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mmu_circBicral_2448

Basic information of circular RNA
PanCircBase ID:

mmu_circBicral_2448

mm39_Chr. Position ID:

chr17|47117477|47141922|-

Gene name:

Bicral

Transcript:

ENSMUST00000233537.2

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

10

Exon/intron indexes:

11,10,9,8,7,6,5,4,3,2

Exon/intron offsets:

0,844,1797,3016,5372,7381,17898,23360,23513,24399

Genomic length:

24445

Exon/intron sizes in nt:

103,95,146,59,101,106,1674,69,49,46

Flanking exon/introns:

chr17:47112755-47117477|chr17:47141922-47143181

Splice length:

2448

Other database IDs:

circAtlas: mmu-Gltscr1l_0015

​

Human Ortholog IDs:
Mature spliced Sequence:

TTGTCATGGATGATGACGATGACTCCTGTCTCCTCGATCTTATTGGAGACCCACAAGCATTGAACTATTTTCTGCACGGACCTAGCAGTAAATCGGGCAGCGATGATGTGACGAACGCAGGGTATTCTGCAGCCAATTCTAATTCAATTTTCGCCAACTCCACGAACGCTGACCCTAAATCGGCCCTCAAAGGTGTGAGTGACCAGCTTGGGGAGGGGCCCAGTGATGGGCTGCCGCTTGCAAGCAGCCTTCAGTTTCTTGAAGATGAACTTGAGTCTTCACCTCTCCCCGATCTCAGCGAGGACCAACCCTTTGACATTCTTCAGAAATCCTTGCAGGAGGCTAATATCACTGAACAGACATTGGCAGAAGAGGCGTACCTGGATGCCAGTATAGGCTCAAGCCAACAGTTTGCACAAGCCCAGCTTCATCCTTCTTCATCAGCATCCTTTACTCAGGCTTCTAATGTTTCTAATTACTCAGGTCAGACACTGCAGCCTATCGGGGTGACTCACGTGCCTGTTGGAGCATCGTTTGCAAGCAATACAGTGGGTGTGCAGCATGGCTTTATGCAACACGTGGGGATCAGTGTTCCCAGCCAGCATTTGCCTAACAGCAGCCAGATTAGTGGCTCCGGTCAGATACAGTTAATCGGGTCCTTCGGTAATCAGCCTTCCATGATGACTATAAATAACCTCGATGGCTCTCAAATCATACTGAAAGGCAGTGGGCAGCAAGCCCCATCTAATGTGAGTGGGGGGCTTCTGGTTCACAGACAGACTCCTAACGGCAACTCTCTGTTTGGGAACTCCACTTCCAGTCCTGTAGCACAGCCTGTCACCGTTCCATTTAACAGCACAAATTTCCAGGCATCTTTACCCGTGCATAACATCATTATTCAAAGGGGTCTTGCACCAAATTCAAATAAAGTCCCAATTAATATCCAGCCAAAGCCGGTCCAGATGGGTCAGCAGAGCGCGTACAATGTGAACAACCTTGGGATCCAGCAGCACCATGCCCAGCAGGGGATCTCCTTCGCCCCCACAAGCTCGCCCCAGGGCTCCGTGGTTGGGCCGCACATGTCTGTGAACATTGTCAACCAACAGAACACGAGAAAGCCTGTCACCTCGCAGGCAGTGAGCGGCACAGGGGGCAGCATCGTCATCCATTCCCCCATGGGCCAGCCTCACACTCCCCAAAGTCAGTTCCTTATACCCACAAGCCTTTCTGTCAGCTCCAACTCGGTGCACCATGTCCAGGCTATAAACGGGCAGCTGCTTCAGACTCAGCCCTCCCAGCTCATCTCTGGCCAAGTGGCCTCTGAGCATGTCATGCTGAACAGGAATTCCTCTAACATGCTCAGGACCAACCAACCATATTCCGGACAGATGCTTAATAACCAGAATACCGCCGTCCAGCTGGTGTCTGGGCAGACTTTTGCCACCTCTGGAAGTCCAGTGATAGTCAACCACGCCTCTCCTCAGATCGTCGGGGGACAGATGCCCTTGCAGCAGGCCTCACCCACCGTGTTACACCTGTCACCTGGGCAGAGCAGTGTTTCCCAGGGAAGGCCAGGCTTCGCCACCATGCCCGCGGTGAGCGGCATGGCAGGACCCGCTCGGTTCCCCGCCGTCAGCTCAGCTAGCACTGCTCATCCTACTCTTGGGCCTACGGTGCAGTCGGGGGCACCGGGATCAAACTTTACGGGAGACCAGCTGACACAAGCCAACAGAACGCCAGCGCCCGTCAGTGTGTCCCACCGTCTTCCAGTCTCTGCTTCCAAATCCCCCAGCACCTTGAGCAACACCCCGGGGACACAGCAGCAGTTCTTCTGTCAGGCTCAGAAGAAGTGTTTGAACCAGACCTCCCCCATTCCCACATCCAAGACCACAGACGGCTTGAGGCCATCACAGATCCCTGGGCTCTTGAGCACCGCACTGCCAGGACAGGATTCTGGAAGCAAAATTATGCCAGCGACCTTGGGGGCCACACAGGCACAACCAGAAAGCTCAGTTGGATCATCCCCGAGCCAGACAGCTGTGCAGGTGGATAGTCATCCAGGACAGAAAAGGCCTGCTGCCAAACAGCTGACTAAAGGAGCTTTCATCCTCCAGCAGTTACAGAGGGACCAAGCCCATGCTGTGACACCCGACAAAAGCCAGTTCCGGTCACTAAATGACACGGTGCAGAGACTGCTCTCCTACCACGTGTGCCAGGGCTCCATGCCCACGGAGGAAGACCTGAGGCAAGTGGACAATGAATTTGAAGAGGTCGCCACTCAGCTCCTCAAAAGGACCCAAGCTATGCTGAACAAATACAGATTCCTGCTCCTAGAAGACGCCATGAGGATCAACCCCTCTGCAGAGATGGTGATGATTGACAGGATGTTCAACCAGGAGGAAAGAGCTTCCCTGTCGAGGGACAAGCGTCTGGCGCTCGTAGATCCTG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

