top of page

mmu_circBirc6_1587

Basic information of circular RNA
PanCircBase ID:

mmu_circBirc6_1587

mm39_Chr. Position ID:

chr17|74975753|75004514|+

Gene name:

Birc6

Transcript:

ENSMUST00000180037.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

10

Exon/intron indexes:

63,64,65,66,67,68,69,70,71,72

Exon/intron offsets:

0,1455,8407,20459,21599,22243,23425,24689,27478,28626

Genomic length:

28761

Exon/intron sizes in nt:

16,51,69,21,41,46,11,10,00,00,00,00,00,000

Flanking exon/introns:

chr17:74969813-74975753|chr17:75004514-75009198

Splice length:

1587

Other database IDs:

circBase: mmu_circ_0007008, CIRCpedia: MMU_CIRCpedia_28835, circAtlas: mmu-Birc6_0088

Human Ortholog IDs:
Mature spliced Sequence:

CCGCAGGTGTCAAATTCCCATAACCCCACATCAGCAGAAGAACAGCAGCTGTACTGGGCCAAAGGGACTGGCTTTGGCACAGGTTCCACGGCATCAGGATGGGACGTGGAACAAGCCTTAACTAAACAGAGGCTAGAAGAGGAGCATGTGACCTGCCTTTTGCAGGTCCTTGCGAGTTACATAAACCCCATGAGTGGTGCTGTCAATGGGGAAGCCCAGGCGTCTCCCGAGAGCCGCGCCCAGAACAGCAGTGCTCTGCCCTCGGTGCTTCTGGAGCTCCTCAGTCAGTCCTGCCTCATCCCAGCCATGTCCTCCTACCTGCGCAACGACTCCGTTCTGGACATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCTTTCTACAGAGAATGGTGAAGAAGAGGAAGATGAACAGTCAGAATGTCAAACTTCTGTTGGTACATTATTAGCTAAAATGAAGACCTGTGTCGATACCTACACCAACCGCCTAAGATCTAAAAGGGAAAATGTGAAAGCCGGAGTCAAGCCGGACGCGCCTGATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAAGAAAAAAAACTAGGTGAATACTCAAAGAAGGTGGTTATGAAACCCAAACCTCTGTCAGTATTAAAATCACTTGAAGAAAAATATGTGGCTGTCATGAAAAAACTGCAGTTTGATACATTTGAAATGGTTTCTGAAGATGACGATGGGAAATTGGGTTTTAAAGTAAATTACCACTACATGTCTCAGGTGAAAAATGCCAACGATGCCAACAGTGCTGCCAGAGCCCGCCGCCTGGCTCAAGAAGCGGTGACACTGTCAACCTCACTGCCTCTGTCATCGTCTTCCAGTGTCTTTGTACGCTGTGATGAGGAGCGACTTGACATCATGAAGGTTCTGATAACCGGGCCAGCGGACACCCCCTATGCAAATGGCTGCTTTGAGTTTGATGTATATTTTCCTCAAGATTATCCTAGCTCACCTCCTCTAGTGAACCTAGAAACAACTGGGGGTCATAGTGTGCGCTTCAATCCCAACCTTTACAATGATGGCAAGGTTTGTTTAAGCATCCTGAATACATGGCATGGAAGACCAGAAGAGAAGTGGAACCCTCAGACATCAAGCTTTTTGCAAGTGTTGGTTTCTGTCCAGTCCCTTATATTAGTAGCTGAGCCTTACTTCAATGAACCAGGATATGAACGGTCTAGAGGCACTCCCAGTGGTACACAGAGCTCTCGAGAATACGATGGGAACATCCGACAAGCAACGGTTAAATGGGCTATGCTAGAACAAATCAGAAATCCTTCACCGTGCTTTAAAGAAGTTATACACAAACATTTTTACTTGAAGAGAATTGAGCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

TGGATTGCAGACATCCAGCA

59.67

GTCCCTTTGGCCCAGTACAG

60.32

146

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGGATTGCAGACATCCAGCA

59.67

CAGTCCCTTTGGCCCAGTAC

60.32

148

PCR template sequence:

GCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAGCCGCAGGTGTCAAATTCCCATAACCCCACATCAGCAGAAGAACAGCAGCTGTACTGGGCCAAAGGGACTGGCTTTGGCACAGGTTCCACGGCATCAGGAT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

