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mmu_circCnot1_663
Basic information of circular RNA
PanCircBase ID:
mmu_circCnot1_663
mm39_Chr. Position ID:
chr8|96466435|96468705|-
Gene name:
Cnot1
Transcript:
ENSMUST00000098473.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
34,33,32,31
Exon/intron offsets:
0,922,1121,1973
Genomic length:
2270
Exon/intron sizes in nt:
1,20,10,51,41,297
Flanking exon/introns:
chr8:96463073-96466435|chr8:96468705-96469731
Splice length:
663
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATCCCTTTATTTCAAGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTCCATCCTGTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTGCCCTGGATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAATGGCTATGATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCAGCCCTGCGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACAATTGTGAATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAGATTGGCAACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTCTTAACCTACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGCTAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTACAGGGTTTTTAGCTCAGCCCATGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CACGAAATGTGCCTGGCTTC
60.11
GGATGGACAAGCTCCTGGAC
60.11
157
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACGAAATGTGCCTGGCTTCT
60.25
GGATGGACAAGCTCCTGGAC
60.11
156
PCR template sequence:
GCAGCTAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTACAGGGTTTTTAGCTCAGCCCATGAAGATCCCTTTATTTCAAGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTCCATCCTGTGGTGGATCGCTCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-15b-5p(1), mmu-miR-19a-3p(1), mmu-miR-320-3p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-676-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGCCCATGAAGATCCCTTTATTTC
siRNA sets
ATAAAGGGATCTTCATGGG, AATAAAGGGATCTTCATGG, TAAAGGGATCTTCATGGGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCNOT1_663_ORF_1
1.1012
0.218354989
0.998397103
1215
ORF sequence:
ATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTGCCCTGGATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAATGGCTATGATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCAGCCCTGCGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACAATTGTGAATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAGATTGGCAACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTCTTAACCTACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGCTAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTACAGGGTTTTTAGCTCAGCCCATGAAGATCCCTTTATTTCAAGCTCATCCTCAGCTGAAGCAGTGTGTGCGGCAAGCAATTGAAAGAGCTGTCCAGGAGCTTGTCCATCCTGTGGTGGATCGCTCAATTAAGATTGCTATGACTACGTGCGAACAGATAGTCAGAAAGGACTTTGCCCTGGATTCTGAGGAGTCTCGAATGAGAATAGCAGCTCATCACATGATGCGGAACTTGACAGCTGGAATGGCTATGATTACTTGCAGGGAACCTTTACTGATGAGCATATCCACCAACCTCAAAAACAGCTTTGCCTCAGCCCTGCGTACTGCATCCCCACAGCAAAGGGAAATGATGGACCAGGCTGCTGCTCAGTTAGCTCAGGACAATTGTGAATTAGCTTGTTGTTTTATTCAGAAAACTGCAGTGGAGAAAGCAGGCCCTGAGATGGACAAGAGATTGGCAACTGAATTTGAGCTGAGGAAGCATGCCCGGCAAGAAGGACGTAGGTACTGTGATCCTGTGGTCTTAACCTACCAAGCCGAGCGGATGCCGGAGCAGATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGCTAGCCGTCTATGAAGAGTTTGCACGAAATGTGCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCTACAGGGTTTTTAGCTCAGCCCATGAAG
Peptide sequence:
MTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMKIPLFQAHPQLKQCVRQAIERAVQELVHPVVDRSIKIAMTTCEQIVRKDFALDSEESRMRIAAHHMMRNLTAGMAMITCREPLLMSISTNLKNSFASALRTASPQQREMMDQAAAQLAQDNCELACCFIQKTAVEKAGPEMDKRLATEFELRKHARQEGRRYCDPVVLTYQAERMPEQIRLKVGGVDPKQLAVYEEFARNVPGFLPTNDLSQPTGFLAQPMK
IRESfinder result
IRESfinder score
0.409568298
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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