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mmu_circBcap31_521
Basic information of circular RNA
PanCircBase ID:
mmu_circBcap31_521
mm39_Chr. Position ID:
chrX|72733152|72758534|-
Gene name:
Bcap31
Transcript:
ENSMUST00000002091.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,4,3,2
Exon/intron offsets:
0,17450,22898,25246
Genomic length:
25382
Exon/intron sizes in nt:
1,36,14,81,01,136
Flanking exon/introns:
chrX:72732386-72733152|chrX:72758534-72759723
Splice length:
521
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GAAACAAGCTCCCATCCCTCTGTTGGAACCCTTTAAGTCACAGGATGAGTTTGCAGTGGACTACAGTTGCCACCTTCCTCTACGCAGAGGTCTTTGCTGTGTTGCTTCTCTGCATTCCCTTCATTTCTCCAAAAAGATGGCAGAAGGTTTTTAAATCCCGGCTGGTGGAGTTGGTAGTGACCTATGGCAACACTTTCTTTGTGGTTCTCATCGTCATCCTTGTACTGTTGGTTATTGATGCTGTACGAGAGATCCTGAAATACGATGATGTGACAGAAAAGGTGAACCTCCAGAACAATCCAGGTGCCATGGAGCACTTCCACATGAAGCTTTTCCGTGCTCAGAGGAATCTCTATATTGCTGGCTTTTCCTTGCTGCTGTCCTTCCTGCTTAGACGCCTGGTGACTCTCATCTCCCAGCAGGCCACACTGCTGGCCTCCAATGAAGCCTTTAAAAAGCAGGCAGAAAGTGCCAGTGAGGCGGCCAAGAAATACATGGAGGAGAATGATCAGCTAAAGAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCCTCCAATGAAGCCTTT
59.89
GGCAACTGTAGTCCACTGCA
60.25
160
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCCTCCAATGAAGCCTTT
59.89
TGGCAACTGTAGTCCACTGC
60.25
161
PCR template sequence:
GGCCACACTGCTGGCCTCCAATGAAGCCTTTAAAAAGCAGGCAGAAAGTGCCAGTGAGGCGGCCAAGAAATACATGGAGGAGAATGATCAGCTAAAGAAGGAAACAAGCTCCCATCCCTCTGTTGGAACCCTTTAAGTCACAGGATGAGTTTGCAGTGGACTACAGTTGCCACCTTCCTCTACGCAGAGGTCTTTGCTGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-16-5p(1), mmu-miR-1a-3p(1), mmu-miR-34c-5p(1), mmu-miR-101a-3p(1), mmu-miR-370-3p(1), mmu-miR-183-5p(1), mmu-miR-1198-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCAGCTAAAGAAGGAAACAAGCTCCC
siRNA sets
TTGTTTCCTTCTTTAGCTG,TGTTTCCTTCTTTAGCTGA,GCTTGTTTCCTTCTTTAGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBCAP31_521_ORF_1
1.1579
0.276353732
0.84783042
513
ORF sequence:
ATGAGTTTGCAGTGGACTACAGTTGCCACCTTCCTCTACGCAGAGGTCTTTGCTGTGTTGCTTCTCTGCATTCCCTTCATTTCTCCAAAAAGATGGCAGAAGGTTTTTAAATCCCGGCTGGTGGAGTTGGTAGTGACCTATGGCAACACTTTCTTTGTGGTTCTCATCGTCATCCTTGTACTGTTGGTTATTGATGCTGTACGAGAGATCCTGAAATACGATGATGTGACAGAAAAGGTGAACCTCCAGAACAATCCAGGTGCCATGGAGCACTTCCACATGAAGCTTTTCCGTGCTCAGAGGAATCTCTATATTGCTGGCTTTTCCTTGCTGCTGTCCTTCCTGCTTAGACGCCTGGTGACTCTCATCTCCCAGCAGGCCACACTGCTGGCCTCCAATGAAGCCTTTAAAAAGCAGGCAGAAAGTGCCAGTGAGGCGGCCAAGAAATACATGGAGGAGAATGATCAGCTAAAGAAGGAAACAAGCTCCCATCCCTCTGTTGGAACCCTTTAA
Peptide sequence:
MSLQWTTVATFLYAEVFAVLLLCIPFISPKRWQKVFKSRLVELVVTYGNTFFVVLIVILVLLVIDAVREILKYDDVTEKVNLQNNPGAMEHFHMKLFRAQRNLYIAGFSLLLSFLLRRLVTLISQQATLLASNEAFKKQAESASEAAKKYMEENDQLKKETSSHPSVGTL
IRESfinder result
IRESfinder score
0.619967599
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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