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mmu_circAkap9_2388
Basic information of circular RNA
PanCircBase ID:
mmu_circAkap9_2388
mm39_Chr. Position ID:
chr5|4078373|4096941|+
Gene name:
Akap9
Transcript:
ENSMUST00000044492.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
22,23,24,25,26,27,28,29,30
Exon/intron offsets:
0,1466,4307,6374,10155,10865,15544,16425,17524
Genomic length:
18568
Exon/intron sizes in nt:
1,63,21,32,33,12,01,77,00,00,00,00,00,000
Flanking exon/introns:
chr5:4064079-4078373|chr5:4096941-4100154
Splice length:
2388
Other database IDs:
CIRCpedia: MMU_CIRCpedia_19297, circAtlas: mmu-Akap9_0013
Human Ortholog IDs:
Mature spliced Sequence:
CTTGAACATGCGAAAGTTACACAGACAGAGTTGATGCGTGAGTCCTTTAGACAAAAACAAGAGGCAACAGAGTCACTTCATTGCCTGGAAGAACTGCGAGAGCGCCTTCAGGAGGAGTCCAGGGCCAGGGAGCAGCTGGCCGAGGAGCTCAATAAAGCTGAGAGTGTCATTGATGGGTACTCGGATGAAAAAACTCTCTTTGAAAGGCAAATTCAAGAAAAAACCGATATTATAGAGCATCTTGAGCAGGAGGTGTTATGTATGAACAACAGACTGCAGGAGCTAGAGTCAGACCAACGCCGGGTGGAAGAGGAACGGCAGCTGCTGTGCAGGCAGAGGGAGGCGATGAGAGCAGAGGCTGGCCCCGTGGAGCAGCAATTTCTGCAGGAGACAGAAAAACTAATGAAGGAAAAGTTGGAAGTACAGTGTCAAGCTGAAAAAGTCCGTGGTGACCTTCAGAAGCAAGTGAAAGCTCTAGAAATAGATGTTGAAGAACAAGTCAGTAGGTTCATAGAACTGGAGCAAGAGAAAAACGCTGAACTCACAGATCTAAGGCAGCAAAGCCAAGCGCTGGAGAAGCAGCTGGAAAAAATGAGAAAATTTTTAGATGAGCAGGCCATCGATAGAGAGCACGAGAGAGATGTTTTTCAGCAGGAGATACAGAAGCTGGAGCATCAGCTTAAGGCTGTGCCTAGAATCCAGCCTGTGAGTGAGCACCAAGCCAGAGAGGTTGAACAGTTAACTAATCACTTGAAAGAGAAAACAGACAGGTGCAGTGAGCTGCTGCTATCCAAGGAGCAACTGCAGCGAGACATTCAGGAGAGGAATGAGGAGATTGAGAAGCTGGAGTGCCGAGTGCGAGAGCTGGAGCAAGCCCTGCTGGCTAGTGCAGAGCCCTTCCCCAAGGTAGAGGACCAGAAAAGGTCTGGAGCTGTAGAAGCAGACCCAGAATTATCCCTAGAAGTTCAGTTGCAGGTGGAGCGGGATGCTACAGACAGAAAACAGAAAGAGATCACCAACCTAGAAGAACAATTAGAACAGTTTCGGGAAGAACTGGAAAATAAGAACGATGAGGTCCAGGAGCTCCTCATGCAGCTGGAGATTCAGAGAAAAGAGTCCACCACCCGCCTGCAGGAGCTGCAGCAGGAAAACAGGCTATTTAAGGATGAAATAGAAAAACTTGGGTTTGCCATGAAGGAATCTGATTCTGTATCTACTCGGGATCAGCCCATGCTGTTTGGCAAATTTGCACAGCTAATACAAGAAAAGGAGATTGAAATTGACCGGTTAAATGAGCAGTTTATAAAACTGCAGCAGCAACTTAAACTCACAACGGATAACAAGGTCATTGAAGAACAAAAGGAACAGATACAAGATCTGGAAACCCAGATTGAACGTTTGATGAGTGAGCGAGAACATGAGAAGAAACAGAGAGAGGAAGAAGTAGAGCAGCTCACTGGGGTGGTTGAGAAACTGCAGCAGGAGGTGGTCAGCACTGAACAGCAGAGAGAGGGTGCCAGGACCCTCCCAGAAGATGAGGAGAGCTTTAAACATCAGCTGGACAAGGTGACTGCTGAAAAGCTGGTTTTAGAGCAACAAGTAGAAACCACCAACCAGGTAATGACGCACATGAACAATGTCCTTAAAGAAATCAATTTCAAAATGGATCAGATAACACAATCACTGTGCAACTTGAATAAAGAATGTGCCAGCAATGAAGAACTTCCAAGTCTACCTAAGGAAAGTGTCCACATGACTGTGCATGAGCTAGGCAGTGACAATCTGCAGCCAGAAGATGCCCCTGCCCAGGATGTGACCAAACCTCTAGAAAAACAGACCTCCCTTACGCGTCTTCAGAAAAGCCCTGAAGCTTCTAGAACACAAGAAATAGAGTCCCTGGCAAGCTCTGTTGGCGCCAAGGACGTTGAGCTTACCCAGTGTCGTGAACAAACAGAAACCATACAAGAGCAAGCCCAGTCTGAGACAGACAGGCTTCAGAAGAAGCTCACAGACCTGCAGAGAAGCCTTGAGAAGTTCGCCGCTGCACTGGTCAGTCAGGTCCAAATGGAGGCAGCTCAGGAGTATGTGCCGTTCCACCAAGAAAAGCAGCCGGTTTCATCAGCACCCGGAAGCACAGATATACAGAATGCAAATGGTCTGACTGGAGCCAGCACGGAGTCACTCATCCCCACAGTGACCCTGAGGCTAGCAGAGGTGGAGAGCCGTGTAGCTGAAGTGCACAGTGGTACAATGTCAGAAAAACTTGTGGGAATTGTGGGGGGAAATGCTTCAGAGACAGAAAAGAGAGTTATAGAGCTACAGAAGCTATTGGAGGAGGCCGAGGAAAGACCGGAAGAGGGAGGAGAACAAAGCTCTCGGGATGGGGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATTGGAGGAGGCCGAGGAA
60.03
TCGCAGTTCTTCCAGGCAAT
59.96
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGACCGGAAGAGGGAGGA
59.88
TCGCAGTTCTTCCAGGCAAT
59.96
143
PCR template sequence:
TTCAGAGACAGAAAAGAGAGTTATAGAGCTACAGAAGCTATTGGAGGAGGCCGAGGAAAGACCGGAAGAGGGAGGAGAACAAAGCTCTCGGGATGGGGAGCTTGAACATGCGAAAGTTACACAGACAGAGTTGATGCGTGAGTCCTTTAGACAAAAACAAGAGGCAACAGAGTCACTTCATTGCCTGGAAGAACTGCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
19
Interacting miRNAs (#binding sites)
mmu-miR-15a-5p(1), mmu-miR-19b-3p(1), mmu-miR-205-5p(1), mmu-miR-322-5p(1), mmu-miR-15b-5p(1), mmu-miR-19a-3p(1), mmu-miR-152-3p(1), mmu-miR-148b-3p(1), mmu-miR-148a-3p(1), mmu-miR-25-3p(1), mmu-miR-149-5p(1), mmu-miR-136-5p(1), mmu-miR-425-5p(1), mmu-miR-325-3p(1), mmu-miR-680(1), mmu-miR-29b-2-5p(1), mmu-miR-207(1), mmu-miR-335-3p(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
TCGGGATGGGGAGCTTGAACATGCGA
siRNA sets
ATGTTCAAGCTCCCCATCC,TTCAAGCTCCCCATCCCGA,GTTCAAGCTCCCCATCCCG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKAP9_2388_ORF_1
1.2431
0.334515548
1
4743
ORF sequence:
