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mmu_circAkap10_798
Basic information of circular RNA
PanCircBase ID:
mmu_circAkap10_798
mm39_Chr. Position ID:
chr11|61768132|61791272|-
Gene name:
Akap10
Transcript:
ENSMUST00000102650.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
14,13,12,11,10,9,8
Exon/intron offsets:
0,667,9393,12931,16073,19341,23003
Genomic length:
23140
Exon/intron sizes in nt:
9,65,38,31,10,17,41,45,000
Flanking exon/introns:
chr11:61763847-61768132|chr11:61791272-61794732
Splice length:
798
Other database IDs:
circAtlas: mmu-Akap10_0029
Human Ortholog IDs:
Mature spliced Sequence:
TACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGTTTTCTGTCCAGCAATCTTTATTACAAATATTTGAATGATCTCATCCATTCAGTTCGAGGAGATGAATTTCTTGGAGGGAATGTTTCCCTGGCTGCTCACGGCTCTGTCTGCCTTCCTGAGGAGTCTCACTCAGGTGGTTCCGATGGCTCCACTGCTCAGTCTAGTGTGAAAAAAGCCAGTATTAAAATTCTGAAAAATTTTGATGAAGCAATAATTGTGGATGCTGCAAGTCTGGACCCAGAATCTTTATATCAACGGACATATGCAGGGAAGATGTCCTTTGGGAGAGTTAGTGATTTGGGGCAGTTCATCCGAGAGTCTGAGCCTGAACCTGATGTGAAGAAATCAAAAGGATTCATGTTCTCACAAGCTATGAAGAAGTGGGTGCAAGGAAATACTGACGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGGCCCAAGAAGAGCTAGC
60.18
TTTGGCAGCAAGCTGAGACT
60.18
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCCCAAGAAGAGCTAGCTT
59.75
TTTGGCAGCAAGCTGAGACT
60.18
178
PCR template sequence:
CGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAGTACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-328-3p(1), mmu-miR-214-3p(1), mmu-miR-24-3p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-484(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGTCTACAAAGTACATGGAAAAAG
siRNA sets
TTTTCCATGTACTTTGTAG,TTTCCATGTACTTTGTAGA,TTCCATGTACTTTGTAGAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAKAP10_798_ORF_1
1.0387
0.13319373
0.999832001
1593
ORF sequence:
ATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGTTTTCTGTCCAGCAATCTTTATTACAAATATTTGAATGATCTCATCCATTCAGTTCGAGGAGATGAATTTCTTGGAGGGAATGTTTCCCTGGCTGCTCACGGCTCTGTCTGCCTTCCTGAGGAGTCTCACTCAGGTGGTTCCGATGGCTCCACTGCTCAGTCTAGTGTGAAAAAAGCCAGTATTAAAATTCTGAAAAATTTTGATGAAGCAATAATTGTGGATGCTGCAAGTCTGGACCCAGAATCTTTATATCAACGGACATATGCAGGGAAGATGTCCTTTGGGAGAGTTAGTGATTTGGGGCAGTTCATCCGAGAGTCTGAGCCTGAACCTGATGTGAAGAAATCAAAAGGATTCATGTTCTCACAAGCTATGAAGAAGTGGGTGCAAGGAAATACTGACGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAGTACATGGAAAAAGAAGATGCAGTGAATATCTTACAATTCTGGTTAGCAGCGGATAATTTCCAGTCTCAGCTTGCTGCCAAAAAGGGCCAGTATGATGGACAGGAGGCCCAGAATGATGCCATGATTTTATATGACAAGTACTTTTCCCTCCAAGCCACACACCCCCTTGGATTTGATGATGTTGTACGATTAGAAATTGAATCTAATATCTGCAGGGAAGGTGGACCACTTCCTAATTGTTTCACAACTCCATTACGTCAGGCCTGGACAACCATGGAGAAGGTCTTTTTGCCTGGTTTTCTGTCCAGCAATCTTTATTACAAATATTTGAATGATCTCATCCATTCAGTTCGAGGAGATGAATTTCTTGGAGGGAATGTTTCCCTGGCTGCTCACGGCTCTGTCTGCCTTCCTGAGGAGTCTCACTCAGGTGGTTCCGATGGCTCCACTGCTCAGTCTAGTGTGAAAAAAGCCAGTATTAAAATTCTGAAAAATTTTGATGAAGCAATAATTGTGGATGCTGCAAGTCTGGACCCAGAATCTTTATATCAACGGACATATGCAGGGAAGATGTCCTTTGGGAGAGTTAGTGATTTGGGGCAGTTCATCCGAGAGTCTGAGCCTGAACCTGATGTGAAGAAATCAAAAGGATTCATGTTCTCACAAGCTATGAAGAAGTGGGTGCAAGGAAATACTGACGAGGCCCAAGAAGAGCTAGCTTGGAAGATTGCAAAAATGATAGTGAGTGATGTTATGCAGCAGGCACACCATGATCAACCACTAGAGAAGTCTACAAAG
Peptide sequence:
MEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLAAHGSVCLPEESHSGGSDGSTAQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMSFGRVSDLGQFIRESEPEPDVKKSKGFMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDVMQQAHHDQPLEKSTKYMEKEDAVNILQFWLAADNFQSQLAAKKGQYDGQEAQNDAMILYDKYFSLQATHPLGFDDVVRLEIESNICREGGPLPNCFTTPLRQAWTTMEKVFLPGFLSSNLYYKYLNDLIHSVRGDEFLGGNVSLAAHGSVCLPEESHSGGSDGSTAQSSVKKASIKILKNFDEAIIVDAASLDPESLYQRTYAGKMSFGRVSDLGQFIRESEPEPDVKKSKGFMFSQAMKKWVQGNTDEAQEELAWKIAKMIVSDVMQQAHHDQPLEKSTK
IRESfinder result
IRESfinder score
0.579408877
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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