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mmu_circAnkrd44_632
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkrd44_632
mm39_Chr. Position ID:
chr1|54766648|54792127|-
Gene name:
Ankrd44
Transcript:
ENSMUST00000179030.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
15,14,13,12,11,10,9
Exon/intron offsets:
0,2244,5438,7506,8740,15824,25400
Genomic length:
25479
Exon/intron sizes in nt:
81,141,69,64,83,115,79
Flanking exon/introns:
chr1:54762284-54766648|chr1:54792127-54801419
Splice length:
632
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGTAAGGATGGCAAAAGTCCTCTGCACATGACAGCTGTCCATGGACGGTTCACGCGGTCACAAACCCTCATCCAGAATGGAGGTGAGATCGACTGTGTGGATAAGGATGGCAACACACCTCTCCATGTGGCTGCGAGACATGGTCACGAGCTCCTGATTAACACCCTAATCACTAGCGGAGCCGACACAGCCAAGTGTGGAATCCATAGCATGTTCCCCCTACATTTAGCCGCCCTAAATGCTCACTCTGACTGCTGCAGGAAGTTGTTATCATCTGGCTTTGAAATAGACACCCCAGATACGTTTGGAAGAACGTGCCTCCACGCCGCTGCTGCAGGAGGCAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGCCATTTCCACTGTATTAAAGCATTAGTGACCACCGGAGCCAACGTTAATGAAACAGACGACTGGGGACGGACAGCTCTGCACTACGCTGCTGCATCAGACATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTTGAAAGAGCCAGAGAAGTGA
59.61
CCACACAGTCGATCTCACCT
59.11
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGCCCATGACAATTCTGAAGA
57.56
CCACACAGTCGATCTCACCT
59.11
163
PCR template sequence:
CATCAGACATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTAGTAAGGATGGCAAAAGTCCTCTGCACATGACAGCTGTCCATGGACGGTTCACGCGGTCACAAACCCTCATCCAGAATGGAGGTGAGATCGACTGTGTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(2), mmu-miR-155-5p(1), mmu-miR-221-3p(1), mmu-miR-130a-3p(1), mmu-miR-298-5p(1), mmu-miR-503-5p(1), mmu-miR-136-5p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-758-3p(1), mmu-miR-301a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAGATGCTGCACTAGTAAGGATGGCA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKRD44_632_ORF_1
0.9525
0.144054774
0.842535
636
ORF sequence:
ATGACAGCTGTCCATGGACGGTTCACGCGGTCACAAACCCTCATCCAGAATGGAGGTGAGATCGACTGTGTGGATAAGGATGGCAACACACCTCTCCATGTGGCTGCGAGACATGGTCACGAGCTCCTGATTAACACCCTAATCACTAGCGGAGCCGACACAGCCAAGTGTGGAATCCATAGCATGTTCCCCCTACATTTAGCCGCCCTAAATGCTCACTCTGACTGCTGCAGGAAGTTGTTATCATCTGGCTTTGAAATAGACACCCCAGATACGTTTGGAAGAACGTGCCTCCACGCCGCTGCTGCAGGAGGCAATGTAGAATGCATAAAGCTCTTGCAGAGCAGCGGAGCAGATTTTCACAAAAAGGACAAATGTGGGAGAACCCCATTGCACTATGCAGCTGCAAATTGCCATTTCCACTGTATTAAAGCATTAGTGACCACCGGAGCCAACGTTAATGAAACAGACGACTGGGGACGGACAGCTCTGCACTACGCTGCTGCATCAGACATGGATAGAAATAAGATGATCTTAGGAAATGCCCATGACAATTCTGAAGAACTTGAAAGAGCCAGAGAAGTGAAGGAAAAAGATGCTGCACTAGTAAGGATGGCAAAAGTCCTCTGCACATGA
Peptide sequence:
MTAVHGRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARHGHELLINTLITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDTFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIKALVTTGANVNETDDWGRTALHYAAASDMDRNKMILGNAHDNSEELERAREVKEKDAALVRMAKVLCT
IRESfinder result
IRESfinder score
0.325703801
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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