ATGCGTGAGTCCTTTAGACAAAAACAAGAGGCAACAGAGTCACTTCATTGCCTGGAAGAACTGCGAGAGCGCCTTCAGGAGGAGTCCAGGGCCAGGGAGCAGCTGGCCGAGGAGCTCAATAAAGCTGAGAGTGTCATTGATGGGTACTCGGATGAAAAAACTCTCTTTGAAAGGCAAATTCAAGAAAAAACCGATATTATAGAGCATCTTGAGCAGGAGGTGTTATGTATGAACAACAGACTGCAGGAGCTAGAGTCAGACCAACGCCGGGTGGAAGAGGAACGGCAGCTGCTGTGCAGGCAGAGGGAGGCGATGAGAGCAGAGGCTGGCCCCGTGGAGCAGCAATTTCTGCAGGAGACAGAAAAACTAATGAAGGAAAAGTTGGAAGTACAGTGTCAAGCTGAAAAAGTCCGTGGTGACCTTCAGAAGCAAGTGAAAGCTCTAGAAATAGATGTTGAAGAACAAGTCAGTAGGTTCATAGAACTGGAGCAAGAGAAAAACGCTGAACTCACAGATCTAAGGCAGCAAAGCCAAGCGCTGGAGAAGCAGCTGGAAAAAATGAGAAAATTTTTAGATGAGCAGGCCATCGATAGAGAGCACGAGAGAGATGTTTTTCAGCAGGAGATACAGAAGCTGGAGCATCAGCTTAAGGCTGTGCCTAGAATCCAGCCTGTGAGTGAGCACCAAGCCAGAGAGGTTGAACAGTTAACTAATCACTTGAAAGAGAAAACAGACAGGTGCAGTGAGCTGCTGCTATCCAAGGAGCAACTGCAGCGAGACATTCAGGAGAGGAATGAGGAGATTGAGAAGCTGGAGTGCCGAGTGCGAGAGCTGGAGCAAGCCCTGCTGGCTAGTGCAGAGCCCTTCCCCAAGGTAGAGGACCAGAAAAGGTCTGGAGCTGTAGAAGCAGACCCAGAATTATCCCTAGAAGTTCAGTTGCAGGTGGAGCGGGATGCTACAGACAGAAAACAGAAAGAGATCACCAACCTAGAAGAACAATTAGAACAGTTTCGGGAAGAACTGGAAAATAAGAACGATGAGGTCCAGGAGCTCCTCATGCAGCTGGAGATTCAGAGAAAAGAGTCCACCACCCGCCTGCAGGAGCTGCAGCAGGAAAACAGGCTATTTAAGGATGAAATAGAAAAACTTGGGTTTGCCATGAAGGAATCTGATTCTGTATCTACTCGGGATCAGCCCATGCTGTTTGGCAAATTTGCACAGCTAATACAAGAAAAGGAGATTGAAATTGACCGGTTAAATGAGCAGTTTATAAAACTGCAGCAGCAACTTAAACTCACAACGGATAACAAGGTCATTGAAGAACAAAAGGAACAGATACAAGATCTGGAAACCCAGATTGAACGTTTGATGAGTGAGCGAGAACATGAGAAGAAACAGAGAGAGGAAGAAGTAGAGCAGCTCACTGGGGTGGTTGAGAAACTGCAGCAGGAGGTGGTCAGCACTGAACAGCAGAGAGAGGGTGCCAGGACCCTCCCAGAAGATGAGGAGAGCTTTAAACATCAGCTGGACAAGGTGACTGCTGAAAAGCTGGTTTTAGAGCAACAAGTAGAAACCACCAACCAGGTAATGACGCACATGAACAATGTCCTTAAAGAAATCAATTTCAAAATGGATCAGATAACACAATCACTGTGCAACTTGAATAAAGAATGTGCCAGCAATGAAGAACTTCCAAGTCTACCTAAGGAAAGTGTCCACATGACTGTGCATGAGCTAGGCAGTGACAATCTGCAGCCAGAAGATGCCCCTGCCCAGGATGTGACCAAACCTCTAGAAAAACAGACCTCCCTTACGCGTCTTCAGAAAAGCCCTGAAGCTTCTAGAACACAAGAAATAGAGTCCCTGGCAAGCTCTGTTGGCGCCAAGGACGTTGAGCTTACCCAGTGTCGTGAACAAACAGAAACCATACAAGAGCAAGCCCAGTCTGAGACAGACAGGCTTCAGAAGAAGCTCACAGACCTGCAGAGAAGCCTTGAGAAGTTCGCCGCTGCACTGGTCAGTCAGGTCCAAATGGAGGCAGCTCAGGAGTATGTGCCGTTCCACCAAGAAAAGCAGCCGGTTTCATCAGCACCCGGAAGCACAGATATACAGAATGCAAATGGTCTGACTGGAGCCAGCACGGAGTCACTCATCCCCACAGTGACCCTGAGGCTAGCAGAGGTGGAGAGCCGTGTAGCTGAAGTGCACAGTGGTACAATGTCAGAAAAACTTGTGGGAATTGTGGGGGGAAATGCTTCAGAGACAGAAAAGAGAGTTATAGAGCTACAGAAGCTATTGGAGGAGGCCGAGGAAAGACCGGAAGAGGGAGGAGAACAAAGCTCTCGGGATGGGGAGCTTGAACATGCGAAAGTTACACAGACAGAGTTGATGCGTGAGTCCTTTAGACAAAAACAAGAGGCAACAGAGTCACTTCATTGCCTGGAAGAACTGCGAGAGCGCCTTCAGGAGGAGTCCAGGGCCAGGGAGCAGCTGGCCGAGGAGCTCAATAAAGCTGAGAGTGTCATTGATGGGTACTCGGATGAAAAAACTCTCTTTGAAAGGCAAATTCAAGAAAAAACCGATATTATAGAGCATCTTGAGCAGGAGGTGTTATGTATGAACAACAGACTGCAGGAGCTAGAGTCAGACCAACGCCGGGTGGAAGAGGAACGGCAGCTGCTGTGCAGGCAGAGGGAGGCGATGAGAGCAGAGGCTGGCCCCGTGGAGCAGCAATTTCTGCAGGAGACAGAAAAACTAATGAAGGAAAAGTTGGAAGTACAGTGTCAAGCTGAAAAAGTCCGTGGTGACCTTCAGAAGCAAGTGAAAGCTCTAGAAATAGATGTTGAAGAACAAGTCAGTAGGTTCATAGAACTGGAGCAAGAGAAAAACGCTGAACTCACAGATCTAAGGCAGCAAAGCCAAGCGCTGGAGAAGCAGCTGGAAAAAATGAGAAAATTTTTAGATGAGCAGGCCATCGATAGAGAGCACGAGAGAGATGTTTTTCAGCAGGAGATACAGAAGCTGGAGCATCAGCTTAAGGCTGTGCCTAGAATCCAGCCTGTGAGTGAGCACCAAGCCAGAGAGGTTGAACAGTTAACTAATCACTTGAAAGAGAAAACAGACAGGTGCAGTGAGCTGCTGCTATCCAAGGAGCAACTGCAGCGAGACATTCAGGAGAGGAATGAGGAGATTGAGAAGCTGGAGTGCCGAGTGCGAGAGCTGGAGCAAGCCCTGCTGGCTAGTGCAGAGCCCTTCCCCAAGGTAGAGGACCAGAAAAGGTCTGGAGCTGTAGAAGCAGACCCAGAATTATCCCTAGAAGTTCAGTTGCAGGTGGAGCGGGATGCTACAGACAGAAAACAGAAAGAGATCACCAACCTAGAAGAACAATTAGAACAGTTTCGGGAAGAACTGGAAAATAAGAACGATGAGGTCCAGGAGCTCCTCATGCAGCTGGAGATTCAGAGAAAAGAGTCCACCACCCGCCTGCAGGAGCTGCAGCAGGAAAACAGGCTATTTAAGGATGAAATAGAAAAACTTGGGTTTGCCATGAAGGAATCTGATTCTGTATCTACTCGGGATCAGCCCATGCTGTTTGGCAAATTTGCACAGCTAATACAAGAAAAGGAGATTGAAATTGACCGGTTAAATGAGCAGTTTATAAAACTGCAGCAGCAACTTAAACTCACAACGGATAACAAGGTCATTGAAGAACAAAAGGAACAGATACAAGATCTGGAAACCCAGATTGAACGTTTGATGAGTGAGCGAGAACATGAGAAGAAACAGAGAGAGGAAGAAGTAGAGCAGCTCACTGGGGTGGTTGAGAAACTGCAGCAGGAGGTGGTCAGCACTGAACAGCAGAGAGAGGGTGCCAGGACCCTCCCAGAAGATGAGGAGAGCTTTAAACATCAGCTGGACAAGGTGACTGCTGAAAAGCTGGTTTTAGAGCAACAAGTAGAAACCACCAACCAGGTAATGACGCACATGAACAATGTCCTTAAAGAAATCAATTTCAAAATGGATCAGATAACACAATCACTGTGCAACTTGAATAAAGAATGTGCCAGCAATGAAGAACTTCCAAGTCTACCTAAGGAAAGTGTCCACATGACTGTGCATGAGCTAGGCAGTGACAATCTGCAGCCAGAAGATGCCCCTGCCCAGGATGTGACCAAACCTCTAGAAAAACAGACCTCCCTTACGCGTCTTCAGAAAAGCCCTGAAGCTTCTAGAACACAAGAAATAGAGTCCCTGGCAAGCTCTGTTGGCGCCAAGGACGTTGAGCTTACCCAGTGTCGTGAACAAACAGAAACCATACAAGAGCAAGCCCAGTCTGAGACAGACAGGCTTCAGAAGAAGCTCACAGACCTGCAGAGAAGCCTTGAGAAGTTCGCCGCTGCACTGGTCAGTCAGGTCCAAATGGAGGCAGCTCAGGAGTATGTGCCGTTCCACCAAGAAAAGCAGCCGGTTTCATCAGCACCCGGAAGCACAGATATACAGAATGCAAATGGTCTGACTGGAGCCAGCACGGAGTCACTCATCCCCACAGTGACCCTGAGGCTAGCAGAGGTGGAGAGCCGTGTAGCTGAAGTGCACAGTGGTACAATGTCAGAAAAACTTGTGGGAATTGTGGGGGGAAATGCTTCAGAGACAGAAAAGAGAGTTATAGAGCTACAGAAGCTATTGGAGGAGGCCGAGGAAAGACCGGAAGAGGGAGGAGAACAAAGCTCTCGGGATGGGGAG
Peptide sequence:
MRESFRQKQEATESLHCLEELRERLQEESRAREQLAEELNKAESVIDGYSDEKTLFERQIQEKTDIIEHLEQEVLCMNNRLQELESDQRRVEEERQLLCRQREAMRAEAGPVEQQFLQETEKLMKEKLEVQCQAEKVRGDLQKQVKALEIDVEEQVSRFIELEQEKNAELTDLRQQSQALEKQLEKMRKFLDEQAIDREHERDVFQQEIQKLEHQLKAVPRIQPVSEHQAREVEQLTNHLKEKTDRCSELLLSKEQLQRDIQERNEEIEKLECRVRELEQALLASAEPFPKVEDQKRSGAVEADPELSLEVQLQVERDATDRKQKEITNLEEQLEQFREELENKNDEVQELLMQLEIQRKESTTRLQELQQENRLFKDEIEKLGFAMKESDSVSTRDQPMLFGKFAQLIQEKEIEIDRLNEQFIKLQQQLKLTTDNKVIEEQKEQIQDLETQIERLMSEREHEKKQREEEVEQLTGVVEKLQQEVVSTEQQREGARTLPEDEESFKHQLDKVTAEKLVLEQQVETTNQVMTHMNNVLKEINFKMDQITQSLCNLNKECASNEELPSLPKESVHMTVHELGSDNLQPEDAPAQDVTKPLEKQTSLTRLQKSPEASRTQEIESLASSVGAKDVELTQCREQTETIQEQAQSETDRLQKKLTDLQRSLEKFAAALVSQVQMEAAQEYVPFHQEKQPVSSAPGSTDIQNANGLTGASTESLIPTVTLRLAEVESRVAEVHSGTMSEKLVGIVGGNASETEKRVIELQKLLEEAEERPEEGGEQSSRDGELEHAKVTQTELMRESFRQKQEATESLHCLEELRERLQEESRAREQLAEELNKAESVIDGYSDEKTLFERQIQEKTDIIEHLEQEVLCMNNRLQELESDQRRVEEERQLLCRQREAMRAEAGPVEQQFLQETEKLMKEKLEVQCQAEKVRGDLQKQVKALEIDVEEQVSRFIELEQEKNAELTDLRQQSQALEKQLEKMRKFLDEQAIDREHERDVFQQEIQKLEHQLKAVPRIQPVSEHQAREVEQLTNHLKEKTDRCSELLLSKEQLQRDIQERNEEIEKLECRVRELEQALLASAEPFPKVEDQKRSGAVEADPELSLEVQLQVERDATDRKQKEITNLEEQLEQFREELENKNDEVQELLMQLEIQRKESTTRLQELQQENRLFKDEIEKLGFAMKESDSVSTRDQPMLFGKFAQLIQEKEIEIDRLNEQFIKLQQQLKLTTDNKVIEEQKEQIQDLETQIERLMSEREHEKKQREEEVEQLTGVVEKLQQEVVSTEQQREGARTLPEDEESFKHQLDKVTAEKLVLEQQVETTNQVMTHMNNVLKEINFKMDQITQSLCNLNKECASNEELPSLPKESVHMTVHELGSDNLQPEDAPAQDVTKPLEKQTSLTRLQKSPEASRTQEIESLASSVGAKDVELTQCREQTETIQEQAQSETDRLQKKLTDLQRSLEKFAAALVSQVQMEAAQEYVPFHQEKQPVSSAPGSTDIQNANGLTGASTESLIPTVTLRLAEVESRVAEVHSGTMSEKLVGIVGGNASETEKRVIELQKLLEEAEERPEEGGEQSSRDGE
IRESfinder result
IRESfinder score
0.452321687
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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