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mmu_circAdgrl3_1201
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrl3_1201
mm39_Chr. Position ID:
chr5|81345412|81660631|+
Gene name:
Adgrl3
Transcript:
ENSMUST00000132375.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,112305,267629,312158,314418
Genomic length:
315219
Exon/intron sizes in nt:
66,20,91,10,15,801
Flanking exon/introns:
chr5:81215568-81345412|chr5:81660631-81708709
Splice length:
1201
Other database IDs:
CIRCpedia: MMU_CIRCpedia_223393
CIRCpedia: HSA_CIRCpedia_373847
Human Ortholog IDs:
Mature spliced Sequence:
AAATGATTAATTCAGAAAGGCAGAAGACAAAGCTATGGATTTTTAGCAGTCACAGAACAAATTAAGGTGTCTCAAGTGGGATATCAGTGTTTATTTTTGAGGAATTTTTCTTTCTGTTTGTGTTTGGTTTTTAATCATTGATATTGGAGTACTGAAGAGGAGCCAGAAGTCTATTGATTTGGTTTCACTTCAGACCAGCCATTCCTCCGAGAACACAGTCATGTGGCCACCACAGCTACTCATCCTCACGATGCTTTTAGCACCTGTAGTTCATGATGCAATAACAGAACCCAGTGTGCAGTGGTGGCAGGTCCTGATGTATTTCCAGACCCATGTCCGGGAACATATAAATACCTTGAAGTGCAGTATGAATGTGTCCCTTATAAAGTGGAACAAAAAGTTTTTCTTTGTCCTGGACTGCTAAAAGGAGTGTACCAGAGTGAACACTTGTTTGAATCTGACCACCAATCTGGGGCATGGTGCAAGGACCCTCTACAGGCTTCTGACAAGATTTACTATATGCCCTGGACTCCCTACAGAACTGACACCCTGACAGAGTATTCGTCCAAAGATGACTTCATTGCTGGAAGGCCAACAACTACCTACAAGCTCCCTCACAGAGTGGATGGCACTGGATTTGTAGTATACGATGGTGCCCTCTTCTTCAACAAGGAGCGGACAAGGAACATTGTAAAGTTTGATTTGAGGACTAGGATAAAGAGTGGAGAGGCAATCATAGCAAATGCTAATTACCATGACACCTCCCCATACCGATGGGGTGGCAAGTCCGACATAGACTTGGCAGTGGATGAAAATGGATTATGGGTAATCTATGCAACAGAACAGAACAATGGCAAGATTGTCATTAGCCAGTTGAACCCTTACACCCTACGGATTGAGGGGACATGGGACACTGCCTATGATAAAAGGTCAGCTTCCAATGCATTCATGATTTGTGGGATTCTGTATGTGGTCAAATCTGTATATGAGGATGATGACAACGAGGCCACCGGTAATAAGATTGACTACATTTACAACACTGACCAAAGCAAGGATAGCTTGGTGGATGTACCCTTTCCCAACTCTTACCAGTACATAGCAGCTGTGGATTACAATCCCAGGGACAATCTGCTCTATGTGTGGAATAACTACCACGTTGTGAAATACTCTTTGGACTTCGGGCCTCTGGATAGTAGATCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCAGGGACAATCTGCTCT
60.25
ACTGATATCCCACTTGAGACACC
59.55
174
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCAGGGACAATCTGCTCT
60.25
CTGATATCCCACTTGAGACACCT
59.29
173
PCR template sequence:
GCTGTGGATTACAATCCCAGGGACAATCTGCTCTATGTGTGGAATAACTACCACGTTGTGAAATACTCTTTGGACTTCGGGCCTCTGGATAGTAGATCAGAAATGATTAATTCAGAAAGGCAGAAGACAAAGCTATGGATTTTTAGCAGTCACAGAACAAATTAAGGTGTCTCAAGTGGGATATCAGTGTTTATTTTTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-134-5p(1), mmu-miR-34a-5p(1), mmu-miR-33-5p(1), mmu-miR-181b-5p(1), mmu-miR-149-5p(1), mmu-miR-1198-5p(1), mmu-miR-335-3p(1), mmu-miR-3470b(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GATAGTAGATCAGAAATGATTAATTC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRL3_1201_ORF_1
0.9374
0.215714798
0.937995396
747
ORF sequence:
ATGCCCTGGACTCCCTACAGAACTGACACCCTGACAGAGTATTCGTCCAAAGATGACTTCATTGCTGGAAGGCCAACAACTACCTACAAGCTCCCTCACAGAGTGGATGGCACTGGATTTGTAGTATACGATGGTGCCCTCTTCTTCAACAAGGAGCGGACAAGGAACATTGTAAAGTTTGATTTGAGGACTAGGATAAAGAGTGGAGAGGCAATCATAGCAAATGCTAATTACCATGACACCTCCCCATACCGATGGGGTGGCAAGTCCGACATAGACTTGGCAGTGGATGAAAATGGATTATGGGTAATCTATGCAACAGAACAGAACAATGGCAAGATTGTCATTAGCCAGTTGAACCCTTACACCCTACGGATTGAGGGGACATGGGACACTGCCTATGATAAAAGGTCAGCTTCCAATGCATTCATGATTTGTGGGATTCTGTATGTGGTCAAATCTGTATATGAGGATGATGACAACGAGGCCACCGGTAATAAGATTGACTACATTTACAACACTGACCAAAGCAAGGATAGCTTGGTGGATGTACCCTTTCCCAACTCTTACCAGTACATAGCAGCTGTGGATTACAATCCCAGGGACAATCTGCTCTATGTGTGGAATAACTACCACGTTGTGAAATACTCTTTGGACTTCGGGCCTCTGGATAGTAGATCAGAAATGATTAATTCAGAAAGGCAGAAGACAAAGCTATGGATTTTTAGCAGTCACAGAACAAATTAA
Peptide sequence:
MPWTPYRTDTLTEYSSKDDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFDLRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGKIVISQLNPYTLRIEGTWDTAYDKRSASNAFMICGILYVVKSVYEDDDNEATGNKIDYIYNTDQSKDSLVDVPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGPLDSRSEMINSERQKTKLWIFSSHRTN
IRESfinder result
IRESfinder score
0.687577206
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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