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mmu_circBtaf1_423
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_423
mm39_Chr. Position ID:
chr19|36926467|36933934|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
3,4,5
Exon/intron offsets:
0,1966,7303
Genomic length:
7467
Exon/intron sizes in nt:
11,51,44,164
Flanking exon/introns:
chr19:36922735-36926467|chr19:36933934-36935772
Splice length:
423
Other database IDs:
circAtlas: mmu-Btaf1_0027
circBase: hsa_circ_0094415, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_129158
Human Ortholog IDs:
Mature spliced Sequence:
GTATTAATATATTTAAGGAGTACAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAACGTGCCTGAGTGGAATCCAGTGCCAAGAACCAAACAAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGAGAAGCAATTGGAATGAGT
60.25
GCTTCAACAGCTTGTCCTGC
60.04
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTATACCCCAACTTCAGCAGCC
60.36
GCACTGGATTCCACTCAGGC
61.03
140
PCR template sequence:
TAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTGTATTAATATATTTAAGGAGTACAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAACGTGCCTGAGTGGAATCCAGTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-5101(2), mmu-miR-335-3p(2)
siRNAs for circular RNA silencing
Junction sequence
AAACAAACAATCTGTATTAATATATT
siRNA sets
TATTAATACAGATTGTTTG, ATTAATACAGATTGTTTGT, AATATATTAATACAGATTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_423_ORF_1
1.0176
0.046399394
0.877788863
714
ORF sequence:
ATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTGTATTAATATATTTAAGGAGTACAAATTGGGATACCCGGATTGCAGCAGGACAAGCTGTTGAAGCTATAGTGAAAAACGTGCCTGAGTGGAATCCAGTGCCAAGAACCAAACAAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCT
Peptide sequence:
MEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSVLIYLRSTNWDTRIAAGQAVEAIVKNVPEWNPVPRTKQEPTESSMEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQS
IRESfinder result
IRESfinder score
0.674176064
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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