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mmu_circBach1_1845

Basic information of circular RNA
PanCircBase ID:

mmu_circBach1_1845

mm39_Chr. Position ID:

chr16|87512218|87519496|+

Gene name:

Bach1

Transcript:

ENSMUST00000026703.6

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

3

Exon/intron indexes:

2,3,4

Exon/intron offsets:

0,3476,7071

Genomic length:

7278

Exon/intron sizes in nt:

294,1344,207

Flanking exon/introns:

chr16:87495944-87512218|chr16:87519496-87526324

Splice length:

1845

Other database IDs:

circBase: mmu_circ_0006436, CIRCpedia: MMU_CIRCpedia_32902, circAtlas: mmu-Bach1_0004

circBase: hsa_circ_0001181, circAtlas: hsa-BACH1_0001, CIRCpedia: HSA_CIRCpedia_35162

Human Ortholog IDs:
Mature spliced Sequence:

GTCGATGACAGTGAGAAGCATGCTTTCCACTGCTCTCCCTGGTCCCAGTTGCCACCCAGGATGTCTGTGAGTGAGAGTGCGGTATTTGCCTACGAGTCCTCTGTGCATAGCACCAACGTCTTGCTCAGCCTCAATGACCAGCGGAAGAAGGATGTCCTGTGTGATGTGACTGTCCTGGTGGAGGGCCAGCGGTTCCGAGCCCACCGCTCGGTGCTGGCTGCGTGCAGCAGCTACTTCCACTCGAGAATCGTAGGCCAGACTGACGCAGAGCTCACCGTCACACTGCCTGAAGAGGTAACGGTTAAAGGATTTGAACCTTTAATTCAGTTTGCCTACACTGCCAAACTCATTTTAAGTAAAGACAATGTTGACGAAGTGTGCAGGTGTGTGGAGTTTCTAAGCGTACACAATATCGAGGAATCCTGCTTCCAGTTTCTCAAGTTTAAGTTTTTGGACTCCACTTCAGAGCAGCAGGAATGCGCAAGAAAAAAATGCTTCTCCTCACACTGTCAGAAAGCAGATTTTAAATTTTCATTTTCAGAACAGAAAGATCTCGAAATCGATGAAGCAGATGAATTCTTGGAAAAGAAACGTGTTCAGACGCCTCAGTGTGACTCCCGCAGGTGTCAGGGCAGTGTAAAAGCATCCCCCCCTCTCCAGGACAGTGTCAGTCAGGCGTGCCAGTCCCTGTGCACGGACAAGGATGGAGCCCTGGCATTGCCATCTCTATGCCCCAAATACAGAAAGTTCCAGAAAGCGTTTGGAACTGACAAGATCCGAACTCTAGAATCCGGTGTCAGAGATGTCCACACTGCCTCTGTCCAGCCAAATGAGACCTCTGAACTTGAGTGTTTTGGGGGAGCGCAGGGCTGTGCAGATTTACACGTGATTTTAAAATGTGAAGGAATGAAGGCAGCCATGGAGAGTGAAGACACAGAGGGCCAGGATCCCTCCCCTCAGTGCCCTGCAGAGCAGCCCCAAGGGACACCCTTGCCTCAGGATTCTGCAGGACCTCACGGGCTCTACTCCTTGTCAGCCTTACACACATATGAGCAGTCAGGTGACGTGGCCTTTGCTGGGGTGCAGAGTAAAACCGTGAAGACAGAAAAGCCTCTGTCGAGGCCAGATGCCCAGGACGAGAAGCCATCGGAAAATCAGGATTTATATCTGAAGTCTAGCATGGGCCCTAAAGAAGACAGCAGCAGCCTTGCATCTGAGGATCGGAGTAGTGTGGAGCGAGAGGTGGCAGAGCACCTGGCCAAAGGCTTCTGGAGTGACATTTGCAGCACGGACTCGCCTTGCCAAATGCAGTTGTCGCCCACTGTGGCCAAAGACGGCCCAGAACAGGGCTACTCGCAAAGGCGATCTGAGTGTCCCTGGTTGGGTATCAGGATCAGTGAGAGCCCCGAGCCAGGCCAGCGGACTTTCACAACTCTCAGTTCCGTCAACTGCCCTTTTATCAGTACTCTGAGTTCCGAAGGCTGCTCAAGCAACTTGGAAATTGGAAACTACGATTATGTCTCGGAGCCTCAGCAGGAGCCTTGCCCGTATGCTTGTGTGATTAGCCTGGGAGATGACTCTGAGACGGACACGGAAGGTGACAGCGAGTCCTGTTCTGCCAGGGAGCAGGACTGTGAGGTGAAGCTGCCATTCAATGCCCAACGGATAATTTCGCTCTCACGAAATGATTTCCAATCCTTGTTGAAAATGCACAAACTGACCCCAGAGCAGCTCGACTGTATCCATGACATCCGCAGAAGGAGTAAGAACAGAATCGCCGCGCAACGCTGTCGCAAGAGGAAACTTGACTGTATCCAGAACCTTGAGTCGGAAATCGAGAAGCTG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GTAAGAACAGAATCGCCGCG

