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mmu_circAplp2_298
Basic information of circular RNA
PanCircBase ID:
mmu_circAplp2_298
mm39_Chr. Position ID:
chr9|31088313|31089374|-
Gene name:
Aplp2
Transcript:
ENSMUST00000217641.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,887
Genomic length:
1061
Exon/intron sizes in nt:
1,24,174
Flanking exon/introns:
chr9:31083342-31088313|chr9:31089374-31122918
Splice length:
298
Other database IDs:
CIRCpedia: MMU_CIRCpedia_16076, circAtlas: mmu-Aplp2_0007
circBase: hsa_circ_0000372, circAtlas: hsa-APLP2_0009, CIRCpedia: HSA_CIRCpedia_3740
Human Ortholog IDs:
Mature spliced Sequence:
GCTCTTGCAGCCAATGCTGGAACAGGCTTCGCTGTTGCTGAGCCTCAGATTGCGATGTTCTGTGGGAAGCTGAATATGCATGTGAACATTCAGACTGGCAAATGGGAGCCTGACCCAACAGGCACCAAGAGCTGCCTTGGAACAAAGGAGGAGGTTCTTCAGTACTGCCAGGAGATATATCCAGAGCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCGAAGGGACAAAAGGCAGTGCAAGAGTCACATTGTTATACCATTCAAGTGTCTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGGAAGCAAACCAGCCAGT
60.18
ATCTGAGGCTCAGCAACAGC
60.39
144
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGATGGAAGCAAACCAGCCA
60.18
ATCTGAGGCTCAGCAACAGC
60.39
146
PCR template sequence:
AATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCGAAGGGACAAAAGGCAGTGCAAGAGTCACATTGTTATACCATTCAAGTGTCTTGGCTCTTGCAGCCAATGCTGGAACAGGCTTCGCTGTTGCTGAGCCTCAGATTGCGATGTTCTGTGGGAAGCTGAATATGCATGTGAACATTCAGACTGGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-24-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCAAGTGTCTTGGCTCTTGCAGCCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAPLP2_298_ORF_1
0.7749
0.084829953
0.195833367
285
ORF sequence:
ATGTTCTGTGGGAAGCTGAATATGCATGTGAACATTCAGACTGGCAAATGGGAGCCTGACCCAACAGGCACCAAGAGCTGCCTTGGAACAAAGGAGGAGGTTCTTCAGTACTGCCAGGAGATATATCCAGAGCTGCAGATCACAAATGTGATGGAAGCAAACCAGCCAGTCAATATTGATAGTTGGTGCCGAAGGGACAAAAGGCAGTGCAAGAGTCACATTGTTATACCATTCAAGTGTCTTGGCTCTTGCAGCCAATGCTGGAACAGGCTTCGCTGTTGCTGA
Peptide sequence:
MFCGKLNMHVNIQTGKWEPDPTGTKSCLGTKEEVLQYCQEIYPELQITNVMEANQPVNIDSWCRRDKRQCKSHIVIPFKCLGSCSQCWNRLRCC
IRESfinder result
IRESfinder score
0.486163896
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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