top of page

mmu_circAnks1_774

Basic information of circular RNA
PanCircBase ID:

mmu_circAnks1_774

mm39_Chr. Position ID:

chr17|28204039|28207890|+

Gene name:

Anks1

Transcript:

ENSMUST00000114842.9

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

5

Exon/intron indexes:

5,6,7,8,9

Exon/intron offsets:

0,1186,1485,1679,3654

Genomic length:

3851

Exon/intron sizes in nt:

297,76,102,102,197

Flanking exon/introns:

chr17:28197463-28204039|chr17:28207890-28210281

Splice length:

774

Other database IDs:

CIRCpedia: MMU_CIRCpedia_36651, circAtlas: mmu-Anks1_0002

circBase: hsa_circ_0076080, circAtlas: hsa-ANKS1A_0004, CIRCpedia: HSA_CIRCpedia_54817

Human Ortholog IDs:
Mature spliced Sequence:

AACAACGACAATGAGACGGCCCTGCACTGCGCGGCCCAGTACGGCCACACGGAGGTCGTGAAGGCTCTGCTGGAGGAGCTCACGGACCCCACCATGCGCAACAACAAGTTTGAAACGCCCCTGGACTTGGCGGCGCTGTACGGGCGGCTGGAGGTGGTGAAGCTGCTGCTGGGTGCACACCCCAATCTCCTGAGCTGCAGCACTCGGAAACACACGCCCCTGCACTTGGCAGCAAGGAATGGCCACAAGGCCGTGGTGCAGGTCCTGCTGGACGCCGGCATGGATAGCAACTATCAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCT

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCCAGTGGACGATCATCAGA

59.54

CCTCCAGCAGAGCCTTCAC

60.08

157

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GGCCAGTGGACGATCATCAG

60.53

CCTCCAGCAGAGCCTTCAC

60.08

158

PCR template sequence:

CCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCTAACAACGACAATGAGACGGCCCTGCACTGCGCGGCCCAGTACGGCCACACGGAGGTCGTGAAGGCTCTGCTGGAGGAGCTCACGGACCCCACCATGCGCA

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

15

Interacting miRNAs (#binding sites)

mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-let-7c-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-125b-5p(1), mmu-let-7d-5p(1), mmu-miR-125a-5p(1), mmu-miR-98-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-207(1), mmu-miR-452-3p(1)

siRNAs for circular RNA silencing
Junction sequence

TGGAGTGAGGCCTAACAACGACAATG

siRNA sets

TGTCGTTGTTAGGCCTCAC, TTGTCGTTGTTAGGCCTCA, CATTGTCGTTGTTAGGCCT

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCANKS1_774_ORF_1

1.2474

0.48034747

0.999925462

1455

ORF sequence:

ATGCGCAACAACAAGTTTGAAACGCCCCTGGACTTGGCGGCGCTGTACGGGCGGCTGGAGGTGGTGAAGCTGCTGCTGGGTGCACACCCCAATCTCCTGAGCTGCAGCACTCGGAAACACACGCCCCTGCACTTGGCAGCAAGGAATGGCCACAAGGCCGTGGTGCAGGTCCTGCTGGACGCCGGCATGGATAGCAACTATCAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCTAACAACGACAATGAGACGGCCCTGCACTGCGCGGCCCAGTACGGCCACACGGAGGTCGTGAAGGCTCTGCTGGAGGAGCTCACGGACCCCACCATGCGCAACAACAAGTTTGAAACGCCCCTGGACTTGGCGGCGCTGTACGGGCGGCTGGAGGTGGTGAAGCTGCTGCTGGGTGCACACCCCAATCTCCTGAGCTGCAGCACTCGGAAACACACGCCCCTGCACTTGGCAGCAAGGAATGGCCACAAGGCCGTGGTGCAGGTCCTGCTGGACGCCGGCATGGATAGCAACTATCAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTGCAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTGTCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAGAAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCACCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACGAGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACGATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCT

Peptide sequence:

MRNNKFETPLDLAALYGRLEVVKLLLGAHPNLLSCSTRKHTPLHLAARNGHKAVVQVLLDAGMDSNYQTEMGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRDLPSQKSQQIAALIEDHMTGKRSVKEVDRTSTAQLPLLSNTDAIAPMSQGSMEKTVTELILHFDTHADEEGPYEALYNAVSCHSLDSTASGRSSDRDSMNKEAEATGTRAAGVRPNNDNETALHCAAQYGHTEVVKALLEELTDPTMRNNKFETPLDLAALYGRLEVVKLLLGAHPNLLSCSTRKHTPLHLAARNGHKAVVQVLLDAGMDSNYQTEMGSALHEAALFGKTDVVQILLAAGIDVNIKDNRGLTALDTVRDLPSQKSQQIAALIEDHMTGKRSVKEVDRTSTAQLPLLSNTDAIAPMSQGSMEKTVTELILHFDTHADEEGPYEALYNAVSCHSLDSTASGRSSDRDSMNKEAEATGTRAAGVRP

IRESfinder result

IRESfinder score

0.226382198

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

bottom of page