top of page
mmu_circAdgrf5_928
Basic information of circular RNA
PanCircBase ID:
mmu_circAdgrf5_928
mm39_Chr. Position ID:
chr17|43721392|43739557|+
Gene name:
Adgrf5
Transcript:
ENSMUST00000226087.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
3,4,5,6,7,8,9
Exon/intron offsets:
0,11996,13913,14212,15783,16437,17943
Genomic length:
18165
Exon/intron sizes in nt:
55,15,91,77,11,05,91,40,000
Flanking exon/introns:
chr17:43714934-43721392|chr17:43739557-43741394
Splice length:
928
Other database IDs:
circAtlas: mmu-Gpr116_0001
Human Ortholog IDs:
Mature spliced Sequence:
ATTCTCCAAGAACATGAGCTAGCCGGGGAAGAGCTACTGAGGCCAAAAAGAGCAGCTGCAGCGGGTGATCGCGTGGCGGAAGAATACATGGTGGATATTGAGATCAGCTTTGAAAATGTCTCCTTCCTGGAGTCCATCAGAGCTCACTTGAACAACCTCAGTTTTCCAATTCGAGGGACCGAAGCTGACATTTTGAACATAGCAATGACTACAGTCTGCACTCCTGCTGGAAATGACCTCTTGTGCTTCTGTGAGAAAGGCTACCAGTGGTCTGAGGAAAGGTGTCTCCACTCTCTCACTTGTCAAGACTATGACAGTGCCCTGCCAGGCGGCTACTGCAGTTGTCTGAAAGGACTTCCTCCCCAGGGACCTTTCTGCCAGCTCCCAGAAGCATTCATTACCTTAAAACTCAAAGTCAGACTGAACATAGGATTTCAAGAAGACCTAAAGAACACGTCTTCTGCCCTCTATAGGTCCTACAAGACTGATCTGGAAAGAGCGTTCCGAGCGGGTTACAGAACTTTACCAGGATTCAGATCGGTGACTGTGACACAGTTCACCAAGGGCAGCGTGGTTGTGAACTACGTAGTCCGGGTTACATCGGCACCACTGCCTGGATCTATTCATAAAGCCAATGAACAAGTCATACAGAACCTCAACCATACTTACAAAATGGACTACAACTCCTTCCAGGGAACCCCGAGCAATGAGACGAAATTCACTGTCATACCAGAATTCATCTTTGAGGGAGACAATGTGACTCTGGAGTGTGAAACTGAGTTCGTGACCTCCAACACGTCTTGGTACTATGGAGAAAAACGCTCTGACATCCAGAACAGCGACAAATACTCCATTCACACCACTGTCATCAACAATATAAGCCTGATCACCCGCCTTACCATTTACAACTTCACACAGCATGATGCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATAAGCCTGATCACCCGCC
59.31
CATGTATTCTTCCGCCACGC
59.7
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATAAGCCTGATCACCCGCCT
61.06
CATGTATTCTTCCGCCACGC
59.7
142
PCR template sequence:
ATCCAGAACAGCGACAAATACTCCATTCACACCACTGTCATCAACAATATAAGCCTGATCACCCGCCTTACCATTTACAACTTCACACAGCATGATGCGGATTCTCCAAGAACATGAGCTAGCCGGGGAAGAGCTACTGAGGCCAAAAAGAGCAGCTGCAGCGGGTGATCGCGTGGCGGAAGAATACATGGTGGATATTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-298-5p(1), mmu-miR-411-3p(1), mmu-miR-382-5p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CAGCATGATGCGGATTCTCCAAGAAC
siRNA sets
TTCTTGGAGAATCCGCATC,TGGAGAATCCGCATCATGC,TTGGAGAATCCGCATCATG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADGRF5_928_ORF_1
0.9251
0.248754625
0.985304204
936
ORF sequence:
ATGATGCGGATTCTCCAAGAACATGAGCTAGCCGGGGAAGAGCTACTGAGGCCAAAAAGAGCAGCTGCAGCGGGTGATCGCGTGGCGGAAGAATACATGGTGGATATTGAGATCAGCTTTGAAAATGTCTCCTTCCTGGAGTCCATCAGAGCTCACTTGAACAACCTCAGTTTTCCAATTCGAGGGACCGAAGCTGACATTTTGAACATAGCAATGACTACAGTCTGCACTCCTGCTGGAAATGACCTCTTGTGCTTCTGTGAGAAAGGCTACCAGTGGTCTGAGGAAAGGTGTCTCCACTCTCTCACTTGTCAAGACTATGACAGTGCCCTGCCAGGCGGCTACTGCAGTTGTCTGAAAGGACTTCCTCCCCAGGGACCTTTCTGCCAGCTCCCAGAAGCATTCATTACCTTAAAACTCAAAGTCAGACTGAACATAGGATTTCAAGAAGACCTAAAGAACACGTCTTCTGCCCTCTATAGGTCCTACAAGACTGATCTGGAAAGAGCGTTCCGAGCGGGTTACAGAACTTTACCAGGATTCAGATCGGTGACTGTGACACAGTTCACCAAGGGCAGCGTGGTTGTGAACTACGTAGTCCGGGTTACATCGGCACCACTGCCTGGATCTATTCATAAAGCCAATGAACAAGTCATACAGAACCTCAACCATACTTACAAAATGGACTACAACTCCTTCCAGGGAACCCCGAGCAATGAGACGAAATTCACTGTCATACCAGAATTCATCTTTGAGGGAGACAATGTGACTCTGGAGTGTGAAACTGAGTTCGTGACCTCCAACACGTCTTGGTACTATGGAGAAAAACGCTCTGACATCCAGAACAGCGACAAATACTCCATTCACACCACTGTCATCAACAATATAAGCCTGATCACCCGCCTTACCATTTACAACTTCACACAGCATGATGCG
Peptide sequence:
MMRILQEHELAGEELLRPKRAAAAGDRVAEEYMVDIEISFENVSFLESIRAHLNNLSFPIRGTEADILNIAMTTVCTPAGNDLLCFCEKGYQWSEERCLHSLTCQDYDSALPGGYCSCLKGLPPQGPFCQLPEAFITLKLKVRLNIGFQEDLKNTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVNYVVRVTSAPLPGSIHKANEQVIQNLNHTYKMDYNSFQGTPSNETKFTVIPEFIFEGDNVTLECETEFVTSNTSWYYGEKRSDIQNSDKYSIHTTVINNISLITRLTIYNFTQHDA
IRESfinder result
IRESfinder score
0.642902043
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page