CAACCCCTCTGCAGAGATGG

60.11

TCAATGCTTGTGGGTCTCCA

59.23

161

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CAACCCCTCTGCAGAGATGG

60.11

TCAATGCTTGTGGGTCTCCAA

59.86

161

PCR template sequence:

ATCAACCCCTCTGCAGAGATGGTGATGATTGACAGGATGTTCAACCAGGAGGAAAGAGCTTCCCTGTCGAGGGACAAGCGTCTGGCGCTCGTAGATCCTGTTGTCATGGATGATGACGATGACTCCTGTCTCCTCGATCTTATTGGAGACCCACAAGCATTGAACTATTTTCTGCACGGACCTAGCAGTAAATCGGGCAG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

21

Interacting miRNAs (#binding sites)

mmu-miR-34c-5p(2), mmu-miR-23a-3p(2), mmu-miR-370-3p(2), mmu-miR-541-5p(2), mmu-miR-137-3p(1), mmu-miR-23b-3p(1), mmu-miR-296-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-34a-5p(1), mmu-miR-122-5p(1), mmu-miR-298-5p(1), mmu-miR-709(1), mmu-miR-676-3p(1), mmu-miR-22-3p(1), mmu-miR-324-3p(1), mmu-miR-34b-5p(1), mmu-miR-666-5p(1), mmu-miR-673-5p(1), mmu-miR-669d-5p(1), mmu-miR-542-5p(1)

siRNAs for circular RNA silencing
Junction sequence

CTCGTAGATCCTGTTGTCATGGATGA

siRNA sets

ATGACAACAGGATCTACGA, TGACAACAGGATCTACGAG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBICRAL_2448_ORF_1

1.0166

0.221383242

1

4890

ORF sequence:

