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mmu_circBaz1b_2164

Basic information of circular RNA
PanCircBase ID:

mmu_circBaz1b_2164

mm39_Chr. Position ID:

chr5|135237896|135250950|+

Gene name:

Baz1b

Transcript:

ENSMUST00000002825.6

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

4

Exon/intron indexes:

5,6,7,8

Exon/intron offsets:

0,1698,7547,12915

Genomic length:

13054

Exon/intron sizes in nt:

12,21,98,17,05,139

Flanking exon/introns:

chr5:135234045-135237896|chr5:135250950-135251938

Splice length:

2164

Other database IDs:

CIRCpedia: MMU_CIRCpedia_8224, circAtlas: mmu-Baz1b_0004

circAtlas: hsa-BAZ1B_0003, CIRCpedia: HSA_CIRCpedia_152783

Human Ortholog IDs:
Mature spliced Sequence:

ATGACAGAGCGCGTAGGTCTCCACGAAAACTTCCTACTTCATTAAAAAAAGGAGAAAGAAAATGGGCTCCTCCAAAATTTCTGCCTCACAAATATGATGTGAAGCTACAAAATGAAGATAAGATCATCAGCAATGTGCCAGCAGACAGCTTGATTCGAACGGAGAGACCACCAAATAAGGAGATACTTCGATACTTTATTCGGCATAATGCCTTACGTGCTGGTACTGGTGAGAATGCACCTTGGGTGGTGGAAGATGAATTGGTGAAGAAATATTCCCTGCCTAGCAAGTTCAGTGACTTTTTACTTGACCCATATAAGTATATGACTCTCAATCCCTCTACCAAGAGGAGGAATACTGGATCTCCAGACAGGAAGCCCTCAAAGAAACCTAAGAGAGACAGTTCTTCTCTGAGTTCCCCACTAAACCCTAAGCTGTGGTGTCATGTGCACTTGGAGAAGTCGTTAAATGGCCCACCACTCAAAGTAAAGAACTCAAAGAATTCCAAGTCTCCGGAAGAACATTTGGAAGGGGTGATGAAAATTATGTCCCCCAACAACAACAAGCTACACAGCTTCCACATTCCTAAGAAAGGCCCAGCCGCCAAGAAGCCAGGGAAGCACAGTGACAAACCTCTGAAGGCCAAGGGCAGAGGCAAAGGCATCCTGAATGGACAGAAGTCCACAGGCAACTCCAAATCACCCAGCAAGTGTGTGAAGACTCCTAAGACCAAAATGAAGCAGATGACTTTGTTGGATATGGCCAAAGGGACTCAGAAGATGACCCGCACCCCACGGAGTTCTGGGGGTGTGCCAAGGTCTTCTGGTAAACCTCACAAACACCTGCCTCCTGCAGCTCTGCATCTGATTGCCTATTACAAAGAAAATAAAGACAAAGAGGACAAGAAGAGTGCTCTGTCCTGTGTTATCTCCAAAACAGCACGTCTCCTCTCCAATGAAGATAGAGCCCGTCTTCCAGAAGAGCTCCGGGCTCTTGTCCAGAAACGCTATGAGCTCCTGGAACATAAAAAGAGGTGGGCCTCTATGTCTGAAGAGCAACGGAAAGAATATTTGAAAAAGAAACGACAGGAGCTAAAGGAGAGGTTGCGGGAAAAAGCCAAAGAACGGAGAGAGAGAGAAATGCTGGAGAGGTTAGAAAAGCAGAAGCGATTTGAGGACCAGGAGTTAGGTGGCAGAAACCTCCCAGCATTCAGGCTGGTAGATACCCCTGAGGGGCTCCCCAACACTCTTTTTGGGGATGTGGCCCTGGTGGTGGAGTTCCTGAGCTGTTACTCTGGGTTGCTCTTGCCCGATGCTCAGTACCCTATCACTGCTGTGTCCCTAATGGAAGCCTTGAGTGCAGATAAAGGTGGTTTTTTGTACTTGAACAGAGTGCTGGTCATCCTGCTACAGACCTTACTTCAGGATGAAATTGCAGAAGACTATGGAGAACTGGGAATGAAGCTGTCAGAAATCCCCCTCACCCTGCATTCTGTCTCAGAGCTGGTCCGGCTCTGCTTGCGCAGGTGTGATGTCCAGGAGGACAGCGAGGGCTCAGAAACAGATGACAATAAAGATTCCACACCATTTGAGGATAATGAGGTGCAAGACGAATTTCTAGAGAAGCTGGAGACATCAGAATTCTTTGAGCTGACCTCAGAGGAGAAGCTACGGATATTGACAGCTCTTTGCCACCGTATCCTCATGACATACTCTGTGCAAGACCACATGGAGACAAGACAGCAGGTGTCTGCAGAGTTGTGGAAGGAGCGGCTTGCTGTGCTGAAGGAGGAGAATGATAAGAAGAGAGCAGAGAAGCAAAAGCGGAAGGAGATGGAAGCCAGAAATAAAGAAAATGGCAAAGAGGAGAATGTGCTAGGCAAAGTGGACAGGAAAAAAGAAATTGTGAAGATTGAACAGCAAGTAGAAGTGGAAGCAGATGACATGATCAGTGCTGTCAAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAACAAAAGTGAGATTGGAACGGGAAGCTGAAGAGGAACGAATGCGGAAACACAAGGCTGCTGCCGAGAAGGCTTTCCAGGAAGGGATTGCCAAGGCAAAACTTGTACTGCGCAGGACACCTATTGGTACAGACCGCAACCATAACAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

