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mmu_circBbs7_639
Basic information of circular RNA
PanCircBase ID:
mmu_circBbs7_639
mm39_Chr. Position ID:
chr3|36632544|36648709|-
Gene name:
Bbs7
Transcript:
ENSMUST00000040148.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
15,14,13,12,11
Exon/intron offsets:
0,4230,12256,13969,15972
Genomic length:
16165
Exon/intron sizes in nt:
165,140,66,75,193
Flanking exon/introns:
chr3:36630001-36632544|chr3:36648709-36652414
Splice length:
639
Other database IDs:
CIRCpedia: MMU_CIRCpedia_224688, circAtlas: mmu-Bbs7_0009
circAtlas: hsa-BBS7_0024, CIRCpedia: HSA_CIRCpedia_103527
Human Ortholog IDs:
Mature spliced Sequence:
GTCTGAAATAGAACACCTGCAGTTTAAGGTGCTTCAGGAGAGAGAAAATTACCAACAGTCTTCACAGTCAAGCCAAGCAAAGTCAACAGTCCCTTCATTCAGTATAAATGACAAGTTCACATTGAATAAAGAAGATGCTAGCTACAGCCTCGTCTTAGAGGTGCGGACTGCAATAGACAATGTCCTAATACAGAGTGATGTTCCAATAGATCTACTTGATGTGGACAAGAATTCTGCTGTTGTGAGCTTCAGCAGCTGTGACACAGAGTCAAACGACAACTTTCTTCTTGCTACTTATCGGTGCCAGGCAAATACCACCAGGCTGGAGCTCAAGATTCGTTCAATTGAAGGCCAGTATGGCACACTCCAGGCATATGTGACTCCAAGAATCCAACCAAAAACCTGTCAGGTTCGCCAATACCACATCAAACCTCTATCACTCCATCAAAGAACTCACTTTATTGACCATGACAGGCCTATGAACACACTGACTCTAACAGGCCAGTTCAGCTTTGCTGAAGTTCACTCCTGGGTGGTGTTCTGCCTGCCTGAAGTTCCTGAAAAGCCTCCAGCAGGGGAATGTGCAACATTTTACTTTCAGAATACCTTTCTGGATACACAACTTGAGTGTGTATACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTGCCTGAAGTTCCTGAA
60.25
TGCTTGGCTTGACTGTGAAGA
60.13
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCCTGCCTGAAGTTCCTGAA
60.25
GCTTGGCTTGACTGTGAAGAC
59.73
175
PCR template sequence:
TCTGCCTGCCTGAAGTTCCTGAAAAGCCTCCAGCAGGGGAATGTGCAACATTTTACTTTCAGAATACCTTTCTGGATACACAACTTGAGTGTGTATACAGGTCTGAAATAGAACACCTGCAGTTTAAGGTGCTTCAGGAGAGAGAAAATTACCAACAGTCTTCACAGTCAAGCCAAGCAAAGTCAACAGTCCCTTCATTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-26a-5p(1), mmu-miR-122-5p(1), mmu-miR-320-3p(1), mmu-miR-127-5p(1), mmu-miR-382-5p(1), mmu-miR-370-3p(1), mmu-miR-673-5p(1), mmu-miR-3470b(1)
siRNAs for circular RNA silencing
Junction sequence
AGTGTGTATACAGGTCTGAAATAGAA
siRNA sets
TATTTCAGACCTGTATACA, TTCAGACCTGTATACACAC, TTTCAGACCTGTATACACA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBBS7_639_ORF_1
0.8055
0.10370807
0.917404385
798
ORF sequence:
ATGAACACACTGACTCTAACAGGCCAGTTCAGCTTTGCTGAAGTTCACTCCTGGGTGGTGTTCTGCCTGCCTGAAGTTCCTGAAAAGCCTCCAGCAGGGGAATGTGCAACATTTTACTTTCAGAATACCTTTCTGGATACACAACTTGAGTGTGTATACAGGTCTGAAATAGAACACCTGCAGTTTAAGGTGCTTCAGGAGAGAGAAAATTACCAACAGTCTTCACAGTCAAGCCAAGCAAAGTCAACAGTCCCTTCATTCAGTATAAATGACAAGTTCACATTGAATAAAGAAGATGCTAGCTACAGCCTCGTCTTAGAGGTGCGGACTGCAATAGACAATGTCCTAATACAGAGTGATGTTCCAATAGATCTACTTGATGTGGACAAGAATTCTGCTGTTGTGAGCTTCAGCAGCTGTGACACAGAGTCAAACGACAACTTTCTTCTTGCTACTTATCGGTGCCAGGCAAATACCACCAGGCTGGAGCTCAAGATTCGTTCAATTGAAGGCCAGTATGGCACACTCCAGGCATATGTGACTCCAAGAATCCAACCAAAAACCTGTCAGGTTCGCCAATACCACATCAAACCTCTATCACTCCATCAAAGAACTCACTTTATTGACCATGACAGGCCTATGAACACACTGACTCTAACAGGCCAGTTCAGCTTTGCTGAAGTTCACTCCTGGGTGGTGTTCTGCCTGCCTGAAGTTCCTGAAAAGCCTCCAGCAGGGGAATGTGCAACATTTTACTTTCAGAATACCTTTCTGGATACACAACTTGAGTGTGTATAC
Peptide sequence:
MNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVYRSEIEHLQFKVLQERENYQQSSQSSQAKSTVPSFSINDKFTLNKEDASYSLVLEVRTAIDNVLIQSDVPIDLLDVDKNSAVVSFSSCDTESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQVRQYHIKPLSLHQRTHFIDHDRPMNTLTLTGQFSFAEVHSWVVFCLPEVPEKPPAGECATFYFQNTFLDTQLECVY
IRESfinder result
IRESfinder score
0.630436749
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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