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mmu_circArhgap26_332
Basic information of circular RNA
PanCircBase ID:
mmu_circArhgap26_332
mm39_Chr. Position ID:
chr18|39230682|39243609|+
Gene name:
Arhgap26
Transcript:
ENSMUST00000097593.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
2,3,4,5
Exon/intron offsets:
0,1942,4539,12825
Genomic length:
12927
Exon/intron sizes in nt:
96,62,72,102
Flanking exon/introns:
chr18:39126682-39230682|chr18:39243609-39244842
Splice length:
332
Other database IDs:
circBase: hsa_circ_0128188, circAtlas: hsa-ARHGAP26_0023, CIRCpedia: HSA_CIRCpedia_50574
Human Ortholog IDs:
Mature spliced Sequence:
ATTTGTCTTCGGCAAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAGTGTATAGGAGATGCCGAAACAGATGATGAGATGTGTATAGCGAGATCTTTGCAAGAATTTGCTGCTGTTCTCAGGAATCTTGAAGACGAGCGGAGTCGAATGATCGAGAATGCCAGCGAGGTGCTCATCACACCCTTGGAGAAGTTTCGAAAGGAGCAGATTGGGGCCGCCAGGGAAGCCAAAAAGAAGTATGACAAAGAGACCGAGAAGTACTGCGGCACCTTAGAAAAACATTTGAATTTGTCTTCCAAAAAGAAGGAATCCCAGCTACAGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGAAGTACTGCGGCACCTT
60.04
TCATCTGTTTCGGCATCTCCT
59.17
154
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAGACCGAGAAGTACTGCGG
59.9
TCATCTGTTTCGGCATCTCCT
59.17
160
PCR template sequence:
AGCCAAAAAGAAGTATGACAAAGAGACCGAGAAGTACTGCGGCACCTTAGAAAAACATTTGAATTTGTCTTCCAAAAAGAAGGAATCCCAGCTACAGGAGATTTGTCTTCGGCAAAGCGGAAGTTTGCAGATTCCTTAAATGAATTTAAATTTCAGTGTATAGGAGATGCCGAAACAGATGATGAGATGTGTATAGCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-7b-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-18a-5p(1), mmu-miR-7a-5p(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCAGCTACAGGAGATTTGTCTTCGGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCARHGAP26_332_ORF_1
0.8507
0.300904846
0.403780112
285
ORF sequence:
ATGTGTATAGCGAGATCTTTGCAAGAATTTGCTGCTGTTCTCAGGAATCTTGAAGACGAGCGGAGTCGAATGATCGAGAATGCCAGCGAGGTGCTCATCACACCCTTGGAGAAGTTTCGAAAGGAGCAGATTGGGGCCGCCAGGGAAGCCAAAAAGAAGTATGACAAAGAGACCGAGAAGTACTGCGGCACCTTAGAAAAACATTTGAATTTGTCTTCCAAAAAGAAGGAATCCCAGCTACAGGAGATTTGTCTTCGGCAAAGCGGAAGTTTGCAGATTCCTTAA
Peptide sequence:
MCIARSLQEFAAVLRNLEDERSRMIENASEVLITPLEKFRKEQIGAAREAKKKYDKETEKYCGTLEKHLNLSSKKKESQLQEICLRQSGSLQIP
IRESfinder result
IRESfinder score
0.272923174
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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