17

Interacting miRNAs (#binding sites)

mmu-miR-204-5p(2), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-34c-5p(1), mmu-let-7c-5p(1), mmu-miR-211-5p(1), mmu-let-7d-5p(1), mmu-miR-433-3p(1), mmu-miR-676-3p(1), mmu-miR-370-3p(1), mmu-miR-541-5p(1), mmu-miR-335-3p(1), mmu-miR-697(1), mmu-miR-1904(1), mmu-miR-296-3p(1), mmu-miR-542-5p(1)

siRNAs for circular RNA silencing
Junction sequence

AGCTGCTCTCAAGCCGCAGGTGTCAA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBIRC6_1587_ORF_1

0.9284

0.221958173

0.999999994

2985

ORF sequence:

ATGAGTGGTGCTGTCAATGGGGAAGCCCAGGCGTCTCCCGAGAGCCGCGCCCAGAACAGCAGTGCTCTGCCCTCGGTGCTTCTGGAGCTCCTCAGTCAGTCCTGCCTCATCCCAGCCATGTCCTCCTACCTGCGCAACGACTCCGTTCTGGACATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCTTTCTACAGAGAATGGTGAAGAAGAGGAAGATGAACAGTCAGAATGTCAAACTTCTGTTGGTACATTATTAGCTAAAATGAAGACCTGTGTCGATACCTACACCAACCGCCTAAGATCTAAAAGGGAAAATGTGAAAGCCGGAGTCAAGCCGGACGCGCCTGATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAAGAAAAAAAACTAGGTGAATACTCAAAGAAGGTGGTTATGAAACCCAAACCTCTGTCAGTATTAAAATCACTTGAAGAAAAATATGTGGCTGTCATGAAAAAACTGCAGTTTGATACATTTGAAATGGTTTCTGAAGATGACGATGGGAAATTGGGTTTTAAAGTAAATTACCACTACATGTCTCAGGTGAAAAATGCCAACGATGCCAACAGTGCTGCCAGAGCCCGCCGCCTGGCTCAAGAAGCGGTGACACTGTCAACCTCACTGCCTCTGTCATCGTCTTCCAGTGTCTTTGTACGCTGTGATGAGGAGCGACTTGACATCATGAAGGTTCTGATAACCGGGCCAGCGGACACCCCCTATGCAAATGGCTGCTTTGAGTTTGATGTATATTTTCCTCAAGATTATCCTAGCTCACCTCCTCTAGTGAACCTAGAAACAACTGGGGGTCATAGTGTGCGCTTCAATCCCAACCTTTACAATGATGGCAAGGTTTGTTTAAGCATCCTGAATACATGGCATGGAAGACCAGAAGAGAAGTGGAACCCTCAGACATCAAGCTTTTTGCAAGTGTTGGTTTCTGTCCAGTCCCTTATATTAGTAGCTGAGCCTTACTTCAATGAACCAGGATATGAACGGTCTAGAGGCACTCCCAGTGGTACACAGAGCTCTCGAGAATACGATGGGAACATCCGACAAGCAACGGTTAAATGGGCTATGCTAGAACAAATCAGAAATCCTTCACCGTGCTTTAAAGAAGTTATACACAAACATTTTTACTTGAAGAGAATTGAGCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAGCCGCAGGTGTCAAATTCCCATAACCCCACATCAGCAGAAGAACAGCAGCTGTACTGGGCCAAAGGGACTGGCTTTGGCACAGGTTCCACGGCATCAGGATGGGACGTGGAACAAGCCTTAACTAAACAGAGGCTAGAAGAGGAGCATGTGACCTGCCTTTTGCAGGTCCTTGCGAGTTACATAAACCCCATGAGTGGTGCTGTCAATGGGGAAGCCCAGGCGTCTCCCGAGAGCCGCGCCCAGAACAGCAGTGCTCTGCCCTCGGTGCTTCTGGAGCTCCTCAGTCAGTCCTGCCTCATCCCAGCCATGTCCTCCTACCTGCGCAACGACTCCGTTCTGGACATGGCAAGACATGTGCCACTCTATCGTGCTCTGCTAGAGTTGCTCCGCGCCATTGCTTCTTGCACATCTATGGTGCCGCTGTTGTTGCCTCTTTCTACAGAGAATGGTGAAGAAGAGGAAGATGAACAGTCAGAATGTCAAACTTCTGTTGGTACATTATTAGCTAAAATGAAGACCTGTGTCGATACCTACACCAACCGCCTAAGATCTAAAAGGGAAAATGTGAAAGCCGGAGTCAAGCCGGACGCGCCTGATCAAGAGCCCGAAGGACTTGCGCTTCTGGTCCCCGACATCCAGAGGACTGCTGAGATCGTCCACGCCGCCACCGCCAATCTGCGGCAAGCCAATCAAGAAAAAAAACTAGGTGAATACTCAAAGAAGGTGGTTATGAAACCCAAACCTCTGTCAGTATTAAAATCACTTGAAGAAAAATATGTGGCTGTCATGAAAAAACTGCAGTTTGATACATTTGAAATGGTTTCTGAAGATGACGATGGGAAATTGGGTTTTAAAGTAAATTACCACTACATGTCTCAGGTGAAAAATGCCAACGATGCCAACAGTGCTGCCAGAGCCCGCCGCCTGGCTCAAGAAGCGGTGACACTGTCAACCTCACTGCCTCTGTCATCGTCTTCCAGTGTCTTTGTACGCTGTGATGAGGAGCGACTTGACATCATGAAGGTTCTGATAACCGGGCCAGCGGACACCCCCTATGCAAATGGCTGCTTTGAGTTTGATGTATATTTTCCTCAAGATTATCCTAGCTCACCTCCTCTAGTGAACCTAGAAACAACTGGGGGTCATAGTGTGCGCTTCAATCCCAACCTTTACAATGATGGCAAGGTTTGTTTAAGCATCCTGAATACATGGCATGGAAGACCAGAAGAGAAGTGGAACCCTCAGACATCAAGCTTTTTGCAAGTGTTGGTTTCTGTCCAGTCCCTTATATTAGTAGCTGAGCCTTACTTCAATGAACCAGGATATGAACGGTCTAGAGGCACTCCCAGTGGTACACAGAGCTCTCGAGAATACGATGGGAACATCCGACAAGCAACGGTTAAATGGGCTATGCTAGAACAAATCAGAAATCCTTCACCGTGCTTTAAAGAAGTTATACACAAACATTTTTACTTGAAGAGAATTGAGCTCATGGCCCAGTGTGAGGAGTGGATTGCAGACATCCAGCAGTACAGCAGTGACAAGCGGGTGGGCAGGACCATGTCTCACCATGCAGCTGCTCTCAAG