59.7

CTCACAGACATCCTGGGTGG

59.75

154

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GTAAGAACAGAATCGCCGCG

59.7

CGCACTCTCACTCACAGACA

59.69

164

PCR template sequence:

TGACATCCGCAGAAGGAGTAAGAACAGAATCGCCGCGCAACGCTGTCGCAAGAGGAAACTTGACTGTATCCAGAACCTTGAGTCGGAAATCGAGAAGCTGGTCGATGACAGTGAGAAGCATGCTTTCCACTGCTCTCCCTGGTCCCAGTTGCCACCCAGGATGTCTGTGAGTGAGAGTGCGGTATTTGCCTACGAGTCCT

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

20

Interacting miRNAs (#binding sites)

mmu-miR-34c-5p(3), mmu-miR-34b-5p(3), mmu-miR-223-3p(2), mmu-miR-149-5p(2), mmu-miR-680(2), mmu-miR-697(2), mmu-miR-1904(2), mmu-miR-135a-5p(1), mmu-miR-34a-5p(1), mmu-miR-205-5p(1), mmu-miR-328-3p(1), mmu-miR-433-5p(1), mmu-miR-370-3p(1), mmu-miR-690(1), mmu-miR-377-3p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-300-3p(1), mmu-miR-673-5p(1), mmu-miR-323-3p(1)

siRNAs for circular RNA silencing
Junction sequence

AATCGAGAAGCTGGTCGATGACAGTG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCBACH1_1845_ORF_1

0.8176

0.279156417

1

3630

ORF sequence:

