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mmu_circBbs9_274
Basic information of circular RNA
PanCircBase ID:
mmu_circBbs9_274
mm39_Chr. Position ID:
chr9|22402113|22408155|+
Gene name:
Bbs9
Transcript:
ENSMUST00000039798.16
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
2,3
Exon/intron offsets:
0,5891
Genomic length:
6042
Exon/intron sizes in nt:
1,23,151
Flanking exon/introns:
chr9:22387351-22402113|chr9:22408155-22415340
Splice length:
274
Other database IDs:
circAtlas: mmu-Bbs9_0025
Human Ortholog IDs:
Mature spliced Sequence:
GTTGAAAGGAAATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGAATTTGACCAGGGCTGCTTATGCTTGGCTGATGTGGACAACAGTGGGAACGGCCACGATAAAATCATTGTGGGCAGCTTCATGGGCTACCTGAGAATCTTTAGTCCCCATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTGAAGATTTACTTCTAGAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TAGAAGTGCATCTGCGGGAC
59.82
AAGCATAAGCAGCCCTGGTC
60.39
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTAAAAGCTGGAGGTGGGC
60.25
AGCAGCCCTGGTCAAATTCT
59.59
180
PCR template sequence:
ATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTGAAGATTTACTTCTAGAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTCGTTGAAAGGAAATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGAATTTGACCAGGGCTGCTTATGCTTGGCTGATGT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAAGTTTGTCTCGTTGAAAGGAAAT
siRNA sets
ATTTCCTTTCAACGAGACA,TTTCCTTTCAACGAGACAA,TTCAACGAGACAAACTTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBBS9_274_ORF_1
1.0329
0.14549277
0.340303918
288
ORF sequence:
ATGTCCTTATTTAAAGCCCGTGACTGGTGGTCTACTGTGTTGGGAGAAAAAGAAGAATTTGACCAGGGCTGCTTATGCTTGGCTGATGTGGACAACAGTGGGAACGGCCACGATAAAATCATTGTGGGCAGCTTCATGGGCTACCTGAGAATCTTTAGTCCCCATTCTGTAAAAGCTGGAGGTGGGCCTCAGGCTGAAGATTTACTTCTAGAAGTGCATCTGCGGGACCCAGTGCTGCAAGTGGAAGTCGGGAAGTTTGTCTCGTTGAAAGGAAATGTCCTTATTTAA
Peptide sequence:
MSLFKARDWWSTVLGEKEEFDQGCLCLADVDNSGNGHDKIIVGSFMGYLRIFSPHSVKAGGGPQAEDLLLEVHLRDPVLQVEVGKFVSLKGNVLI
IRESfinder result
IRESfinder score
0.610969647
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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