ATGGATGATGACGATGACTCCTGTCTCCTCGATCTTATTGGAGACCCACAAGCATTGAACTATTTTCTGCACGGACCTAGCAGTAAATCGGGCAGCGATGATGTGACGAACGCAGGGTATTCTGCAGCCAATTCTAATTCAATTTTCGCCAACTCCACGAACGCTGACCCTAAATCGGCCCTCAAAGGTGTGAGTGACCAGCTTGGGGAGGGGCCCAGTGATGGGCTGCCGCTTGCAAGCAGCCTTCAGTTTCTTGAAGATGAACTTGAGTCTTCACCTCTCCCCGATCTCAGCGAGGACCAACCCTTTGACATTCTTCAGAAATCCTTGCAGGAGGCTAATATCACTGAACAGACATTGGCAGAAGAGGCGTACCTGGATGCCAGTATAGGCTCAAGCCAACAGTTTGCACAAGCCCAGCTTCATCCTTCTTCATCAGCATCCTTTACTCAGGCTTCTAATGTTTCTAATTACTCAGGTCAGACACTGCAGCCTATCGGGGTGACTCACGTGCCTGTTGGAGCATCGTTTGCAAGCAATACAGTGGGTGTGCAGCATGGCTTTATGCAACACGTGGGGATCAGTGTTCCCAGCCAGCATTTGCCTAACAGCAGCCAGATTAGTGGCTCCGGTCAGATACAGTTAATCGGGTCCTTCGGTAATCAGCCTTCCATGATGACTATAAATAACCTCGATGGCTCTCAAATCATACTGAAAGGCAGTGGGCAGCAAGCCCCATCTAATGTGAGTGGGGGGCTTCTGGTTCACAGACAGACTCCTAACGGCAACTCTCTGTTTGGGAACTCCACTTCCAGTCCTGTAGCACAGCCTGTCACCGTTCCATTTAACAGCACAAATTTCCAGGCATCTTTACCCGTGCATAACATCATTATTCAAAGGGGTCTTGCACCAAATTCAAATAAAGTCCCAATTAATATCCAGCCAAAGCCGGTCCAGATGGGTCAGCAGAGCGCGTACAATGTGAACAACCTTGGGATCCAGCAGCACCATGCCCAGCAGGGGATCTCCTTCGCCCCCACAAGCTCGCCCCAGGGCTCCGTGGTTGGGCCGCACATGTCTGTGAACATTGTCAACCAACAGAACACGAGAAAGCCTGTCACCTCGCAGGCAGTGAGCGGCACAGGGGGCAGCATCGTCATCCATTCCCCCATGGGCCAGCCTCACACTCCCCAAAGTCAGTTCCTTATACCCACAAGCCTTTCTGTCAGCTCCAACTCGGTGCACCATGTCCAGGCTATAAACGGGCAGCTGCTTCAGACTCAGCCCTCCCAGCTCATCTCTGGCCAAGTGGCCTCTGAGCATGTCATGCTGAACAGGAATTCCTCTAACATGCTCAGGACCAACCAACCATATTCCGGACAGATGCTTAATAACCAGAATACCGCCGTCCAGCTGGTGTCTGGGCAGACTTTTGCCACCTCTGGAAGTCCAGTGATAGTCAACCACGCCTCTCCTCAGATCGTCGGGGGACAGATGCCCTTGCAGCAGGCCTCACCCACCGTGTTACACCTGTCACCTGGGCAGAGCAGTGTTTCCCAGGGAAGGCCAGGCTTCGCCACCATGCCCGCGGTGAGCGGCATGGCAGGACCCGCTCGGTTCCCCGCCGTCAGCTCAGCTAGCACTGCTCATCCTACTCTTGGGCCTACGGTGCAGTCGGGGGCACCGGGATCAAACTTTACGGGAGACCAGCTGACACAAGCCAACAGAACGCCAGCGCCCGTCAGTGTGTCCCACCGTCTTCCAGTCTCTGCTTCCAAATCCCCCAGCACCTTGAGCAACACCCCGGGGACACAGCAGCAGTTCTTCTGTCAGGCTCAGAAGAAGTGTTTGAACCAGACCTCCCCCATTCCCACATCCAAGACCACAGACGGCTTGAGGCCATCACAGATCCCTGGGCTCTTGAGCACCGCACTGCCAGGACAGGATTCTGGAAGCAAAATTATGCCAGCGACCTTGGGGGCCACACAGGCACAACCAGAAAGCTCAGTTGGATCATCCCCGAGCCAGACAGCTGTGCAGGTGGATAGTCATCCAGGACAGAAAAGGCCTGCTGCCAAACAGCTGACTAAAGGAGCTTTCATCCTCCAGCAGTTACAGAGGGACCAAGCCCATGCTGTGACACCCGACAAAAGCCAGTTCCGGTCACTAAATGACACGGTGCAGAGACTGCTCTCCTACCACGTGTGCCAGGGCTCCATGCCCACGGAGGAAGACCTGAGGCAAGTGGACAATGAATTTGAAGAGGTCGCCACTCAGCTCCTCAAAAGGACCCAAGCTATGCTGAACAAATACAGATTCCTGCTCCTAGAAGACGCCATGAGGATCAACCCCTCTGCAGAGATGGTGATGATTGACAGGATGTTCAACCAGGAGGAAAGAGCTTCCCTGTCGAGGGACAAGCGTCTGGCGCTCGTAGATCCTGTTGTCATGGATGATGACGATGACTCCTGTCTCCTCGATCTTATTGGAGACCCACAAGCATTGAACTATTTTCTGCACGGACCTAGCAGTAAATCGGGCAGCGATGATGTGACGAACGCAGGGTATTCTGCAGCCAATTCTAATTCAATTTTCGCCAACTCCACGAACGCTGACCCTAAATCGGCCCTCAAAGGTGTGAGTGACCAGCTTGGGGAGGGGCCCAGTGATGGGCTGCCGCTTGCAAGCAGCCTTCAGTTTCTTGAAGATGAACTTGAGTCTTCACCTCTCCCCGATCTCAGCGAGGACCAACCCTTTGACATTCTTCAGAAATCCTTGCAGGAGGCTAATATCACTGAACAGACATTGGCAGAAGAGGCGTACCTGGATGCCAGTATAGGCTCAAGCCAACAGTTTGCACAAGCCCAGCTTCATCCTTCTTCATCAGCATCCTTTACTCAGGCTTCTAATGTTTCTAATTACTCAGGTCAGACACTGCAGCCTATCGGGGTGACTCACGTGCCTGTTGGAGCATCGTTTGCAAGCAATACAGTGGGTGTGCAGCATGGCTTTATGCAACACGTGGGGATCAGTGTTCCCAGCCAGCATTTGCCTAACAGCAGCCAGATTAGTGGCTCCGGTCAGATACAGTTAATCGGGTCCTTCGGTAATCAGCCTTCCATGATGACTATAAATAACCTCGATGGCTCTCAAATCATACTGAAAGGCAGTGGGCAGCAAGCCCCATCTAATGTGAGTGGGGGGCTTCTGGTTCACAGACAGACTCCTAACGGCAACTCTCTGTTTGGGAACTCCACTTCCAGTCCTGTAGCACAGCCTGTCACCGTTCCATTTAACAGCACAAATTTCCAGGCATCTTTACCCGTGCATAACATCATTATTCAAAGGGGTCTTGCACCAAATTCAAATAAAGTCCCAATTAATATCCAGCCAAAGCCGGTCCAGATGGGTCAGCAGAGCGCGTACAATGTGAACAACCTTGGGATCCAGCAGCACCATGCCCAGCAGGGGATCTCCTTCGCCCCCACAAGCTCGCCCCAGGGCTCCGTGGTTGGGCCGCACATGTCTGTGAACATTGTCAACCAACAGAACACGAGAAAGCCTGTCACCTCGCAGGCAGTGAGCGGCACAGGGGGCAGCATCGTCATCCATTCCCCCATGGGCCAGCCTCACACTCCCCAAAGTCAGTTCCTTATACCCACAAGCCTTTCTGTCAGCTCCAACTCGGTGCACCATGTCCAGGCTATAAACGGGCAGCTGCTTCAGACTCAGCCCTCCCAGCTCATCTCTGGCCAAGTGGCCTCTGAGCATGTCATGCTGAACAGGAATTCCTCTAACATGCTCAGGACCAACCAACCATATTCCGGACAGATGCTTAATAACCAGAATACCGCCGTCCAGCTGGTGTCTGGGCAGACTTTTGCCACCTCTGGAAGTCCAGTGATAGTCAACCACGCCTCTCCTCAGATCGTCGGGGGACAGATGCCCTTGCAGCAGGCCTCACCCACCGTGTTACACCTGTCACCTGGGCAGAGCAGTGTTTCCCAGGGAAGGCCAGGCTTCGCCACCATGCCCGCGGTGAGCGGCATGGCAGGACCCGCTCGGTTCCCCGCCGTCAGCTCAGCTAGCACTGCTCATCCTACTCTTGGGCCTACGGTGCAGTCGGGGGCACCGGGATCAAACTTTACGGGAGACCAGCTGACACAAGCCAACAGAACGCCAGCGCCCGTCAGTGTGTCCCACCGTCTTCCAGTCTCTGCTTCCAAATCCCCCAGCACCTTGAGCAACACCCCGGGGACACAGCAGCAGTTCTTCTGTCAGGCTCAGAAGAAGTGTTTGAACCAGACCTCCCCCATTCCCACATCCAAGACCACAGACGGCTTGAGGCCATCACAGATCCCTGGGCTCTTGAGCACCGCACTGCCAGGACAGGATTCTGGAAGCAAAATTATGCCAGCGACCTTGGGGGCCACACAGGCACAACCAGAAAGCTCAGTTGGATCATCCCCGAGCCAGACAGCTGTGCAGGTGGATAGTCATCCAGGACAGAAAAGGCCTGCTGCCAAACAGCTGACTAAAGGAGCTTTCATCCTCCAGCAGTTACAGAGGGACCAAGCCCATGCTGTGACACCCGACAAAAGCCAGTTCCGGTCACTAAATGACACGGTGCAGAGACTGCTCTCCTACCACGTGTGCCAGGGCTCCATGCCCACGGAGGAAGACCTGAGGCAAGTGGACAATGAATTTGAAGAGGTCGCCACTCAGCTCCTCAAAAGGACCCAAGCTATGCTGAACAAATACAGATTCCTGCTCCTAGAAGACGCCATGAGGATCAACCCCTCTGCAGAGATGGTGATGATTGACAGGATGTTCAACCAGGAGGAAAGAGCTTCCCTGTCGAGGGACAAGCGTCTGGCGCTCGTAGATCCT

