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mmu_circBfar_522
Basic information of circular RNA
PanCircBase ID:
mmu_circBfar_522
mm39_Chr. Position ID:
chr16|13510296|13516939|+
Gene name:
Bfar
Transcript:
ENSMUST00000023365.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,4900,6440
Genomic length:
6643
Exon/intron sizes in nt:
14,51,74,203
Flanking exon/introns:
chr16:13506789-13510296|chr16:13516939-13519928
Splice length:
522
Other database IDs:
CIRCpedia: MMU_CIRCpedia_8484, circAtlas: mmu-Bfar_0003
circBase: hsa_circ_0037984, circAtlas: hsa-BFAR_0004, CIRCpedia: HSA_CIRCpedia_16450
Human Ortholog IDs:
Mature spliced Sequence:
GTTGCTTTTAACTTTGACAGAAGAAGAGTTTTCCAGGGCACCTTATACCATAGAGAACAGTAGCCACAGAAGGGTCATCCTCACGGAGCTGGAGCGTGTGAGAGCCCTGGGAGTGAAGCCACCGCAGAATCTCTGGGAGTACAAGGCTGTCAACCCGGGTAGATCCCTATTCTTGCTCTACGCTCTCAAGAGCTCACCCAGACTTGGCTTGCTATACCTGTACCTGTTTGACTACACAGACTGCTTCCTACCCTTCATCCACACCATCTGCCCTCTGCAGGAAAACAGCTCTGGCGAGGACATTTTCACCAAGCTTCTGGACCTGAGGGAGCCCACGTGGAAGCAGTGGAGAGAATTCCTCGTCAAGTACTCCTTTCTTCCGTACCAGCTGATCGCGGAGTTTGCCTGGGACTGGCTGGAGGTCCACTACTGGACATCTCGCTTCCTGATCGTCAATGCCGTGCTGCTCTCTGTTCTGGAGTTGTTCTCTTTTTGGAGGATCTGGTCGAGAAGTGAGCTGAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCCTGATCGTCAATGCCGT
59.75
AGGATGACCCTTCTGTGGCT
60.55
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGCTTCCTGATCGTCAATGC
59.7
AGGATGACCCTTCTGTGGCT
60.55
164
PCR template sequence:
TCCACTACTGGACATCTCGCTTCCTGATCGTCAATGCCGTGCTGCTCTCTGTTCTGGAGTTGTTCTCTTTTTGGAGGATCTGGTCGAGAAGTGAGCTGAAGTTGCTTTTAACTTTGACAGAAGAAGAGTTTTCCAGGGCACCTTATACCATAGAGAACAGTAGCCACAGAAGGGTCATCCTCACGGAGCTGGAGCGTGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-15b-5p(1), mmu-miR-298-5p(1), mmu-miR-541-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGTGAGCTGAAGTTGCTTTTAACT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.730085532
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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