIETPTLFKRTPGGAKEFLVPSREPGKFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPEFTQIDIEMSFVEQTGIQRLVEGLLQYSWPGDKDPLVTPFPSMTFAEALATYGTDKPDTRFGMKIVDVSDVFRNTELRFLQDALAKPQGTVKAICVHDGA
IRESfinder result
IRESfinder score
0.550154817
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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