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mmu_circAkt3_1514
Basic information of circular RNA
PanCircBase ID:
mmu_circAkt3_1514
mm39_Chr. Position ID:
chr1|176859215|177076447|-
Gene name:
Akt3
Transcript:
ENSMUST00000111159.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
13,12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,12125,18388,27684,35561,47124,48551,65317,71312,77977,99321,217026
Genomic length:
217232
Exon/intron sizes in nt:
103,88,215,129,123,69,66,132,145,112,126,206
Flanking exon/introns:
chr1:176853015-176859215|chr1:177076447-177085711
Splice length:
1514
Other database IDs:
CIRCpedia: MMU_CIRCpedia_12068
CIRCpedia: HSA_CIRCpedia_110913
Human Ortholog IDs:
Mature spliced Sequence:
GCTGAGTCATCACTAGAGAGTGGGAAGGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGAATCCAAACCCTAAAACTGACCTGCAAAGCACCATTTCTCCAAGTTGGGGGGCTCAGAGGGGAGCCATCATGAGCGATGTTACCATTGTGAAAGAAGGTTGGGTTCAGAAGAGGGGAGAATATATAAAAAACTGGAGGCCAAGATACTTCCTTTTGAAGACAGATGGCTCATTCATAGGCTATAAGGAGAAACCTCAAGATGTGGACTTACCTTATCCCCTCAACAACTTCTCAGTGGCAAAATGTCAGTTAATGAAAACAGAACGACCAAAGCCAAATACATTTATTATCAGATGTCTTCAGTGGACCACTGTTATAGAGAGAACATTTCATGTAGATACACCAGAGGAAAGAGAAGAGTGGACGGAAGCTATCCAAGCCGTAGCCGACCGATTGCAGAGGCAAGAGGAGGAGAGGATGAATTGTAGCCCAACCTCACAGATTGATAATATAGGAGAAGAAGAGATGGATGCGTCTACAACCCATCATAAAAGAAAGACGATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAGGATGAAGTGGCACACACTCTTACTGAAAGCAGAGTACTAAAGAACACCAGACATCCATTTTTAACATCCTTGAAATATTCCTTCCAGACAAAAGACCGTTTGTGTTTTGTGATGGAATATGTTAATGGCGGAGAGCTGTTTTTCCATTTGTCGAGAGAGCGAGTGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTCTGCTTTGGACTATCTACATTCTGGAAAGATTGTGTACCGTGATCTCAAGTTGGAGAATTTGATGCTAGATAAGGATGGCCATATAAAAATTACGGATTTTGGGCTTTGCAAAGAAGGGATCACAGATGCAGCTACCATGAAGACATTCTGTGGCACACCAGAGTACCTGGCACCAGAGGTATTAGAAGATAATGACTATGGCCGAGCCGTGGACTGGTGGGGCTTAGGTGTTGTCATGTATGAAATGATGTGTGGAAGGTTGCCTTTCTACAACCAGGATCATGAGAAACTCTTTGAATTAATACTAATGGAAGACATTAAATTCCCCCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGGCCAGATGATGCAAAAGAAATCATGAGGCATAGTTTTTTTTCTGGAGTAAACTGGCAAGATGTATATGACAAAAAGCTTGTACCTCCTTTTAAGCCTCAAGTAACATCTGAAACAGACACCCGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAGT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACATCTGAAACAGACACCCGA
59.31
GTTTGGATTCGCTGCTGCTG
60.46
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACATCTGAAACAGACACCCGA
59.9
GTTTGGATTCGCTGCTGCTG
60.46
177
PCR template sequence:
