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mmu_circCrebl2_380
Basic information of circular RNA
PanCircBase ID:
mmu_circCrebl2_380
mm39_Chr. Position ID:
chr6|134826065|134829277|+
Gene name:
Crebl2
Transcript:
ENSMUST00000046303.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,1977,3175
Genomic length:
3212
Exon/intron sizes in nt:
198,145,37
Flanking exon/introns:
chr6:134807407-134826065|chr6:134829277-134832936
Splice length:
380
Other database IDs:
circAtlas: mmu-Crebl2_0001
Human Ortholog IDs:
Mature spliced Sequence:
GTGGTTGGAGGCAAAGTGAAGAAGCCTGGTAAGCGAGGACGGAAGCCAGCCAAAATCGACTTGAAAGCGAAACTTGAGCGAAGCCGGCAGAGTGCGAGAGAATGCCGGGCCAGGAAAAAGCTGAGGTACCAGTACTTGGAGGAGTTGGTATCCAGTCGGGAAAGAGCTATATGTGCACTCCGAGAGGAACTGGAAATGTACAAGCAGTGGTGCATGGCAATGGACCAAGGGAAAATCCCTTCTGAAATAAGGGCCCTACTCACTGGCGAGGAGCAGAGCAAGCCTCAGCAGAGCTCAAGCAGGCATCCCAAAGCTGGGAAGACAGACGCTGACACCAACTCGTTGGTGGGGAATTGAACCTGTGGCTTCAGATACGATGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGACAGACGCTGACACCA
59.97
GCTTCGCTCAAGTTTCGCTT
59.76
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAGACGCTGACACCAACTC
60.25
GCTTCGCTCAAGTTTCGCTT
59.76
143
PCR template sequence:
AGCCTCAGCAGAGCTCAAGCAGGCATCCCAAAGCTGGGAAGACAGACGCTGACACCAACTCGTTGGTGGGGAATTGAACCTGTGGCTTCAGATACGATGAGTGGTTGGAGGCAAAGTGAAGAAGCCTGGTAAGCGAGGACGGAAGCCAGCCAAAATCGACTTGAAAGCGAAACTTGAGCGAAGCCGGCAGAGTGCGAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-541-5p(1), mmu-miR-3470b(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGATACGATGAGTGGTTGGAGGCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.242455842
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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