AGGCAAAACTTGTACTGCGC

59.69

GGCAGAAATTTTGGAGGAGCC

59.79

140

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

CCGAGAAGGCTTTCCAGGAA

59.68

GCAGAAATTTTGGAGGAGCCC

59.79

169

PCR template sequence:

AACACAAGGCTGCTGCCGAGAAGGCTTTCCAGGAAGGGATTGCCAAGGCAAAACTTGTACTGCGCAGGACACCTATTGGTACAGACCGCAACCATAACAGATGACAGAGCGCGTAGGTCTCCACGAAAACTTCCTACTTCATTAAAAAAAGGAGAAAGAAAATGGGCTCCTCCAAAATTTCTGCCTCACAAATATGATGT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

17

Interacting miRNAs (#binding sites)

mmu-miR-1a-3p(1), mmu-miR-7b-5p(1), mmu-miR-206-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-335-5p(1), mmu-miR-18a-5p(1), mmu-miR-431-5p(1), mmu-miR-194-5p(1), mmu-miR-98-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-3065-5p(1), mmu-miR-1199-5p(1), mmu-miR-1904(1)

siRNAs for circular RNA silencing
Junction sequence

GCAACCATAACAGATGACAGAGCGCG

siRNA sets

CTCTGTCATCTGTTATGGT,GCTCTGTCATCTGTTATGG

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBAZ1B_2164_ORF_1

1.1732

0.315467012

0.999989259

1845

ORF sequence:

