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mmu_circCcdc186_469
Basic information of circular RNA
PanCircBase ID:
mmu_circCcdc186_469
mm39_Chr. Position ID:
chr19|56796409|56798797|-
Gene name:
Ccdc186
Transcript:
ENSMUST00000118592.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,1215,2261
Genomic length:
2388
Exon/intron sizes in nt:
21,31,29,127
Flanking exon/introns:
chr19:56795517-56796409|chr19:56798797-56801426
Splice length:
469
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTTAATTAAAGAAAATAAGAATCATCAGGAACTTATCTTAAACATTTGTTCAGAAAAAGACAGTTTAAGAGAAGAACTGAGAAAAAGGACAGAAACAGAGAAGCAGCATATGAACACAATAAAGCAGTTAGAATTAAGAATAGAGGAACTGAATAAAGAAATTAAAGCTTCCAAAGATCAGCTAGTCGCACAGGATGTCACAGCTAAAAATGCAATTCAGCAAATCCATAAAGAGATGGCCCAGCGGATGGACCAGGCCAATAAGAAATGTGAAGAGGCCCGTCAGGAAAAGGAGGCAATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAGAAACCCTTGAGAGGAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTCTCAGGAGAAAGGGCG
60.11
TCTGTTTCTGTCCTTTTTCTCAGT
58.25
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTCTCAGGAGAAAGGGCG
60.11
CTCTGTTTCTGTCCTTTTTCTCAGT
59.18
145
PCR template sequence:
GAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAGGTTAATTAAAGAAAATAAGAATCATCAGGAACTTATCTTAAACATTTGTTCAGAAAAAGACAGTTTAAGAGAAGAACTGAGAAAAAGGACAGAAACAGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
CTATGAATCGAAGGTTAATTAAAGAA
siRNA sets
TTAATTAACCTTCGATTCA, TTCTTTAATTAACCTTCGA, ATTAACCTTCGATTCATAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCCDC186_469_ORF_1
1.1124
0.147366784
0.807347734
558
ORF sequence:
ATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAGGTTAATTAAAGAAAATAAGAATCATCAGGAACTTATCTTAAACATTTGTTCAGAAAAAGACAGTTTAAGAGAAGAACTGAGAAAAAGGACAGAAACAGAGAAGCAGCATATGAACACAATAAAGCAGTTAGAATTAAGAATAGAGGAACTGAATAAAGAAATTAAAGCTTCCAAAGATCAGCTAGTCGCACAGGATGTCACAGCTAAAAATGCAATTCAGCAAATCCATAAAGAGATGGCCCAGCGGATGGACCAGGCCAATAAGAAATGTGAAGAGGCCCGTCAGGAAAAGGAGGCAATGGTAATGAAGTATGTGAGAGGCGAGAAGGAGGCTCTAGACCTGCGAAAGGAAAAAGAAACCCTTGAGAGGAAACTTAGAGATGCAAGTAAGGAATTGGAGAAAAACACTAACAAAATTAAGCAGCTGTCTCAGGAGAAAGGGCGGCTGCAGCAACTCTATGAATCGAAG
Peptide sequence:
MQVRNWRKTLTKLSSCLRRKGGCSNSMNRRLIKENKNHQELILNICSEKDSLREELRKRTETEKQHMNTIKQLELRIEELNKEIKASKDQLVAQDVTAKNAIQQIHKEMAQRMDQANKKCEEARQEKEAMVMKYVRGEKEALDLRKEKETLERKLRDASKELEKNTNKIKQLSQEKGRLQQLYESK
IRESfinder result
IRESfinder score
0.391106975
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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