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mmu_circCanx_1729
Basic information of circular RNA
PanCircBase ID:
mmu_circCanx_1729
mm39_Chr. Position ID:
chr11|50187884|50202611|-
Gene name:
Canx
Transcript:
ENSMUST00000020637.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
13
Exon/intron indexes:
14,13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,216,1059,2071,3840,4700,7268,10412,11252,11730,13314,13736,14546
Genomic length:
14727
Exon/intron sizes in nt:
80,12,11,20,21,61,57,10,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr11:50187162-50187884|chr11:50202611-50216319
Splice length:
1729
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATAGATCATGGAAGGGAAGTGGTTACTGTGTTTGCTGCTGGTCCTTGGAACTGCAGCTGTTGAGGCTCATGATGGACATGATGATGACGCGATTGATATTGAAGATGATCTTGATGATGTTATTGAAGAGGTAGAAGATTCAAAATCTAAATCAGATGCCAGCACTCCTCCATCTCCAAAGGTCACCTACAAAGCTCCAGTTCCAACAGGGGAGGTTTATTTTGCTGACTCCTTTGACAGAGGGTCTCTGTCAGGGTGGATTTTATCTAAAGCCAAAAAAGATGACACTGATGATGAAATTGCCAAATATGATGGAAAGTGGGAAGTAGATGAGATGAAGGAAACAAAGCTTCCAGGGGATAAAGGACTTGTACTGATGTCTCGGGCCAAGCATCATGCCATCTCTGCTAAACTGAATAAGCCCTTCCTGTTTGATACCAAGCCTCTCATTGTTCAGTATGAGGTTAATTTTCAGAATGGAATAGAATGTGGTGGTGCCTATGTGAAGCTGCTTTCCAAGACGGCAGAGCTCAGCCTGGATCAATTCCACGACAAGACTCCCTATACTATTATGTTTGGTCCAGATAAGTGTGGAGAGGACTACAAACTGCATTTCATCTTTCGACACAAAAATCCCAAGACAGGTGTATATGAAGAAAAACATGCTAAGAGGCCAGATGCAGATCTGAAGACCTATTTCACTGACAAGAAAACGCATCTTTATACATTAATCTTGAATCCAGACAATAGTTTTGAAATATTAGTTGACCAGTCTGTTGTGAACAGTGGAAATCTGCTAAATGACATGACTCCTCCTGTAAACCCTTCACGTGAAATTGAAGACCCAGAAGACCGGAAGCCTGAAGATTGGGATGAAAGGCCCAAAATAGCAGATCCAGATGCTGTCAAGCCAGATGACTGGGATGAAGACGCCCCTTCTAAGATCCCAGATGAAGAGGCCACCAAGCCTGAAGGCTGGCTAGACGACGAACCTGAGTATATTCCAGACCCTGATGCAGAGAAGCCAGAGGATTGGGATGAGGATATGGACGGAGAATGGGAGGCTCCTCAGATTGCCAACCCCAAGTGTGAGTCAGCCCCTGGGTGTGGTGTCTGGCAGCGACCTATGATTGACAACCCCAATTATAAGGGCAAATGGAAGCCTCCAATGATTGACAACCCTAACTACCAGGGAATCTGGAAACCAAGGAAAATACCAAATCCAGATTTCTTTGAAGACCTAGAACCTTTTAAGATGACTCCTTTCAGTGCTATTGGTTTGGAGCTCTGGTCCATGACATCCGACATCTTTTTTGACAACTTTATCATTAGTGGTGACCGAAGAGTAGTTGATGATTGGGCCAATGATGGGTGGGGCCTGAAGAAAGCTGCTGATGGGGCTGCTGAGCCAGGTGTAGTGCTGCAGATGCTGGAGGCAGCTGAAGAGCGTCCATGGCTTTGGGTGGTCTACATTTTGACTGTAGCTTTGCCAGTGTTCCTTGTGATCCTCTTCTGCTGTTCTGGAAAGAAACAGTCCAATGCTATGGAGTACAAGAAGACGGATGCTCCCCAGCCAGATGTGAAGGATGAAGAAGGGAAGGAAGAAGAGAAGAACAAGAGGGATGAAGAGGAAGAAGAGGAGAAGCTTGAAGAGAAACAGAAGAGTGATGCTGAAGAAGATGGTGTTACTGGCAGTCAAGATGAGGAAGATAGCAAGCCTAAAGCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTGTTACTGGCAGTCAAGA
59.23
TCGCGTCATCATCATGTCCA
59.54
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATGGTGTTACTGGCAGTCA
59.02
TCGCGTCATCATCATGTCCA
59.54
144
PCR template sequence:
GGAAGAAGAGGAGAAGCTTGAAGAGAAACAGAAGAGTGATGCTGAAGAAGATGGTGTTACTGGCAGTCAAGATGAGGAAGATAGCAAGCCTAAAGCAGAGATAGATCATGGAAGGGAAGTGGTTACTGTGTTTGCTGCTGGTCCTTGGAACTGCAGCTGTTGAGGCTCATGATGGACATGATGATGACGCGATTGATATT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-182-5p(2), mmu-miR-802-5p(2), mmu-miR-15a-5p(1), mmu-miR-103-3p(1), mmu-miR-205-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-15b-5p(1), mmu-miR-139-5p(1), mmu-miR-503-5p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-183-5p(1), mmu-miR-107-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCCTAAAGCAGAGATAGATCATGGAA