Peptide sequence:

MSGAVNGEAQASPESRAQNSSALPSVLLELLSQSCLIPAMSSYLRNDSVLDMARHVPLYRALLELLRAIASCTSMVPLLLPLSTENGEEEEDEQSECQTSVGTLLAKMKTCVDTYTNRLRSKRENVKAGVKPDAPDQEPEGLALLVPDIQRTAEIVHAATANLRQANQEKKLGEYSKKVVMKPKPLSVLKSLEEKYVAVMKKLQFDTFEMVSEDDDGKLGFKVNYHYMSQVKNANDANSAARARRLAQEAVTLSTSLPLSSSSSVFVRCDEERLDIMKVLITGPADTPYANGCFEFDVYFPQDYPSSPPLVNLETTGGHSVRFNPNLYNDGKVCLSILNTWHGRPEEKWNPQTSSFLQVLVSVQSLILVAEPYFNEPGYERSRGTPSGTQSSREYDGNIRQATVKWAMLEQIRNPSPCFKEVIHKHFYLKRIELMAQCEEWIADIQQYSSDKRVGRTMSHHAAALKPQVSNSHNPTSAEEQQLYWAKGTGFGTGSTASGWDVEQALTKQRLEEEHVTCLLQVLASYINPMSGAVNGEAQASPESRAQNSSALPSVLLELLSQSCLIPAMSSYLRNDSVLDMARHVPLYRALLELLRAIASCTSMVPLLLPLSTENGEEEEDEQSECQTSVGTLLAKMKTCVDTYTNRLRSKRENVKAGVKPDAPDQEPEGLALLVPDIQRTAEIVHAATANLRQANQEKKLGEYSKKVVMKPKPLSVLKSLEEKYVAVMKKLQFDTFEMVSEDDDGKLGFKVNYHYMSQVKNANDANSAARARRLAQEAVTLSTSLPLSSSSSVFVRCDEERLDIMKVLITGPADTPYANGCFEFDVYFPQDYPSSPPLVNLETTGGHSVRFNPNLYNDGKVCLSILNTWHGRPEEKWNPQTSSFLQVLVSVQSLILVAEPYFNEPGYERSRGTPSGTQSSREYDGNIRQATVKWAMLEQIRNPSPCFKEVIHKHFYLKRIELMAQCEEWIADIQQYSSDKRVGRTMSHHAAALK

IRESfinder result

IRESfinder score

0.453491005

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

bottom of page