top of page

mmu_circBtaf1_385

Basic information of circular RNA
PanCircBase ID:

mmu_circBtaf1_385

mm39_Chr. Position ID:

chr19|36939038|36943987|+

Gene name:

Btaf1

Transcript:

ENSMUST00000099494.4

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

4

Exon/intron indexes:

7,8,9,10

Exon/intron offsets:

0,792,3979,4853

Genomic length:

4949

Exon/intron sizes in nt:

130,69,90,96

Flanking exon/introns:

chr19:36935909-36939038|chr19:36943987-36944075

Splice length:

385

Other database IDs:

circAtlas: mmu-Btaf1_0047

CIRCpedia: HSA_CIRCpedia_364502

Human Ortholog IDs:
Mature spliced Sequence:

CAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAA

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCAGCATCAAGAGTGGTTGG

59.48

TGGAATCACTTGCAGTCTGA

56.77

171

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TCAGCAGCATCAAGAGTGGT

59.31

TGGAATCACTTGCAGTCTGA

56.77

174

PCR template sequence:

GGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAACAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAAT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

4

Interacting miRNAs (#binding sites)

mmu-miR-93-5p(1), mmu-miR-106b-5p(1), mmu-miR-541-5p(1), mmu-miR-1199-5p(1)

siRNAs for circular RNA silencing
Junction sequence

CGTTTCTGATGAACAATGACAGCACT

siRNA sets

AGTGCTGTCATTGTTCATC, TCATTGTTCATCAGAAACG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBTAF1_385_ORF_1

0.9816

0.046972948

0.394301869

390

ORF sequence:

ATGAACAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAA

Peptide sequence:

MNNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDE

IRESfinder result

IRESfinder score

0.628629486

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

bottom of page