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mmu_circAk7_396
Basic information of circular RNA
PanCircBase ID:
mmu_circAk7_396
mm39_Chr. Position ID:
chr12|105676403|105682338|+
Gene name:
Ak7
Transcript:
ENSMUST00000040876.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
2,3,4
Exon/intron offsets:
0,3358,5840
Genomic length:
5935
Exon/intron sizes in nt:
192,109,95
Flanking exon/introns:
chr12:105672525-105676403|chr12:105682338-105692445
Splice length:
396
Other database IDs:
CIRCpedia: MMU_CIRCpedia_1538, circAtlas: mmu-Ak7_0002
circAtlas: hsa-AK7_0002, CIRCpedia: HSA_CIRCpedia_370723
Human Ortholog IDs:
Mature spliced Sequence:
TTCCTAGCTAACTGTGTCGCTGGGGCTTCACTGGAAGAGATTGGAGAGGAGGAGGAGGAGGAGGACGAGACAAAGTCAGCTGTAGCTCCAGAAGCCCCCACTACAAAAGTGAAGGAAGGCACCTTCCAGATCGTGGGCACCTTGGTCAAGCCAGAGAGTGTCAAGCCAGACTTCGCTGTGGAGGCTTATAAGGGCATCTCCCGGGAAGACCTGCTCTCGCATCTCATGGAGTGCGATGCTGTCATCTATAACATCACAGAGAATGTGCAGCAAGTGGAGGAAGCCTTGTGGGCAGTCTCCGCATTGAATGAAGAAATCAGCCATTTTGAAAAGCGAAAGGTGTTCATTTTGTTGTCGACAGTGATGACATGGGCGAGATCCAAACCCCTGGACCCT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCATTTTGAAAAGCGAAAGGTG
60.06
TTGTCTCGTCCTCCTCCTCC
60.32
150
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCCATTTTGAAAAGCGAAAGG
59.82
TTGTCTCGTCCTCCTCCTCC
60.32
152
PCR template sequence:
CTCCGCATTGAATGAAGAAATCAGCCATTTTGAAAAGCGAAAGGTGTTCATTTTGTTGTCGACAGTGATGACATGGGCGAGATCCAAACCCCTGGACCCTTTCCTAGCTAACTGTGTCGCTGGGGCTTCACTGGAAGAGATTGGAGAGGAGGAGGAGGAGGAGGACGAGACAAAGTCAGCTGTAGCTCCAGAAGCCCCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-207(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
ACCCCTGGACCCTTTCCTAGCTAACT
siRNA sets
TTAGCTAGGAAAGGGTCCA, GTTAGCTAGGAAAGGGTCC, AGTTAGCTAGGAAAGGGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAK7_396_ORF_1
1.1426
0.397191048
0.929484575
567
ORF sequence:
ATGGAGTGCGATGCTGTCATCTATAACATCACAGAGAATGTGCAGCAAGTGGAGGAAGCCTTGTGGGCAGTCTCCGCATTGAATGAAGAAATCAGCCATTTTGAAAAGCGAAAGGTGTTCATTTTGTTGTCGACAGTGATGACATGGGCGAGATCCAAACCCCTGGACCCTTTCCTAGCTAACTGTGTCGCTGGGGCTTCACTGGAAGAGATTGGAGAGGAGGAGGAGGAGGAGGACGAGACAAAGTCAGCTGTAGCTCCAGAAGCCCCCACTACAAAAGTGAAGGAAGGCACCTTCCAGATCGTGGGCACCTTGGTCAAGCCAGAGAGTGTCAAGCCAGACTTCGCTGTGGAGGCTTATAAGGGCATCTCCCGGGAAGACCTGCTCTCGCATCTCATGGAGTGCGATGCTGTCATCTATAACATCACAGAGAATGTGCAGCAAGTGGAGGAAGCCTTGTGGGCAGTCTCCGCATTGAATGAAGAAATCAGCCATTTTGAAAAGCGAAAGGTGTTCATTTTGTTGTCGACAGTGATGACATGGGCGAGATCCAAACCCCTGGACCCT
Peptide sequence:
MECDAVIYNITENVQQVEEALWAVSALNEEISHFEKRKVFILLSTVMTWARSKPLDPFLANCVAGASLEEIGEEEEEEDETKSAVAPEAPTTKVKEGTFQIVGTLVKPESVKPDFAVEAYKGISREDLLSHLMECDAVIYNITENVQQVEEALWAVSALNEEISHFEKRKVFILLSTVMTWARSKPLDP
IRESfinder result
IRESfinder score
0.495101897
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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