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mmu_circAnkfy1_1128
Basic information of circular RNA
PanCircBase ID:
mmu_circAnkfy1_1128
mm39_Chr. Position ID:
chr11|72640125|72646716|+
Gene name:
Ankfy1
Transcript:
ENSMUST00000127610.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
12,13,14,15,16,17,18
Exon/intron offsets:
0,780,1101,2765,4300,5037,6390
Genomic length:
6591
Exon/intron sizes in nt:
2,29,99,15,41,69,11,91,00,000
Flanking exon/introns:
chr11:72637779-72640125|chr11:72646716-72648118
Splice length:
1128
Other database IDs:
circBase: hsa_circ_0041533, CIRCpedia: HSA_CIRCpedia_20803
Human Ortholog IDs:
Mature spliced Sequence:
GGAGAAACTCCTTTGCATACAGCTTGTCGGCATGGCCTGGCCAACCTCACAGCAGAGCTCCTGCAGCAAGGTGCCAATCCAAACTTACAGACAGAGGAGGCTCTGCCGGTACCTAAAGAGTCTCCCGTCCTAATGAGTTCTGCAGACAGCATCTACCTACAAACACCACTGCACATGGCAATTGCCTATAACCATCCAGATGTGGTTTCTGTCATCTTAGAGCAGAAAGCCAATGCTCTTCATGCCACCAACAACCTACAAATTATTCCAGACTTCAGCCTCAAGGATTCCAGAGACCAAACTGTTCTCGGCCTGGCATTATGGACTGGCATGCACACAATCGCTGCCCAGCTGCTGGGCTCCGGAGCCTCTATCAATGACACCATGTCAGATGGACAGACATTGCTGCACATGGCCATACAGCGGCAGGACAGCAAGAGCGCTCTCTTCCTACTGGAGCACCAAGCTGATATAAATGTCAGGACTCAGGACGGGGAGACTGCCCTCCAGCTGGCCATCAAACATCAGCTCCCACTAGTGGTTGATGCCATATGTACTCGAGGAGCTGACATGTCTGTGCCAGATGAAAAAGGGAACCCACCACTGTGGCTTGCCTTGGCAAGTAATCTGGAGGACATTGCTTCCACTCTGGTCAGACATGGTTGCGATGCTACATGCTGGGGCCCAGGACCCAGTGGGTGTCTTCAGACACTTCTGCATAGAGCCGTTGATGAAAACAATGAGTCTACTGCCTGCTTTCTTATTCGCAGTGGCTGTGACGTGAATAGTCCCAGACAACCAGGTACTAATGGAGAAGGAGAAGAGGAAGCAAGGGATGGACAGACTCCCCTGCATTTGGCAGCCTCATGGGGACTAGAAGAGACAGTACAGTGTCTTTTGGAGTTTGGTGCCAACGTCAATGCACAGGATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAGCGTCATCATTCAGCTGTTGATCTCTCACCCCAACATTGAGTTGAGCGTCCGGGACAGACAAGGGCTGACTCCTTTTGCCTGTGCCATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTGCTGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GACAGACAAGGGCTGACTCC
60.04
GAGCTCTGCTGTGAGGTTGG
60.67
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACAGACAAGGGCTGACTCC
60.04
GGAGCTCTGCTGTGAGGTTG
60.67
160
PCR template sequence:
GGACAGACAAGGGCTGACTCCTTTTGCCTGTGCCATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTGCTGAGCAGGGAGAAACTCCTTTGCATACAGCTTGTCGGCATGGCCTGGCCAACCTCACAGCAGAGCTCCTGCAGCAAGGTGCCAATCCAAACTTACAGACAGAGGAGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(2), mmu-miR-204-5p(2), mmu-miR-135a-5p(1), mmu-miR-33-5p(1), mmu-miR-128-3p(1), mmu-miR-136-5p(1), mmu-miR-676-3p(1), mmu-miR-183-5p(1), mmu-miR-362-5p(1), mmu-miR-705(1), mmu-miR-207(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCTGCTGAGCAGGGAGAAACTCCTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANKFY1_1128_ORF_1
1.0709
0.227696238
0.999997633
2124
ORF sequence:
