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mmu_circCluap1_691
Basic information of circular RNA
PanCircBase ID:
mmu_circCluap1_691
mm39_Chr. Position ID:
chr16|3729314|3741837|+
Gene name:
Cluap1
Transcript:
ENSMUST00000040881.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
2,3,4,5,6,7
Exon/intron offsets:
0,1834,3972,6375,8030,12389
Genomic length:
12523
Exon/intron sizes in nt:
11,28,51,80,96,84,134
Flanking exon/introns:
chr16:3727017-3729314|chr16:3741837-3746353
Splice length:
691
Other database IDs:
CIRCpedia: MMU_CIRCpedia_19053, circAtlas: mmu-Cluap1_0001
circBase: hsa_circ_0037604, circAtlas: hsa-CLUAP1_0001, CIRCpedia: HSA_CIRCpedia_130066
Human Ortholog IDs:
Mature spliced Sequence:
ATTTCACCGAGATGATGAGAGCCTTGGGGTACCCACGCCATATTTCTATGGAGAATTTCCGCACACCCAACTTTGGGCTTGTCTCTGAAGTGCTTCTGTGGCTTGTGAAAAGGTATGAGCCGCAGACTGACATCCCTTCTGACATTGAGACTGAACAAGACAGAGTTTTCTTCATTAAGGCAATTGCCCAGTTCATGGCCACAAAGGCACATATAAAACTCAATACCAAGAAGCTCTATCAAGCTGACGGGTATGCAGTGAAAGAGCTGCTGAAGATCACATCTGTTCTCTATAATGCAATGAAGACCAAAGGGATGGAGGGCTCCAATGTAGGAGAGGAAGACATCAGCAAATTCAAGTTTGATCTCGGCTCCAAGATTGCAGATTTGAAAGCAGCCAGACAGCTTGCGTCTGAAATCACTGCCAAAGGAGCCTCTCTGTATGACTTGCTGGGCAAAGAGGTTGAGCTGAGGGAACTGAGGACAGAAGCCATTGCTAGACCTCTAGAGATCAATGAGACTGAAAAGGTGATGAGGATTGCAATCAAAGATCTTTTGGCCCAGGTTCAGAAGACTAAAGACCTGCTTAATAATGTGGCCTCTGATGAAGCAAACTTAGAAGCCAAAATTGAAAAGAGAAAATTAGAACTGGAAAGAAATCGGAAGCGACTGCAGACCCTGCAGAGTGTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGGCCTCTGATGAAGCAA
59.6
CAAAGTTGGGTGTGCGGAAA
59.54
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTGGCCTCTGATGAAGCAA
59.6
GGGTGTGCGGAAATTCTCCA
60.61
167
PCR template sequence:
ATGTGGCCTCTGATGAAGCAAACTTAGAAGCCAAAATTGAAAAGAGAAAATTAGAACTGGAAAGAAATCGGAAGCGACTGCAGACCCTGCAGAGTGTCAGATTTCACCGAGATGATGAGAGCCTTGGGGTACCCACGCCATATTTCTATGGAGAATTTCCGCACACCCAACTTTGGGCTTGTCTCTGAAGTGCTTCTGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-452-3p(2), mmu-miR-9-5p(1), mmu-miR-135a-5p(1), mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-34b-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
TGCAGAGTGTCAGATTTCACCGAGAT
siRNA sets
TGAAATCTGACACTCTGCA,GTGAAATCTGACACTCTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCLUAP1_691_ORF_1
1.107
0.258778052
0.967181274
768
ORF sequence:
ATGATGAGAGCCTTGGGGTACCCACGCCATATTTCTATGGAGAATTTCCGCACACCCAACTTTGGGCTTGTCTCTGAAGTGCTTCTGTGGCTTGTGAAAAGGTATGAGCCGCAGACTGACATCCCTTCTGACATTGAGACTGAACAAGACAGAGTTTTCTTCATTAAGGCAATTGCCCAGTTCATGGCCACAAAGGCACATATAAAACTCAATACCAAGAAGCTCTATCAAGCTGACGGGTATGCAGTGAAAGAGCTGCTGAAGATCACATCTGTTCTCTATAATGCAATGAAGACCAAAGGGATGGAGGGCTCCAATGTAGGAGAGGAAGACATCAGCAAATTCAAGTTTGATCTCGGCTCCAAGATTGCAGATTTGAAAGCAGCCAGACAGCTTGCGTCTGAAATCACTGCCAAAGGAGCCTCTCTGTATGACTTGCTGGGCAAAGAGGTTGAGCTGAGGGAACTGAGGACAGAAGCCATTGCTAGACCTCTAGAGATCAATGAGACTGAAAAGGTGATGAGGATTGCAATCAAAGATCTTTTGGCCCAGGTTCAGAAGACTAAAGACCTGCTTAATAATGTGGCCTCTGATGAAGCAAACTTAGAAGCCAAAATTGAAAAGAGAAAATTAGAACTGGAAAGAAATCGGAAGCGACTGCAGACCCTGCAGAGTGTCAGATTTCACCGAGATGATGAGAGCCTTGGGGTACCCACGCCATATTTCTATGGAGAATTTCCGCACACCCAACTTTGGGCTTGTCTCTGA
Peptide sequence:
MMRALGYPRHISMENFRTPNFGLVSEVLLWLVKRYEPQTDIPSDIETEQDRVFFIKAIAQFMATKAHIKLNTKKLYQADGYAVKELLKITSVLYNAMKTKGMEGSNVGEEDISKFKFDLGSKIADLKAARQLASEITAKGASLYDLLGKEVELRELRTEAIARPLEINETEKVMRIAIKDLLAQVQKTKDLLNNVASDEANLEAKIEKRKLELERNRKRLQTLQSVRFHRDDESLGVPTPYFYGEFPHTQLWACL
IRESfinder result
IRESfinder score
0.611501827
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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