siRNA sets
TTCCATGATCTATCTCTGC, GATCTATCTCTGCTTTAGG, TCCATGATCTATCTCTGCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCCANX_1729_ORF_1
1.1375
0.293265294
0.999981011
1785
ORF sequence:
ATGGAAGGGAAGTGGTTACTGTGTTTGCTGCTGGTCCTTGGAACTGCAGCTGTTGAGGCTCATGATGGACATGATGATGACGCGATTGATATTGAAGATGATCTTGATGATGTTATTGAAGAGGTAGAAGATTCAAAATCTAAATCAGATGCCAGCACTCCTCCATCTCCAAAGGTCACCTACAAAGCTCCAGTTCCAACAGGGGAGGTTTATTTTGCTGACTCCTTTGACAGAGGGTCTCTGTCAGGGTGGATTTTATCTAAAGCCAAAAAAGATGACACTGATGATGAAATTGCCAAATATGATGGAAAGTGGGAAGTAGATGAGATGAAGGAAACAAAGCTTCCAGGGGATAAAGGACTTGTACTGATGTCTCGGGCCAAGCATCATGCCATCTCTGCTAAACTGAATAAGCCCTTCCTGTTTGATACCAAGCCTCTCATTGTTCAGTATGAGGTTAATTTTCAGAATGGAATAGAATGTGGTGGTGCCTATGTGAAGCTGCTTTCCAAGACGGCAGAGCTCAGCCTGGATCAATTCCACGACAAGACTCCCTATACTATTATGTTTGGTCCAGATAAGTGTGGAGAGGACTACAAACTGCATTTCATCTTTCGACACAAAAATCCCAAGACAGGTGTATATGAAGAAAAACATGCTAAGAGGCCAGATGCAGATCTGAAGACCTATTTCACTGACAAGAAAACGCATCTTTATACATTAATCTTGAATCCAGACAATAGTTTTGAAATATTAGTTGACCAGTCTGTTGTGAACAGTGGAAATCTGCTAAATGACATGACTCCTCCTGTAAACCCTTCACGTGAAATTGAAGACCCAGAAGACCGGAAGCCTGAAGATTGGGATGAAAGGCCCAAAATAGCAGATCCAGATGCTGTCAAGCCAGATGACTGGGATGAAGACGCCCCTTCTAAGATCCCAGATGAAGAGGCCACCAAGCCTGAAGGCTGGCTAGACGACGAACCTGAGTATATTCCAGACCCTGATGCAGAGAAGCCAGAGGATTGGGATGAGGATATGGACGGAGAATGGGAGGCTCCTCAGATTGCCAACCCCAAGTGTGAGTCAGCCCCTGGGTGTGGTGTCTGGCAGCGACCTATGATTGACAACCCCAATTATAAGGGCAAATGGAAGCCTCCAATGATTGACAACCCTAACTACCAGGGAATCTGGAAACCAAGGAAAATACCAAATCCAGATTTCTTTGAAGACCTAGAACCTTTTAAGATGACTCCTTTCAGTGCTATTGGTTTGGAGCTCTGGTCCATGACATCCGACATCTTTTTTGACAACTTTATCATTAGTGGTGACCGAAGAGTAGTTGATGATTGGGCCAATGATGGGTGGGGCCTGAAGAAAGCTGCTGATGGGGCTGCTGAGCCAGGTGTAGTGCTGCAGATGCTGGAGGCAGCTGAAGAGCGTCCATGGCTTTGGGTGGTCTACATTTTGACTGTAGCTTTGCCAGTGTTCCTTGTGATCCTCTTCTGCTGTTCTGGAAAGAAACAGTCCAATGCTATGGAGTACAAGAAGACGGATGCTCCCCAGCCAGATGTGAAGGATGAAGAAGGGAAGGAAGAAGAGAAGAACAAGAGGGATGAAGAGGAAGAAGAGGAGAAGCTTGAAGAGAAACAGAAGAGTGATGCTGAAGAAGATGGTGTTACTGGCAGTCAAGATGAGGAAGATAGCAAGCCTAAAGCAGAGATAGATCATGGAAGGGAAGTGGTTACTGTGTTTGCTGCTGGTCCTTGGAACTGCAGCTGTTGA
Peptide sequence:
MEGKWLLCLLLVLGTAAVEAHDGHDDDAIDIEDDLDDVIEEVEDSKSKSDASTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKETKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTAELSLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDADLKTYFTDKKTHLYTLILNPDNSFEILVDQSVVNSGNLLNDMTPPVNPSREIEDPEDRKPEDWDERPKIADPDAVKPDDWDEDAPSKIPDEEATKPEGWLDDEPEYIPDPDAEKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPMIDNPNYKGKWKPPMIDNPNYQGIWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVLQMLEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQSNAMEYKKTDAPQPDVKDEEGKEEEKNKRDEEEEEEKLEEKQKSDAEEDGVTGSQDEEDSKPKAEIDHGREVVTVFAAGPWNCSC
IRESfinder result
IRESfinder score
0.425078793
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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