top of page
mmu_circAnk3_281
Basic information of circular RNA
PanCircBase ID:
mmu_circAnk3_281
mm39_Chr. Position ID:
chr10|69756267|69768326|+
Gene name:
Ank3
Transcript:
ENSMUST00000182884.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
24,25,26
Exon/intron offsets:
0,7257,11956
Genomic length:
12059
Exon/intron sizes in nt:
54,124,103
Flanking exon/introns:
chr10:69740340-69756267|chr10:69768326-69786781
Splice length:
281
Other database IDs:
CIRCpedia: MMU_CIRCpedia_27310, circAtlas: mmu-Ank3_0039
circBase: hsa_circ_0093811, circAtlas: hsa-ANK3_0027, CIRCpedia: HSA_CIRCpedia_119899
Human Ortholog IDs:
Mature spliced Sequence:
GTGATAAATGCACATGGTTCAAAATTCCCAAAGTACAGGAGGTTTTGGTGAAAAGTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACAGGACCTTAAGGAGCTAGGTGATGACTCCCTGCCAGCAGAAGGTTACGTAGGCTTCAGTCTTGGAGCCCGTTCTGCCAGCCTCCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGCTCCTACGCAAGGGAC
60.46
GTCAGTGTCCCCTGTGATGG
60.04
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGAAGCTCCTACGCAAGGG
60.46
GTCAGTGTCCCCTGTGATGG
60.04
147
PCR template sequence:
CCGCTCCTTCAGTTCGGATAGGTCCTACACCTTGAACAGAAGCTCCTACGCAAGGGACAGCATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAGGTGATAAATGCACATGGTTCAAAATTCCCAAAGTACAGGAGGTTTTGGTGAAAAGTGAAGATGCCATCACAGGGGACACTGACAAGTATCTCGGGCCACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-434-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCCAAAGAGCAGGTGATAAATGCAC
siRNA sets
TTTATCACCTGCTCTTTGG, TTATCACCTGCTCTTTGGA, ATTTATCACCTGCTCTTTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCANK3_281_ORF_1
0.9518
-0.101265696
0.037298169
90
ORF sequence:
ATGATGATAGAGGAACTTCTGGTACCATCCAAAGAGCAGGTGATAAATGCACATGGTTCAAAATTCCCAAAGTACAGGAGGTTTTGGTGA
Peptide sequence:
MMIEELLVPSKEQVINAHGSKFPKYRRFW
IRESfinder result
IRESfinder score
0.371492729
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page