Peptide sequence:

MDDDDDSCLLDLIGDPQALNYFLHGPSSKSGSDDVTNAGYSAANSNSIFANSTNADPKSALKGVSDQLGEGPSDGLPLASSLQFLEDELESSPLPDLSEDQPFDILQKSLQEANITEQTLAEEAYLDASIGSSQQFAQAQLHPSSSASFTQASNVSNYSGQTLQPIGVTHVPVGASFASNTVGVQHGFMQHVGISVPSQHLPNSSQISGSGQIQLIGSFGNQPSMMTINNLDGSQIILKGSGQQAPSNVSGGLLVHRQTPNGNSLFGNSTSSPVAQPVTVPFNSTNFQASLPVHNIIIQRGLAPNSNKVPINIQPKPVQMGQQSAYNVNNLGIQQHHAQQGISFAPTSSPQGSVVGPHMSVNIVNQQNTRKPVTSQAVSGTGGSIVIHSPMGQPHTPQSQFLIPTSLSVSSNSVHHVQAINGQLLQTQPSQLISGQVASEHVMLNRNSSNMLRTNQPYSGQMLNNQNTAVQLVSGQTFATSGSPVIVNHASPQIVGGQMPLQQASPTVLHLSPGQSSVSQGRPGFATMPAVSGMAGPARFPAVSSASTAHPTLGPTVQSGAPGSNFTGDQLTQANRTPAPVSVSHRLPVSASKSPSTLSNTPGTQQQFFCQAQKKCLNQTSPIPTSKTTDGLRPSQIPGLLSTALPGQDSGSKIMPATLGATQAQPESSVGSSPSQTAVQVDSHPGQKRPAAKQLTKGAFILQQLQRDQAHAVTPDKSQFRSLNDTVQRLLSYHVCQGSMPTEEDLRQVDNEFEEVATQLLKRTQAMLNKYRFLLLEDAMRINPSAEMVMIDRMFNQEERASLSRDKRLALVDPVVMDDDDDSCLLDLIGDPQALNYFLHGPSSKSGSDDVTNAGYSAANSNSIFANSTNADPKSALKGVSDQLGEGPSDGLPLASSLQFLEDELESSPLPDLSEDQPFDILQKSLQEANITEQTLAEEAYLDASIGSSQQFAQAQLHPSSSASFTQASNVSNYSGQTLQPIGVTHVPVGASFASNTVGVQHGFMQHVGISVPSQHLPNSSQISGSGQIQLIGSFGNQPSMMTINNLDGSQIILKGSGQQAPSNVSGGLLVHRQTPNGNSLFGNSTSSPVAQPVTVPFNSTNFQASLPVHNIIIQRGLAPNSNKVPINIQPKPVQMGQQSAYNVNNLGIQQHHAQQGISFAPTSSPQGSVVGPHMSVNIVNQQNTRKPVTSQAVSGTGGSIVIHSPMGQPHTPQSQFLIPTSLSVSSNSVHHVQAINGQLLQTQPSQLISGQVASEHVMLNRNSSNMLRTNQPYSGQMLNNQNTAVQLVSGQTFATSGSPVIVNHASPQIVGGQMPLQQASPTVLHLSPGQSSVSQGRPGFATMPAVSGMAGPARFPAVSSASTAHPTLGPTVQSGAPGSNFTGDQLTQANRTPAPVSVSHRLPVSASKSPSTLSNTPGTQQQFFCQAQKKCLNQTSPIPTSKTTDGLRPSQIPGLLSTALPGQDSGSKIMPATLGATQAQPESSVGSSPSQTAVQVDSHPGQKRPAAKQLTKGAFILQQLQRDQAHAVTPDKSQFRSLNDTVQRLLSYHVCQGSMPTEEDLRQVDNEFEEVATQLLKRTQAMLNKYRFLLLEDAMRINPSAEMVMIDRMFNQEERASLSRDKRLALVDP

IRESfinder result

IRESfinder score

0.46340092

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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