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mmu_circAtrx_255

Basic information of circular RNA
PanCircBase ID:

mmu_circAtrx_255

mm39_Chr. Position ID:

chrX|104954179|104955260|-

Gene name:

Atrx

Transcript:

ENSMUST00000150914.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

2

Exon/intron indexes:

3,2

Exon/intron offsets:

0,896

Genomic length:

1081

Exon/intron sizes in nt:

70,185

Flanking exon/introns:

chrX:104943599-104954179|chrX:104955260-104972564

Splice length:

255

Other database IDs:

CIRCpedia: MMU_CIRCpedia_1638, circAtlas: mmu-Atrx_0054

Human Ortholog IDs:
Mature spliced Sequence:

TTGTGAGCGGCCATTGAGTTCTAGGAATCGAACCCGGGTCCTCTGGAAGAGCAACCAGTGCTCTTAACCGCTGAACCATCTCTCCAGCTGGCGACAGCTGCTGCAGCAGGGGTTTTAACTCTTCACTCAATCCGTCCTTGGCTTCCAGCAGCTTGTCAGAAGCTATTTTAAGTACTCTGTTTCAGTTTCAGGGTATCCAGCACCTTCTTCTGGCTTCTCTGGATATCAGGCATGCACACTTCAGACAAAACGCAT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

ATCCAGCACCTTCTTCTGGC

60.03

CTGGAGAGATGGTTCAGCGG

60.18

148

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

ATCCAGCACCTTCTTCTGGC

60.03

GGAGAGATGGTTCAGCGGTT

59.75

146

PCR template sequence:

TCAGAAGCTATTTTAAGTACTCTGTTTCAGTTTCAGGGTATCCAGCACCTTCTTCTGGCTTCTCTGGATATCAGGCATGCACACTTCAGACAAAACGCATTTGTGAGCGGCCATTGAGTTCTAGGAATCGAACCCGGGTCCTCTGGAAGAGCAACCAGTGCTCTTAACCGCTGAACCATCTCTCCAGCTGGCGACAGCTG

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

3

Interacting miRNAs (#binding sites)

mmu-miR-370-3p(1), mmu-miR-384-3p(1), mmu-miR-574-5p(1)

siRNAs for circular RNA silencing
Junction sequence

AGACAAAACGCATTTGTGAGCGGCCA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

NA

NA

NA

NA

NA

ORF sequence:

NA

Peptide sequence:

NA

IRESfinder result

IRESfinder score

0.787173143

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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