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mmu_circBirc6_306
Basic information of circular RNA
PanCircBase ID:
mmu_circBirc6_306
mm39_Chr. Position ID:
chr17|74864895|74868257|+
Gene name:
Birc6
Transcript:
ENSMUST00000180037.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
4,5
Exon/intron offsets:
0,3250
Genomic length:
3362
Exon/intron sizes in nt:
194,112
Flanking exon/introns:
chr17:74855845-74864895|chr17:74868257-74869013
Splice length:
306
Other database IDs:
CIRCpedia: MMU_CIRCpedia_28773, circAtlas: mmu-Birc6_0012
circBase: hsa_circ_0119799, circAtlas: hsa-BIRC6_0018, CIRCpedia: HSA_CIRCpedia_40794
Human Ortholog IDs:
Mature spliced Sequence:
TGGGCCACAGTTACATTTCATCTTCCTCATCACGTATTGAAGTCTATTGCCAGTGCCATTGTAAATGAACTTAAGAAAATAAATCAAAATGTTGCTGCCTTACCTGTGGCGTCCTCAGTGATGGACAGATTGTCTTACCTCTTGCCCAGTGCACGTCCAGAACTTGGAGTTGGGCCAGGCCGTTCTGTAGACAGGGCATTGATGTATAGTGAAGCTAACAGAAGGGAGACCTTCACTTCGTGGCCTCATGTTGGCTATAGGTGGGCACAGCCAGATCCCATGGCTCAAGCTGGATTTTATCACCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGAGACCTTCACTTCGTGGC
60.67
TGGCACTGGCAATAGACTTCA
59.65
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCTTCACTTCGTGGCCTCAT
60.04
TCATTTACAATGGCACTGGCA
58.48
145
PCR template sequence:
TAGTGAAGCTAACAGAAGGGAGACCTTCACTTCGTGGCCTCATGTTGGCTATAGGTGGGCACAGCCAGATCCCATGGCTCAAGCTGGATTTTATCACCAGTGGGCCACAGTTACATTTCATCTTCCTCATCACGTATTGAAGTCTATTGCCAGTGCCATTGTAAATGAACTTAAGAAAATAAATCAAAATGTTGCTGCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-15b-5p(1), mmu-miR-339-5p(1), mmu-miR-411-3p(1), mmu-miR-183-5p(1), mmu-miR-532-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ATTTTATCACCAGTGGGCCACAGTTA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBIRC6_306_ORF_1
0.8605
-0.045397309
0.427421795
492
ORF sequence:
ATGGACAGATTGTCTTACCTCTTGCCCAGTGCACGTCCAGAACTTGGAGTTGGGCCAGGCCGTTCTGTAGACAGGGCATTGATGTATAGTGAAGCTAACAGAAGGGAGACCTTCACTTCGTGGCCTCATGTTGGCTATAGGTGGGCACAGCCAGATCCCATGGCTCAAGCTGGATTTTATCACCAGTGGGCCACAGTTACATTTCATCTTCCTCATCACGTATTGAAGTCTATTGCCAGTGCCATTGTAAATGAACTTAAGAAAATAAATCAAAATGTTGCTGCCTTACCTGTGGCGTCCTCAGTGATGGACAGATTGTCTTACCTCTTGCCCAGTGCACGTCCAGAACTTGGAGTTGGGCCAGGCCGTTCTGTAGACAGGGCATTGATGTATAGTGAAGCTAACAGAAGGGAGACCTTCACTTCGTGGCCTCATGTTGGCTATAGGTGGGCACAGCCAGATCCCATGGCTCAAGCTGGATTTTATCACCAG
Peptide sequence:
MDRLSYLLPSARPELGVGPGRSVDRALMYSEANRRETFTSWPHVGYRWAQPDPMAQAGFYHQWATVTFHLPHHVLKSIASAIVNELKKINQNVAALPVASSVMDRLSYLLPSARPELGVGPGRSVDRALMYSEANRRETFTSWPHVGYRWAQPDPMAQAGFYHQ
IRESfinder result
IRESfinder score
0.653940588
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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