top of page

mmu_circBirc6_564

Basic information of circular RNA
PanCircBase ID:

mmu_circBirc6_564

mm39_Chr. Position ID:

chr17|74999178|75004514|+

Gene name:

Birc6

Transcript:

ENSMUST00000180037.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

4

Exon/intron indexes:

69,70,71,72

Exon/intron offsets:

0,1264,4053,5201

Genomic length:

5336

Exon/intron sizes in nt:

162,78,189,135

Flanking exon/introns:

chr17:74998214-74999178|chr17:75004514-75009198

Splice length:

564

Other database IDs:

​

circAtlas: hsa-BIRC6_0183, CIRCpedia: HSA_CIRCpedia_91719

Human Ortholog IDs:
Mature spliced Sequence:

GTTCTGATAACCGGGCCAGCGGACACCCCCTATGCAAATGGCTGCTTTGAGTTTGATGTATATTTTCCTCAAGATTATCCTAGCTCACCTCCTCTAGTGAACCTAGAAACAACTGGGGGTCATAGTGTGCGCTTCAATCCCAACCTTTACAATGATGGCAAGGTTTGTTTAAGCATCCTGAATACATGGCATGGAAGACCAGAAGAGAAGTGGAACCCTCAGACATCAAGCTTTTTGCAAGTGTTGGTTTCTGTCCAGTCCCTTATATTAGTAGCTGAGCCTTACTTCAATGAACCAGGATATGAACGGTCTAGAGGCACTCCCAGTGGTACACAGAGCTCTCGAGAATACGATGGGAACATCCGACAAGCAACGGTTAAATGGGCTATGCTAGAACAAATCAGAAATCCTTCACCGTGCTTTAAAGAAGTTATACACAAACATTTTTACTTGAAGAGAATTGAGCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CATGGCCCAGTGTGAGGAG

60.08

AGCCATTTGCATAGGGGGTG

60.4

140

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CATGGCCCAGTGTGAGGAG

60.08

CAGCCATTTGCATAGGGGGT

60.4

141

PCR template sequence:

GCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAGGTTCTGATAACCGGGCCAGCGGACACCCCCTATGCAAATGGCTGCTTTGAGTTTGATGTATATTTTCCTCAAGATTATCCTAGCTCACCTCCTCTAGTGA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

2

Interacting miRNAs (#binding sites)

mmu-miR-541-5p(1), mmu-miR-296-3p(1)

siRNAs for circular RNA silencing
Junction sequence

AGCTGCTCTCAAGGTTCTGATAACCG

siRNA sets

TTATCAGAACCTTGAGAGC, ATCAGAACCTTGAGAGCAG, TATCAGAACCTTGAGAGCA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBIRC6_564_ORF_1

0.8267

0.144780053

0.900886639

741

ORF sequence:

ATGCTAGAACAAATCAGAAATCCTTCACCGTGCTTTAAAGAAGTTATACACAAACATTTTTACTTGAAGAGAATTGAGCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAGGTTCTGATAACCGGGCCAGCGGACACCCCCTATGCAAATGGCTGCTTTGAGTTTGATGTATATTTTCCTCAAGATTATCCTAGCTCACCTCCTCTAGTGAACCTAGAAACAACTGGGGGTCATAGTGTGCGCTTCAATCCCAACCTTTACAATGATGGCAAGGTTTGTTTAAGCATCCTGAATACATGGCATGGAAGACCAGAAGAGAAGTGGAACCCTCAGACATCAAGCTTTTTGCAAGTGTTGGTTTCTGTCCAGTCCCTTATATTAGTAGCTGAGCCTTACTTCAATGAACCAGGATATGAACGGTCTAGAGGCACTCCCAGTGGTACACAGAGCTCTCGAGAATACGATGGGAACATCCGACAAGCAACGGTTAAATGGGCTATGCTAGAACAAATCAGAAATCCTTCACCGTGCTTTAAAGAAGTTATACACAAACATTTTTACTTGAAGAGAATTGAGCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAG

Peptide sequence:

MLEQIRNPSPCFKEVIHKHFYLKRIELMAQCEEWIADIQQYSSDKRVGRTMSHHAAALKVLITGPADTPYANGCFEFDVYFPQDYPSSPPLVNLETTGGHSVRFNPNLYNDGKVCLSILNTWHGRPEEKWNPQTSSFLQVLVSVQSLILVAEPYFNEPGYERSRGTPSGTQSSREYDGNIRQATVKWAMLEQIRNPSPCFKEVIHKHFYLKRIELMAQCEEWIADIQQYSSDKRVGRTMSHHAAALK

IRESfinder result

IRESfinder score

0.612323577

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

bottom of page