ATGTCTGTGAGTGAGAGTGCGGTATTTGCCTACGAGTCCTCTGTGCATAGCACCAACGTCTTGCTCAGCCTCAATGACCAGCGGAAGAAGGATGTCCTGTGTGATGTGACTGTCCTGGTGGAGGGCCAGCGGTTCCGAGCCCACCGCTCGGTGCTGGCTGCGTGCAGCAGCTACTTCCACTCGAGAATCGTAGGCCAGACTGACGCAGAGCTCACCGTCACACTGCCTGAAGAGGTAACGGTTAAAGGATTTGAACCTTTAATTCAGTTTGCCTACACTGCCAAACTCATTTTAAGTAAAGACAATGTTGACGAAGTGTGCAGGTGTGTGGAGTTTCTAAGCGTACACAATATCGAGGAATCCTGCTTCCAGTTTCTCAAGTTTAAGTTTTTGGACTCCACTTCAGAGCAGCAGGAATGCGCAAGAAAAAAATGCTTCTCCTCACACTGTCAGAAAGCAGATTTTAAATTTTCATTTTCAGAACAGAAAGATCTCGAAATCGATGAAGCAGATGAATTCTTGGAAAAGAAACGTGTTCAGACGCCTCAGTGTGACTCCCGCAGGTGTCAGGGCAGTGTAAAAGCATCCCCCCCTCTCCAGGACAGTGTCAGTCAGGCGTGCCAGTCCCTGTGCACGGACAAGGATGGAGCCCTGGCATTGCCATCTCTATGCCCCAAATACAGAAAGTTCCAGAAAGCGTTTGGAACTGACAAGATCCGAACTCTAGAATCCGGTGTCAGAGATGTCCACACTGCCTCTGTCCAGCCAAATGAGACCTCTGAACTTGAGTGTTTTGGGGGAGCGCAGGGCTGTGCAGATTTACACGTGATTTTAAAATGTGAAGGAATGAAGGCAGCCATGGAGAGTGAAGACACAGAGGGCCAGGATCCCTCCCCTCAGTGCCCTGCAGAGCAGCCCCAAGGGACACCCTTGCCTCAGGATTCTGCAGGACCTCACGGGCTCTACTCCTTGTCAGCCTTACACACATATGAGCAGTCAGGTGACGTGGCCTTTGCTGGGGTGCAGAGTAAAACCGTGAAGACAGAAAAGCCTCTGTCGAGGCCAGATGCCCAGGACGAGAAGCCATCGGAAAATCAGGATTTATATCTGAAGTCTAGCATGGGCCCTAAAGAAGACAGCAGCAGCCTTGCATCTGAGGATCGGAGTAGTGTGGAGCGAGAGGTGGCAGAGCACCTGGCCAAAGGCTTCTGGAGTGACATTTGCAGCACGGACTCGCCTTGCCAAATGCAGTTGTCGCCCACTGTGGCCAAAGACGGCCCAGAACAGGGCTACTCGCAAAGGCGATCTGAGTGTCCCTGGTTGGGTATCAGGATCAGTGAGAGCCCCGAGCCAGGCCAGCGGACTTTCACAACTCTCAGTTCCGTCAACTGCCCTTTTATCAGTACTCTGAGTTCCGAAGGCTGCTCAAGCAACTTGGAAATTGGAAACTACGATTATGTCTCGGAGCCTCAGCAGGAGCCTTGCCCGTATGCTTGTGTGATTAGCCTGGGAGATGACTCTGAGACGGACACGGAAGGTGACAGCGAGTCCTGTTCTGCCAGGGAGCAGGACTGTGAGGTGAAGCTGCCATTCAATGCCCAACGGATAATTTCGCTCTCACGAAATGATTTCCAATCCTTGTTGAAAATGCACAAACTGACCCCAGAGCAGCTCGACTGTATCCATGACATCCGCAGAAGGAGTAAGAACAGAATCGCCGCGCAACGCTGTCGCAAGAGGAAACTTGACTGTATCCAGAACCTTGAGTCGGAAATCGAGAAGCTGGTCGATGACAGTGAGAAGCATGCTTTCCACTGCTCTCCCTGGTCCCAGTTGCCACCCAGGATGTCTGTGAGTGAGAGTGCGGTATTTGCCTACGAGTCCTCTGTGCATAGCACCAACGTCTTGCTCAGCCTCAATGACCAGCGGAAGAAGGATGTCCTGTGTGATGTGACTGTCCTGGTGGAGGGCCAGCGGTTCCGAGCCCACCGCTCGGTGCTGGCTGCGTGCAGCAGCTACTTCCACTCGAGAATCGTAGGCCAGACTGACGCAGAGCTCACCGTCACACTGCCTGAAGAGGTAACGGTTAAAGGATTTGAACCTTTAATTCAGTTTGCCTACACTGCCAAACTCATTTTAAGTAAAGACAATGTTGACGAAGTGTGCAGGTGTGTGGAGTTTCTAAGCGTACACAATATCGAGGAATCCTGCTTCCAGTTTCTCAAGTTTAAGTTTTTGGACTCCACTTCAGAGCAGCAGGAATGCGCAAGAAAAAAATGCTTCTCCTCACACTGTCAGAAAGCAGATTTTAAATTTTCATTTTCAGAACAGAAAGATCTCGAAATCGATGAAGCAGATGAATTCTTGGAAAAGAAACGTGTTCAGACGCCTCAGTGTGACTCCCGCAGGTGTCAGGGCAGTGTAAAAGCATCCCCCCCTCTCCAGGACAGTGTCAGTCAGGCGTGCCAGTCCCTGTGCACGGACAAGGATGGAGCCCTGGCATTGCCATCTCTATGCCCCAAATACAGAAAGTTCCAGAAAGCGTTTGGAACTGACAAGATCCGAACTCTAGAATCCGGTGTCAGAGATGTCCACACTGCCTCTGTCCAGCCAAATGAGACCTCTGAACTTGAGTGTTTTGGGGGAGCGCAGGGCTGTGCAGATTTACACGTGATTTTAAAATGTGAAGGAATGAAGGCAGCCATGGAGAGTGAAGACACAGAGGGCCAGGATCCCTCCCCTCAGTGCCCTGCAGAGCAGCCCCAAGGGACACCCTTGCCTCAGGATTCTGCAGGACCTCACGGGCTCTACTCCTTGTCAGCCTTACACACATATGAGCAGTCAGGTGACGTGGCCTTTGCTGGGGTGCAGAGTAAAACCGTGAAGACAGAAAAGCCTCTGTCGAGGCCAGATGCCCAGGACGAGAAGCCATCGGAAAATCAGGATTTATATCTGAAGTCTAGCATGGGCCCTAAAGAAGACAGCAGCAGCCTTGCATCTGAGGATCGGAGTAGTGTGGAGCGAGAGGTGGCAGAGCACCTGGCCAAAGGCTTCTGGAGTGACATTTGCAGCACGGACTCGCCTTGCCAAATGCAGTTGTCGCCCACTGTGGCCAAAGACGGCCCAGAACAGGGCTACTCGCAAAGGCGATCTGAGTGTCCCTGGTTGGGTATCAGGATCAGTGAGAGCCCCGAGCCAGGCCAGCGGACTTTCACAACTCTCAGTTCCGTCAACTGCCCTTTTATCAGTACTCTGAGTTCCGAAGGCTGCTCAAGCAACTTGGAAATTGGAAACTACGATTATGTCTCGGAGCCTCAGCAGGAGCCTTGCCCGTATGCTTGTGTGATTAGCCTGGGAGATGACTCTGAGACGGACACGGAAGGTGACAGCGAGTCCTGTTCTGCCAGGGAGCAGGACTGTGAGGTGAAGCTGCCATTCAATGCCCAACGGATAATTTCGCTCTCACGAAATGATTTCCAATCCTTGTTGAAAATGCACAAACTGACCCCAGAGCAGCTCGACTGTATCCATGACATCCGCAGAAGGAGTAAGAACAGAATCGCCGCGCAACGCTGTCGCAAGAGGAAACTTGACTGTATCCAGAACCTTGAGTCGGAAATCGAGAAGCTG

