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mmu_circArhgap32_519
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap32_519
mm39_Chr. Position ID:
chr9|32081385|32101357|+
Gene name:
Arhgap32
Transcript:
ENSMUST00000174641.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,11752,16239,17544,19894
Genomic length:
19972
Exon/intron sizes in nt:
87,138,93,123,78
Flanking exon/introns:
chr9:32064420-32081385|chr9:32101357-32119479
Splice length:
519
Other database IDs:
circBase: mmu_circ_0015524, CIRCpedia: MMU_CIRCpedia_16397, circAtlas: mmu-Arhgap32_0005
circBase: hsa_circ_0095336, circAtlas: hsa-ARHGAP32_0008, CIRCpedia: HSA_CIRCpedia_155684
Human Ortholog IDs:
Mature spliced Sequence:
CTCTCACTATCAGAAGAACAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATTGCCTGTCAGGGCAAGAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTCGACAAACATCTTCATCTCTGCATTTATGACCGCCGATTCTCCCAGCTCACAGAGCTTCCCCGCTCTGACGTCCTGAAGGACAGTCCTGAGTCGGTTACTCAGATGCTTACAGCTTACCTGTCACGCCTTTCAACTATCGCTGGCAACAAGATCAACTGTGGGCCTGCCCTTACCTGGATGGAGATCGACAATAAGGGAAACCATCTTCTAGTTCACGAGGAGTCGTCCATCAACACTCCTGCTGTCGGTGCTGCCCATGTTATCAAGCGCTACACCGCTCGGGCCCCTGATGAACTGACATTAGAGGTGGGAGACATCGTTTCTGTTATTGACATGCCTCCCAAAGTATTAAGCACATGGTGGAGAGGCAAGCATGGATTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGGGAGACATCGTTTCTGT
60
TCCAACTCTTGCCCTGACAG
59.6
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGGGAGACATCGTTTCTGT
60
CCAACTCTTGCCCTGACAGG
60.61
178
PCR template sequence:
CCCTGATGAACTGACATTAGAGGTGGGAGACATCGTTTCTGTTATTGACATGCCTCCCAAAGTATTAAGCACATGGTGGAGAGGCAAGCATGGATTTCAGCTCTCACTATCAGAAGAACAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATTGCCTGTCAGGGCAAGAGTTGGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-223-3p(1), mmu-let-7c-5p(1), mmu-miR-143-3p(1), mmu-miR-218-5p(1), mmu-miR-98-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GCATGGATTTCAGCTCTCACTATCAG
siRNA sets
ATAGTGAGAGCTGAAATCC,GATAGTGAGAGCTGAAATC,TGAGAGCTGAAATCCATGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP32_519_ORF_1
1.1436
0.25710687
0.994428906
1008
ORF sequence:
ATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATTGCCTGTCAGGGCAAGAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTCGACAAACATCTTCATCTCTGCATTTATGACCGCCGATTCTCCCAGCTCACAGAGCTTCCCCGCTCTGACGTCCTGAAGGACAGTCCTGAGTCGGTTACTCAGATGCTTACAGCTTACCTGTCACGCCTTTCAACTATCGCTGGCAACAAGATCAACTGTGGGCCTGCCCTTACCTGGATGGAGATCGACAATAAGGGAAACCATCTTCTAGTTCACGAGGAGTCGTCCATCAACACTCCTGCTGTCGGTGCTGCCCATGTTATCAAGCGCTACACCGCTCGGGCCCCTGATGAACTGACATTAGAGGTGGGAGACATCGTTTCTGTTATTGACATGCCTCCCAAAGTATTAAGCACATGGTGGAGAGGCAAGCATGGATTTCAGCTCTCACTATCAGAAGAACAGAATGAAGTAATGAAAAATGGCTGTGAATCTAAAGAGCTGGTCTACCTCGTGCAGATTGCCTGTCAGGGCAAGAGTTGGATTGTTAAAAGAAGTTATGAAGATTTTCGGGTACTCGACAAACATCTTCATCTCTGCATTTATGACCGCCGATTCTCCCAGCTCACAGAGCTTCCCCGCTCTGACGTCCTGAAGGACAGTCCTGAGTCGGTTACTCAGATGCTTACAGCTTACCTGTCACGCCTTTCAACTATCGCTGGCAACAAGATCAACTGTGGGCCTGCCCTTACCTGGATGGAGATCGACAATAAGGGAAACCATCTTCTAGTTCACGAGGAGTCGTCCATCAACACTCCTGCTGTCGGTGCTGCCCATGTTATCAAGCGCTACACCGCTCGGGCCCCTGATGAACTGACATTAGAGGTGGGAGACATCGTTTCTGTTATTGACATGCCTCCCAAAGTATTAAGCACATGGTGGAGAGGCAAGCATGGATTTCAG
Peptide sequence:
MKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLTELPRSDVLKDSPESVTQMLTAYLSRLSTIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWWRGKHGFQLSLSEEQNEVMKNGCESKELVYLVQIACQGKSWIVKRSYEDFRVLDKHLHLCIYDRRFSQLTELPRSDVLKDSPESVTQMLTAYLSRLSTIAGNKINCGPALTWMEIDNKGNHLLVHEESSINTPAVGAAHVIKRYTARAPDELTLEVGDIVSVIDMPPKVLSTWWRGKHGFQ
IRESfinder result
IRESfinder score
0.46501496
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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