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mmu_circBtbd7_446
Basic information of circular RNA
PanCircBase ID:
mmu_circBtbd7_446
mm39_Chr. Position ID:
chr12|102774158|102779102|-
Gene name:
Btbd7
Transcript:
ENSMUST00000223554.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
7,6,5
Exon/intron offsets:
0,3220,4735
Genomic length:
4944
Exon/intron sizes in nt:
161,76,209
Flanking exon/introns:
chr12:102761575-102774158|chr12:102779102-102803876
Splice length:
446
Other database IDs:
circBase: mmu_circ_0003674, CIRCpedia: MMU_CIRCpedia_775, circAtlas: mmu-Btbd7_0002
circBase: hsa_circ_0102943, circAtlas: hsa-BTBD7_0002, CIRCpedia: HSA_CIRCpedia_13502
Human Ortholog IDs:
Mature spliced Sequence:
GCTGTGAGGACATCATTGCTGAGAGCATCTCATTAGACACCTTAATTGCCGTCCTCAAGTGGAGCTCGCATCCATACGGCTCTAAGTGGGTGCACCGACAAGCTGTGCATTTCCTGTGTGAGGAGCTATCCCAGGTCATGACTTCGGATGTTTTTTATGAACTGAGCAAAGACCATCTGCTTACTGCTATCCAGTCTGACTACCTACAGGCAAGCGAACAAGATATCCTTAAATATCTGATTAAATGGGGTGAGCATCAGTTGATGAAAAGAATAGCAGACAGAGAGCCAAACTTACTGAGTGGCACTGCACATAGTGTGAACAAAAGAGGTGTGAAGAGAAGAGACCTGGACATAGAAGAACTTAGAGAGATCCTTTCTTCTCTCTTGCCTTTTGTGCGGATTGAACACATCCTACCTATAAACAGTGAGGTCTTAAGTGATGCA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCTTGCCTTTTGTGCGGA
60.18
GTCGGTGCACCCACTTAGAG
60.39
163
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCTTGCCTTTTGTGCGGA
60.18
GTATGGATGCGAGCTCCACT
59.61
141
PCR template sequence:
CCTGGACATAGAAGAACTTAGAGAGATCCTTTCTTCTCTCTTGCCTTTTGTGCGGATTGAACACATCCTACCTATAAACAGTGAGGTCTTAAGTGATGCAGCTGTGAGGACATCATTGCTGAGAGCATCTCATTAGACACCTTAATTGCCGTCCTCAAGTGGAGCTCGCATCCATACGGCTCTAAGTGGGTGCACCGACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-miR-375-3p(1), mmu-let-7c-5p(1), mmu-let-7d-5p(1), mmu-miR-24-3p(1), mmu-miR-342-3p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTAAGTGATGCAGCTGTGAGGACAT
siRNA sets
ATGTCCTCACAGCTGCATC, TGTCCTCACAGCTGCATCA, GTCCTCACAGCTGCATCAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTBD7_446_ORF_1
0.9555
0.027282354
0.291957773
345
ORF sequence:
ATGACTTCGGATGTTTTTTATGAACTGAGCAAAGACCATCTGCTTACTGCTATCCAGTCTGACTACCTACAGGCAAGCGAACAAGATATCCTTAAATATCTGATTAAATGGGGTGAGCATCAGTTGATGAAAAGAATAGCAGACAGAGAGCCAAACTTACTGAGTGGCACTGCACATAGTGTGAACAAAAGAGGTGTGAAGAGAAGAGACCTGGACATAGAAGAACTTAGAGAGATCCTTTCTTCTCTCTTGCCTTTTGTGCGGATTGAACACATCCTACCTATAAACAGTGAGGTCTTAAGTGATGCAGCTGTGAGGACATCATTGCTGAGAGCATCTCATTAG
Peptide sequence:
MTSDVFYELSKDHLLTAIQSDYLQASEQDILKYLIKWGEHQLMKRIADREPNLLSGTAHSVNKRGVKRRDLDIEELREILSSLLPFVRIEHILPINSEVLSDAAVRTSLLRASH
IRESfinder result
IRESfinder score
0.638330604
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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