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mmu_circAsh1l_324
Basic information of circular RNA
PanCircBase ID:
mmu_circAsh1l_324
mm39_Chr. Position ID:
chr3|88933718|88942788|+
Gene name:
Ash1l
Transcript:
ENSMUST00000186583.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
7,8,9,10
Exon/intron offsets:
0,6847,8805,8961
Genomic length:
9070
Exon/intron sizes in nt:
95,74,46,109
Flanking exon/introns:
chr3:88930517-88933718|chr3:88942788-88950425
Splice length:
324
Other database IDs:
CIRCpedia: MMU_CIRCpedia_33288, circAtlas: mmu-Ash1l_0025
circBase: hsa_circ_0110877, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_109648
Human Ortholog IDs:
Mature spliced Sequence:
CCCAAAGAGCCGATTAATCCAATTAAAGAAAGAAAAGTTGGAGTATACTCCAGGAGAGCACGAGTATGGGTTGTTCCCAGCGCCCATTCATGTTGGAAAGTATCTCAGACAGAAGAGAATTGACTTCCAGCTTCCCTATGATATTCTCTGGCAGTGGAAACACAATCAGCTATACAAAAAGCCAGATGTCCCCCTGTATAAAAAAATCCGTTCAAATGTCTATGTTGATGTCAAACCACTTTCTGGTTATGAGGCTACCACCTGTAATTGTAAGAAGCCAGATGACGACACCAGGAAGGGCTGTGGGGATGACTGCCTAAATAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AAGCCAGATGACGACACCAG
60.04
TTTCCAACATGAATGGGCGC
59.75
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGCCAGATGACGACACCA
60.04
TTTCCAACATGAATGGGCGC
59.75
150
PCR template sequence:
TTGATGTCAAACCACTTTCTGGTTATGAGGCTACCACCTGTAATTGTAAGAAGCCAGATGACGACACCAGGAAGGGCTGTGGGGATGACTGCCTAAATAGCCCAAAGAGCCGATTAATCCAATTAAAGAAAGAAAAGTTGGAGTATACTCCAGGAGAGCACGAGTATGGGTTGTTCCCAGCGCCCATTCATGTTGGAAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-138-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTGCCTAAATAGCCCAAAGAGCCGA
siRNA sets
TTTGGGCTATTTAGGCAGT, CTCTTTGGGCTATTTAGGC, TCTTTGGGCTATTTAGGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.354672984
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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