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mmu_circBbs5_295
Basic information of circular RNA
PanCircBase ID:
mmu_circBbs5_295
mm39_Chr. Position ID:
chr2|69485842|69491442|+
Gene name:
Bbs5
Transcript:
ENSMUST00000134659.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,6,7
Exon/intron offsets:
0,1398,5537
Genomic length:
5600
Exon/intron sizes in nt:
136,96,63
Flanking exon/introns:
chr2:69484456-69485842|chr2:69491442-69493369
Splice length:
295
Other database IDs:
CIRCpedia: MMU_CIRCpedia_27232, circAtlas: mmu-Bbs5_0007
Human Ortholog IDs:
Mature spliced Sequence:
AGCATATGAAACTTCTAAAATGTACCGTGACTTTAAGTTGAGAAGTGCAGTCATTCAGAACAAGCAGCTGAGGTTACTACCACAGGAGCATGTGTATGATAAGATCAATGGCGTATGGAATCTGTCCAGCGACCAGGGGAATTTAGGAACTTTTTTTATTACCAATGTGAGAATTGTGTGGCATGCCAATATGAATGACAGTTTTAATGTCAGCATACCATATCTGCAAATTCGTTCAATAAAGATCAGAGATTCAAAATTTGGTTTGGCGCTTGTCATAGAAAGCTCTCAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAGCATACCATATCTGCA
56.88
ACCTCAGCTGCTTGTTCTGA
59.24
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTCAGCATACCATATCTGCAA
57.58
ACCTCAGCTGCTTGTTCTGA
59.24
162
PCR template sequence:
TGACAGTTTTAATGTCAGCATACCATATCTGCAAATTCGTTCAATAAAGATCAGAGATTCAAAATTTGGTTTGGCGCTTGTCATAGAAAGCTCTCAGCAGAGCATATGAAACTTCTAAAATGTACCGTGACTTTAAGTTGAGAAGTGCAGTCATTCAGAACAAGCAGCTGAGGTTACTACCACAGGAGCATGTGTATGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AAGCTCTCAGCAGAGCATATGAAACT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCBBS5_295_ORF_1
0.9349
0.026622365
0.205408554
285
ORF sequence:
ATGTACCGTGACTTTAAGTTGAGAAGTGCAGTCATTCAGAACAAGCAGCTGAGGTTACTACCACAGGAGCATGTGTATGATAAGATCAATGGCGTATGGAATCTGTCCAGCGACCAGGGGAATTTAGGAACTTTTTTTATTACCAATGTGAGAATTGTGTGGCATGCCAATATGAATGACAGTTTTAATGTCAGCATACCATATCTGCAAATTCGTTCAATAAAGATCAGAGATTCAAAATTTGGTTTGGCGCTTGTCATAGAAAGCTCTCAGCAGAGCATATGA
Peptide sequence:
MYRDFKLRSAVIQNKQLRLLPQEHVYDKINGVWNLSSDQGNLGTFFITNVRIVWHANMNDSFNVSIPYLQIRSIKIRDSKFGLALVIESSQQSI
IRESfinder result
IRESfinder score
0.670527678
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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