GTACCTCCTTTTAAGCCTCAAGTAACATCTGAAACAGACACCCGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAGTGCTGAGTCATCACTAGAGAGTGGGAAGGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCGAATCCAAAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-203-3p(1), mmu-miR-433-3p(1), mmu-miR-382-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-539-5p(1), mmu-miR-335-3p(1), mmu-miR-350-3p(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCACCTGAAAAGTGCTGAGTCATCAC
siRNA sets
ATGACTCAGCACTTTTCAG, GATGACTCAGCACTTTTCA, TGACTCAGCACTTTTCAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKT3_1514_ORF_1
0.8333
0.079545375
0.998287311
1359
ORF sequence:
ATGAGCGATGTTACCATTGTGAAAGAAGGTTGGGTTCAGAAGAGGGGAGAATATATAAAAAACTGGAGGCCAAGATACTTCCTTTTGAAGACAGATGGCTCATTCATAGGCTATAAGGAGAAACCTCAAGATGTGGACTTACCTTATCCCCTCAACAACTTCTCAGTGGCAAAATGTCAGTTAATGAAAACAGAACGACCAAAGCCAAATACATTTATTATCAGATGTCTTCAGTGGACCACTGTTATAGAGAGAACATTTCATGTAGATACACCAGAGGAAAGAGAAGAGTGGACGGAAGCTATCCAAGCCGTAGCCGACCGATTGCAGAGGCAAGAGGAGGAGAGGATGAATTGTAGCCCAACCTCACAGATTGATAATATAGGAGAAGAAGAGATGGATGCGTCTACAACCCATCATAAAAGAAAGACGATGAATGATTTTGACTATTTGAAACTACTAGGTAAAGGCACTTTTGGGAAAGTTATTTTGGTTCGAGAGAAGGCAAGTGGAAAATACTATGCTATGAAGATTCTGAAGAAAGAAGTCATTATTGCAAAGGATGAAGTGGCACACACTCTTACTGAAAGCAGAGTACTAAAGAACACCAGACATCCATTTTTAACATCCTTGAAATATTCCTTCCAGACAAAAGACCGTTTGTGTTTTGTGATGGAATATGTTAATGGCGGAGAGCTGTTTTTCCATTTGTCGAGAGAGCGAGTGTTCTCTGAGGACCGCACACGTTTCTATGGTGCAGAAATTGTCTCTGCTTTGGACTATCTACATTCTGGAAAGATTGTGTACCGTGATCTCAAGTTGGAGAATTTGATGCTAGATAAGGATGGCCATATAAAAATTACGGATTTTGGGCTTTGCAAAGAAGGGATCACAGATGCAGCTACCATGAAGACATTCTGTGGCACACCAGAGTACCTGGCACCAGAGGTATTAGAAGATAATGACTATGGCCGAGCCGTGGACTGGTGGGGCTTAGGTGTTGTCATGTATGAAATGATGTGTGGAAGGTTGCCTTTCTACAACCAGGATCATGAGAAACTCTTTGAATTAATACTAATGGAAGACATTAAATTCCCCCGAACACTCTCTTCAGATGCAAAATCATTGCTTTCAGGGCTCTTGATAAAGGATCCAAATAAACGCCTTGGTGGAGGGCCAGATGATGCAAAAGAAATCATGAGGCATAGTTTTTTTTCTGGAGTAAACTGGCAAGATGTATATGACAAAAAGCTTGTACCTCCTTTTAAGCCTCAAGTAACATCTGAAACAGACACCCGATATTTTGATGAAGAATTTACAGCTCAGACTATTACAATAACACCACCTGAAAAGTGCTGA
Peptide sequence:
MSDVTIVKEGWVQKRGEYIKNWRPRYFLLKTDGSFIGYKEKPQDVDLPYPLNNFSVAKCQLMKTERPKPNTFIIRCLQWTTVIERTFHVDTPEEREEWTEAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDEVAHTLTESRVLKNTRHPFLTSLKYSFQTKDRLCFVMEYVNGGELFFHLSRERVFSEDRTRFYGAEIVSALDYLHSGKIVYRDLKLENLMLDKDGHIKITDFGLCKEGITDAATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEDIKFPRTLSSDAKSLLSGLLIKDPNKRLGGGPDDAKEIMRHSFFSGVNWQDVYDKKLVPPFKPQVTSETDTRYFDEEFTAQTITITPPEKC
IRESfinder result
IRESfinder score
0.532954353
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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