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mmu_circBrwd1_1380
Basic information of circular RNA
PanCircBase ID:
mmu_circBrwd1_1380
mm39_Chr. Position ID:
chr16|95842451|95858703|-
Gene name:
Brwd1
Transcript:
ENSMUST00000099502.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
21,20,19,18,17,16,15,14,13,12
Exon/intron offsets:
0,1749,3212,5093,5683,6067,11273,12580,14863,16211
Genomic length:
16252
Exon/intron sizes in nt:
132,94,184,189,226,129,135,151,99,41
Flanking exon/introns:
chr16:95839185-95842451|chr16:95858703-95859882
Splice length:
1380
Other database IDs:
CIRCpedia: HSA_CIRCpedia_153035
Human Ortholog IDs:
Mature spliced Sequence:
GACAAAGTTGATAGTATCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTTTGAGCAGTTGGAATGGAGAAGCATTTTACTGGACATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAAGAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAGCCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACATGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGACAAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCACGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTTCATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCACCTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATTAGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGATGGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCGTCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGACTGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATTCAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACGCTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGCACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTATGTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGTCTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACGGACGTGTAGGCGTAAA
59.69
CCAGATCCGTGCTGTTCCAT
60.11
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACGGACGTGTAGGCGTAAA
59.69
TGCTGTTCCATCTCGGCTAC
59.82
170
PCR template sequence:
ACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGACAAAGTTGATAGTATCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTTTGAGCAGTTGGAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-155-5p(1), mmu-miR-221-3p(1), mmu-miR-135a-5p(1), mmu-miR-326-3p(1), mmu-miR-382-3p(1), mmu-miR-383-5p(1), mmu-miR-342-5p(1), mmu-miR-207(1), mmu-miR-673-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCTGTTAGACATGACAAAGTTGATA
siRNA sets
TATCAACTTTGTCATGTCT, ATCAACTTTGTCATGTCTA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBRWD1_1380_ORF_1
1.0194
0.157411271
0.999999915
2640
ORF sequence:
ATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAAGAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAGCCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACATGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGACAAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCACGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTTCATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCACCTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATTAGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGATGGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCGTCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGACTGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATTCAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACGCTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGCACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTATGTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGTCTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGACAAAGTTGATAGTATCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTTTGAGCAGTTGGAATGGAGAAGCATTTTACTGGACATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAAGAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAGCCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACATGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGATGGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGACAAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCACGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTTCATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCACCTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATTAGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGATGGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCGTCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGACTGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATTCAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACGCTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGCACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTATGTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGTCTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATCTTCTGGAAATGAATCTTCAGGCTCTGTTAGACAT
Peptide sequence:
MSARISGDTSSEEERFMKPKVTMIAWNQDDSTVVTAVNDHVLKVWSSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGIKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLHTNDSGDASPESSVLDGMIRQLQQQQDQRLGVDQDGTANGLPSGEGTPRRGSFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEAPPGMFRRLEDIRIERGEEERNLYVIGRKKKTFQVPQKSNSMVLVSQSRQRTCRRKYANYSRRNADQCELSSGNESSGSVRHDKVDSIQFCNNGDRFLSGSRDGTARIWRFEQLEWRSILLDMSARISGDTSSEEERFMKPKVTMIAWNQDDSTVVTAVNDHVLKVWSSYTGQLLHNLLGHADEVFVLETHPFDSRIMLSAGHDGSIFIWDITKGIKMKHYFNMIEGQGHGAVFDCKFSQDGQHFACTDSHGHLLIFGFGCSKPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPTKFQRLVPGRENSADEHLVPQLGYVATSDGEVIEQIISLHTNDSGDASPESSVLDGMIRQLQQQQDQRLGVDQDGTANGLPSGEGTPRRGSFRRLSLDIQSPPNIGLRRSGQVEGVRQMHQNAPRSQIATERDLQAWKRRVVVPEAPPGMFRRLEDIRIERGEEERNLYVIGRKKKTFQVPQKSNSMVLVSQSRQRTCRRKYANYSRRNADQCELSSGNESSGSVRH
IRESfinder result
IRESfinder score
0.467095832
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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