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mmu_circBtaf1_1273
Basic information of circular RNA
PanCircBase ID:
mmu_circBtaf1_1273
mm39_Chr. Position ID:
chr19|36928433|36947438|+
Gene name:
Btaf1
Transcript:
ENSMUST00000099494.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
4,5,6,7,8,9,10,11,12,13
Exon/intron offsets:
0,5337,7339,10605,11397,14584,15458,15642,18022,18880
Genomic length:
19005
Exon/intron sizes in nt:
144,164,137,130,69,90,96,177,141,125
Flanking exon/introns:
chr19:36926582-36928433|chr19:36947438-36950297
Splice length:
1273
Other database IDs:
CIRCpedia: MMU_CIRCpedia_18334, circAtlas: mmu-Btaf1_0065
Human Ortholog IDs:
Mature spliced Sequence:
AACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGACTCTCCTTTCATCCATGT
56.35
AAAGTGATGCCCCATGCTGT
60.25
148
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGACTCTCCTTTCATCCATGT
56.35
TGATGCCCCATGCTGTAACA
59.67
144
PCR template sequence:
GGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15a-5p(1), mmu-miR-93-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-106b-5p(1), mmu-miR-217-5p(1), mmu-miR-324-5p(1), mmu-miR-127-5p(1), mmu-miR-218-5p(1), mmu-miR-541-5p(1), mmu-miR-499-5p(1), mmu-miR-5101(1), mmu-miR-369-3p(1), mmu-miR-335-3p(1), mmu-miR-1199-5p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
TCCAGCAATGCAGAACCTACAGAGAG
siRNA sets
TAGGTTCTGCATTGCTGGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBTAF1_1273_ORF_1
0.9893
0.023893488
0.997751945
1311
ORF sequence:
ATGGAAGATTCATCTACTACAGATCGATTAAATTTTGACAGATTTGATATATGCAGATTGTTACAGCATGGGGCATCACTTTTGGGGTCTGCTGGTGCAGAATTTGAAGTCCAAGATGAAAAATCAGGTGAAGTGGATCCTAAAGAGAGGATAGCACGCCAACGGAAATTATTACAGAAGAAACTTGGACTTAATATGGGAGAAGCAATTGGAATGAGTACTGAAGAACTCTTCAACGATGAAGATTTGGATTATACCCCAACTTCAGCAGCCCTTGTAAACAAACAATCTACTCTCCAAGCAGCTGAATTGATTGATTCAGAATTCCGAGCAGGAATGAGTAATAGACAGAAGAACAAAGCTAAAAGAATGGCCAAGTTATTTGCAAAACAGAGATCCAGGGATGCAGTGGAAACGAATGAGAAGAGCAATGACAGCACTGATGGAGAGCCAGAAGAAAAGAGACGGAAAGTAGCAAATGTTGTTATTAATCAGACTGCAAGTGATTCCAAAGTCTTGATAGATAATGTTCCAGAAAGTTCTTCCTTAATTGAAGAGACAAATGAATGGCCTTTGGAAAGCTTTTGTGAAGAACTTTGCAATGACCTTTTTAATCCCTCCTGGGAGGTTCGACATGGGGCAGGTACTGGACTCAGAGAAATTCTTAAAGCTCATGGGAAAAGTGGTGGTAAAATGGGAGACAGCACTTTAGAGGAGATGATTCAGCAGCATCAAGAGTGGTTGGAAGACTTAGTCATTAGACTCCTTTGTGTTTTTGCGTTGGACAGATTTGGAGACTTCGTTTCTGATGAAGTTGTAGCGCCAGTTCGTGAGACTTGTGCTCAAACGTTAGGTGTGGTTTTAAAGCACATGAACGAAACAGGAGTTCATAAGACAGTGGATGTGCTGCTGAAATTATTGACACAAGAACAGTGGGAAGTTAGACATGGTGGTCTTCTGGGAATAAAATATGCTTTGGCAGTTCGTCAGGATGTAATTAATACTTTATTGCCTAAAGTCTTAACTCGAATAATCGAAGGACTCCAGGACCTTGATGACGATGTTAGAGCTGTTGCTGCAGCATCCCTGGTTCCTGTAGTAGAAAGCCTTGTCTACCTTCAGACACAAAAGGTACCCTCCATTATAAATACCTTATGGGATTCTCTTCTGGAATTAGATGATCTAACAGCTTCAACAAATAGTATTATGACTCTCCTTTCATCCATGTTAACTTACCCTCAGGTCCAGCAATGCAGAACCTACAGAGAGTTCTATGGAAGATTCATCTACTACAGATCGATTAAATTTTGA
Peptide sequence:
MEDSSTTDRLNFDRFDICRLLQHGASLLGSAGAEFEVQDEKSGEVDPKERIARQRKLLQKKLGLNMGEAIGMSTEELFNDEDLDYTPTSAALVNKQSTLQAAELIDSEFRAGMSNRQKNKAKRMAKLFAKQRSRDAVETNEKSNDSTDGEPEEKRRKVANVVINQTASDSKVLIDNVPESSSLIEETNEWPLESFCEELCNDLFNPSWEVRHGAGTGLREILKAHGKSGGKMGDSTLEEMIQQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVVLKHMNETGVHKTVDVLLKLLTQEQWEVRHGGLLGIKYALAVRQDVINTLLPKVLTRIIEGLQDLDDDVRAVAAASLVPVVESLVYLQTQKVPSIINTLWDSLLELDDLTASTNSIMTLLSSMLTYPQVQQCRTYREFYGRFIYYRSIKF
IRESfinder result
IRESfinder score
0.630668094
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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