ATGAGTTCTGCAGACAGCATCTACCTACAAACACCACTGCACATGGCAATTGCCTATAACCATCCAGATGTGGTTTCTGTCATCTTAGAGCAGAAAGCCAATGCTCTTCATGCCACCAACAACCTACAAATTATTCCAGACTTCAGCCTCAAGGATTCCAGAGACCAAACTGTTCTCGGCCTGGCATTATGGACTGGCATGCACACAATCGCTGCCCAGCTGCTGGGCTCCGGAGCCTCTATCAATGACACCATGTCAGATGGACAGACATTGCTGCACATGGCCATACAGCGGCAGGACAGCAAGAGCGCTCTCTTCCTACTGGAGCACCAAGCTGATATAAATGTCAGGACTCAGGACGGGGAGACTGCCCTCCAGCTGGCCATCAAACATCAGCTCCCACTAGTGGTTGATGCCATATGTACTCGAGGAGCTGACATGTCTGTGCCAGATGAAAAAGGGAACCCACCACTGTGGCTTGCCTTGGCAAGTAATCTGGAGGACATTGCTTCCACTCTGGTCAGACATGGTTGCGATGCTACATGCTGGGGCCCAGGACCCAGTGGGTGTCTTCAGACACTTCTGCATAGAGCCGTTGATGAAAACAATGAGTCTACTGCCTGCTTTCTTATTCGCAGTGGCTGTGACGTGAATAGTCCCAGACAACCAGGTACTAATGGAGAAGGAGAAGAGGAAGCAAGGGATGGACAGACTCCCCTGCATTTGGCAGCCTCATGGGGACTAGAAGAGACAGTACAGTGTCTTTTGGAGTTTGGTGCCAACGTCAATGCACAGGATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAGCGTCATCATTCAGCTGTTGATCTCTCACCCCAACATTGAGTTGAGCGTCCGGGACAGACAAGGGCTGACTCCTTTTGCCTGTGCCATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTGCTGAGCAGGGAGAAACTCCTTTGCATACAGCTTGTCGGCATGGCCTGGCCAACCTCACAGCAGAGCTCCTGCAGCAAGGTGCCAATCCAAACTTACAGACAGAGGAGGCTCTGCCGGTACCTAAAGAGTCTCCCGTCCTAATGAGTTCTGCAGACAGCATCTACCTACAAACACCACTGCACATGGCAATTGCCTATAACCATCCAGATGTGGTTTCTGTCATCTTAGAGCAGAAAGCCAATGCTCTTCATGCCACCAACAACCTACAAATTATTCCAGACTTCAGCCTCAAGGATTCCAGAGACCAAACTGTTCTCGGCCTGGCATTATGGACTGGCATGCACACAATCGCTGCCCAGCTGCTGGGCTCCGGAGCCTCTATCAATGACACCATGTCAGATGGACAGACATTGCTGCACATGGCCATACAGCGGCAGGACAGCAAGAGCGCTCTCTTCCTACTGGAGCACCAAGCTGATATAAATGTCAGGACTCAGGACGGGGAGACTGCCCTCCAGCTGGCCATCAAACATCAGCTCCCACTAGTGGTTGATGCCATATGTACTCGAGGAGCTGACATGTCTGTGCCAGATGAAAAAGGGAACCCACCACTGTGGCTTGCCTTGGCAAGTAATCTGGAGGACATTGCTTCCACTCTGGTCAGACATGGTTGCGATGCTACATGCTGGGGCCCAGGACCCAGTGGGTGTCTTCAGACACTTCTGCATAGAGCCGTTGATGAAAACAATGAGTCTACTGCCTGCTTTCTTATTCGCAGTGGCTGTGACGTGAATAGTCCCAGACAACCAGGTACTAATGGAGAAGGAGAAGAGGAAGCAAGGGATGGACAGACTCCCCTGCATTTGGCAGCCTCATGGGGACTAGAAGAGACAGTACAGTGTCTTTTGGAGTTTGGTGCCAACGTCAATGCACAGGATGCAGAAGGGAGAACGCCTGTCCATGTGGCCATCAGCAACCAGCATAGCGTCATCATTCAGCTGTTGATCTCTCACCCCAACATTGAGTTGAGCGTCCGGGACAGACAAGGGCTGACTCCTTTTGCCTGTGCCATGACTTACAAGAACAATAAGGCAGCTGAAGCCATTTTAAAACGAGAGTCTGGGGCTGCTGAGCAG
Peptide sequence:
MSSADSIYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGASINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIKHQLPLVVDAICTRGADMSVPDEKGNPPLWLALASNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAVDENNESTACFLIRSGCDVNSPRQPGTNGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPNIELSVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPVPKESPVLMSSADSIYLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGASINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIKHQLPLVVDAICTRGADMSVPDEKGNPPLWLALASNLEDIASTLVRHGCDATCWGPGPSGCLQTLLHRAVDENNESTACFLIRSGCDVNSPRQPGTNGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPVHVAISNQHSVIIQLLISHPNIELSVRDRQGLTPFACAMTYKNNKAAEAILKRESGAAEQ
IRESfinder result
IRESfinder score
0.414654918
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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