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mmu_circAhi1_391
Basic information of circular RNA
PanCircBase ID:
mmu_circAhi1_391
mm39_Chr. Position ID:
chr10|20860203|20864583|+
Gene name:
Ahi1
Transcript:
ENSMUST00000105525.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
13,14,15
Exon/intron offsets:
0,2665,4239
Genomic length:
4380
Exon/intron sizes in nt:
119,131,141
Flanking exon/introns:
chr10:20857329-20860203|chr10:20864583-20876404
Splice length:
391
Other database IDs:
circAtlas: mmu-Ahi1_0012
circAtlas: hsa-AHI1_0166, CIRCpedia: HSA_CIRCpedia_146847
Human Ortholog IDs:
Mature spliced Sequence:
GAAATTAAGGAAACTGAATTCAGGGGAGTTCCAATAAGTTACCTGGAGGTTCATCCCAATGGAAAGCGTTTGCTAATCCACACCAAAGACAGTACTCTGAGGATTATGGACCTGCGGATATTGGCAGCCAGGAAATTTGTGGGTGCAGCAAATTACCGTGAGAAGATCCATAGCACCTTGACGCCGTGCGGGACTCTGCTCTTTTCTGGGAGTGAGGATGGGATAGTATACGTTTGGAACCCAGAGACAGGAGAACAAGTGGCAATGTACTCTGACCTGCCATTCAAGTCCACAATCCGAGACATCTCTTACCACCCCCTGGAGAATATGGTTGCCTTCTGTGCCTTTGGACAGAGCGAGCCGATTCTTCTTTATATTTATGATTTCCAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCTTACCACCCCCTGGAGA
59.96
GCAAACGCTTTCCATTGGGA
59.68
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCTTACCACCCCCTGGAG
59.96
GCAAACGCTTTCCATTGGGA
59.68
160
PCR template sequence:
ACAATCCGAGACATCTCTTACCACCCCCTGGAGAATATGGTTGCCTTCTGTGCCTTTGGACAGAGCGAGCCGATTCTTCTTTATATTTATGATTTCCAGGGAAATTAAGGAAACTGAATTCAGGGGAGTTCCAATAAGTTACCTGGAGGTTCATCCCAATGGAAAGCGTTTGCTAATCCACACCAAAGACAGTACTCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-540-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TATGATTTCCAGGGAAATTAAGGAAA
siRNA sets
TAATTTCCCTGGAAATCAT, TTAATTTCCCTGGAAATCA, AATTTCCCTGGAAATCATA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCAHI1_391_ORF_1
0.8786
0.179914907
0.502258134
402
ORF sequence:
ATGATTTCCAGGGAAATTAAGGAAACTGAATTCAGGGGAGTTCCAATAAGTTACCTGGAGGTTCATCCCAATGGAAAGCGTTTGCTAATCCACACCAAAGACAGTACTCTGAGGATTATGGACCTGCGGATATTGGCAGCCAGGAAATTTGTGGGTGCAGCAAATTACCGTGAGAAGATCCATAGCACCTTGACGCCGTGCGGGACTCTGCTCTTTTCTGGGAGTGAGGATGGGATAGTATACGTTTGGAACCCAGAGACAGGAGAACAAGTGGCAATGTACTCTGACCTGCCATTCAAGTCCACAATCCGAGACATCTCTTACCACCCCCTGGAGAATATGGTTGCCTTCTGTGCCTTTGGACAGAGCGAGCCGATTCTTCTTTATATTTATGATTTCCAG
Peptide sequence:
MISREIKETEFRGVPISYLEVHPNGKRLLIHTKDSTLRIMDLRILAARKFVGAANYREKIHSTLTPCGTLLFSGSEDGIVYVWNPETGEQVAMYSDLPFKSTIRDISYHPLENMVAFCAFGQSEPILLYIYDFQ
IRESfinder result
IRESfinder score
0.744317711
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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