Peptide sequence:

MSVSESAVFAYESSVHSTNVLLSLNDQRKKDVLCDVTVLVEGQRFRAHRSVLAACSSYFHSRIVGQTDAELTVTLPEEVTVKGFEPLIQFAYTAKLILSKDNVDEVCRCVEFLSVHNIEESCFQFLKFKFLDSTSEQQECARKKCFSSHCQKADFKFSFSEQKDLEIDEADEFLEKKRVQTPQCDSRRCQGSVKASPPLQDSVSQACQSLCTDKDGALALPSLCPKYRKFQKAFGTDKIRTLESGVRDVHTASVQPNETSELECFGGAQGCADLHVILKCEGMKAAMESEDTEGQDPSPQCPAEQPQGTPLPQDSAGPHGLYSLSALHTYEQSGDVAFAGVQSKTVKTEKPLSRPDAQDEKPSENQDLYLKSSMGPKEDSSSLASEDRSSVEREVAEHLAKGFWSDICSTDSPCQMQLSPTVAKDGPEQGYSQRRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSSEGCSSNLEIGNYDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQDCEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLVDDSEKHAFHCSPWSQLPPRMSVSESAVFAYESSVHSTNVLLSLNDQRKKDVLCDVTVLVEGQRFRAHRSVLAACSSYFHSRIVGQTDAELTVTLPEEVTVKGFEPLIQFAYTAKLILSKDNVDEVCRCVEFLSVHNIEESCFQFLKFKFLDSTSEQQECARKKCFSSHCQKADFKFSFSEQKDLEIDEADEFLEKKRVQTPQCDSRRCQGSVKASPPLQDSVSQACQSLCTDKDGALALPSLCPKYRKFQKAFGTDKIRTLESGVRDVHTASVQPNETSELECFGGAQGCADLHVILKCEGMKAAMESEDTEGQDPSPQCPAEQPQGTPLPQDSAGPHGLYSLSALHTYEQSGDVAFAGVQSKTVKTEKPLSRPDAQDEKPSENQDLYLKSSMGPKEDSSSLASEDRSSVEREVAEHLAKGFWSDICSTDSPCQMQLSPTVAKDGPEQGYSQRRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSSEGCSSNLEIGNYDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQDCEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKL

IRESfinder result

IRESfinder score

0.457436349

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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