ATGACTCTCAATCCCTCTACCAAGAGGAGGAATACTGGATCTCCAGACAGGAAGCCCTCAAAGAAACCTAAGAGAGACAGTTCTTCTCTGAGTTCCCCACTAAACCCTAAGCTGTGGTGTCATGTGCACTTGGAGAAGTCGTTAAATGGCCCACCACTCAAAGTAAAGAACTCAAAGAATTCCAAGTCTCCGGAAGAACATTTGGAAGGGGTGATGAAAATTATGTCCCCCAACAACAACAAGCTACACAGCTTCCACATTCCTAAGAAAGGCCCAGCCGCCAAGAAGCCAGGGAAGCACAGTGACAAACCTCTGAAGGCCAAGGGCAGAGGCAAAGGCATCCTGAATGGACAGAAGTCCACAGGCAACTCCAAATCACCCAGCAAGTGTGTGAAGACTCCTAAGACCAAAATGAAGCAGATGACTTTGTTGGATATGGCCAAAGGGACTCAGAAGATGACCCGCACCCCACGGAGTTCTGGGGGTGTGCCAAGGTCTTCTGGTAAACCTCACAAACACCTGCCTCCTGCAGCTCTGCATCTGATTGCCTATTACAAAGAAAATAAAGACAAAGAGGACAAGAAGAGTGCTCTGTCCTGTGTTATCTCCAAAACAGCACGTCTCCTCTCCAATGAAGATAGAGCCCGTCTTCCAGAAGAGCTCCGGGCTCTTGTCCAGAAACGCTATGAGCTCCTGGAACATAAAAAGAGGTGGGCCTCTATGTCTGAAGAGCAACGGAAAGAATATTTGAAAAAGAAACGACAGGAGCTAAAGGAGAGGTTGCGGGAAAAAGCCAAAGAACGGAGAGAGAGAGAAATGCTGGAGAGGTTAGAAAAGCAGAAGCGATTTGAGGACCAGGAGTTAGGTGGCAGAAACCTCCCAGCATTCAGGCTGGTAGATACCCCTGAGGGGCTCCCCAACACTCTTTTTGGGGATGTGGCCCTGGTGGTGGAGTTCCTGAGCTGTTACTCTGGGTTGCTCTTGCCCGATGCTCAGTACCCTATCACTGCTGTGTCCCTAATGGAAGCCTTGAGTGCAGATAAAGGTGGTTTTTTGTACTTGAACAGAGTGCTGGTCATCCTGCTACAGACCTTACTTCAGGATGAAATTGCAGAAGACTATGGAGAACTGGGAATGAAGCTGTCAGAAATCCCCCTCACCCTGCATTCTGTCTCAGAGCTGGTCCGGCTCTGCTTGCGCAGGTGTGATGTCCAGGAGGACAGCGAGGGCTCAGAAACAGATGACAATAAAGATTCCACACCATTTGAGGATAATGAGGTGCAAGACGAATTTCTAGAGAAGCTGGAGACATCAGAATTCTTTGAGCTGACCTCAGAGGAGAAGCTACGGATATTGACAGCTCTTTGCCACCGTATCCTCATGACATACTCTGTGCAAGACCACATGGAGACAAGACAGCAGGTGTCTGCAGAGTTGTGGAAGGAGCGGCTTGCTGTGCTGAAGGAGGAGAATGATAAGAAGAGAGCAGAGAAGCAAAAGCGGAAGGAGATGGAAGCCAGAAATAAAGAAAATGGCAAAGAGGAGAATGTGCTAGGCAAAGTGGACAGGAAAAAAGAAATTGTGAAGATTGAACAGCAAGTAGAAGTGGAAGCAGATGACATGATCAGTGCTGTCAAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAACAAAAGTGAGATTGGAACGGGAAGCTGAAGAGGAACGAATGCGGAAACACAAGGCTGCTGCCGAGAAGGCTTTCCAGGAAGGGATTGCCAAGGCAAAACTTGTACTGCGCAGGACACCTATTGGTACAGACCGCAACCATAACAGATGA

Peptide sequence:

MTLNPSTKRRNTGSPDRKPSKKPKRDSSSLSSPLNPKLWCHVHLEKSLNGPPLKVKNSKNSKSPEEHLEGVMKIMSPNNNKLHSFHIPKKGPAAKKPGKHSDKPLKAKGRGKGILNGQKSTGNSKSPSKCVKTPKTKMKQMTLLDMAKGTQKMTRTPRSSGGVPRSSGKPHKHLPPAALHLIAYYKENKDKEDKKSALSCVISKTARLLSNEDRARLPEELRALVQKRYELLEHKKRWASMSEEQRKEYLKKKRQELKERLREKAKERREREMLERLEKQKRFEDQELGGRNLPAFRLVDTPEGLPNTLFGDVALVVEFLSCYSGLLLPDAQYPITAVSLMEALSADKGGFLYLNRVLVILLQTLLQDEIAEDYGELGMKLSEIPLTLHSVSELVRLCLRRCDVQEDSEGSETDDNKDSTPFEDNEVQDEFLEKLETSEFFELTSEEKLRILTALCHRILMTYSVQDHMETRQQVSAELWKERLAVLKEENDKKRAEKQKRKEMEARNKENGKEENVLGKVDRKKEIVKIEQQVEVEADDMISAVKSRRLLSMQAKRKREIQERETKVRLEREAEEERMRKHKAAAEKAFQEGIAKAKLVLRRTPIGTDRNHNR

IRESfinder result

IRESfinder score

0.354220531

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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