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mmu_circAdgb_678

Basic information of circular RNA
PanCircBase ID:

mmu_circAdgb_678

mm39_Chr. Position ID:

chr10|10281028|10287632|-

Gene name:

Adgb

Transcript:

ENSMUST00000179956.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

6

Exon/intron indexes:

17,16,15,14,13,12

Exon/intron offsets:

0,673,1827,2157,5502,6429

Genomic length:

6604

Exon/intron sizes in nt:

64,179,39,130,91,175

Flanking exon/introns:

chr10:10278751-10281028|chr10:10287632-10298409

Splice length:

678

Other database IDs:

circAtlas: mmu-Adgb_0015

Human Ortholog IDs:
Mature spliced Sequence:

GCGAATTCTGCGGAGAAACTTAGGGAATATGGTCTTTCTCACATCTGTAGCCATCCTGTGCTGGTGACCAGAAGTAGGTCTTGCCCTCTTGTGTCACCCCCCAAACCTCCTCCCTTACCTGCCTGGAAACTCATTCGCCATAAAAAGGAAACAGTGATAACCGATGAAGCTCAAGATGCTGTCCCGAAGAAGCCTGAGCAGTTCCTTGAGATTTCAAGTCCATTTTTGAATTATAGAATGACTCCATTTACGATTCCAACAGAAACACATTTTGTCCAATCTGTCATTAAGAAAGGAACACCGCTGGGATCCAGTCTTCCTCCCCTCGTGGAAAATGATTTAGTTGCAAGTACCAGCCAAGGGGAAATGAGCATAGTAAATGGAAACCAGTCTCAGGGCAATATTGCATTGCAAATTACACTTGGAAAAGGTAAAGATGAACCTTCAGAGCCTGCATTGGCGGACTTCCACCAACTTGAGGCTACTAGTTTGGACAGGGATTTGATCAGCCTGACTACAGCGACACTGGACAAGTCACAGGAAGAGTTAGCAATAAACGAAGGGGTTGCCAAAGAGATATGGTTAGACTTCGAAGACTTTTGTGTGTGCTTTCACCATATATATATTTTCCACAAGCCACATTCTTATTGTCTTAATTTTCAAAAATCAGAATTCAAG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

ACTTTTGTGTGTGCTTTCACCA

59.44

TGACACAAGAGGGCAAGACC

59.89

179

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

ACTTTTGTGTGTGCTTTCACCA

59.44

GACACAAGAGGGCAAGACCT

59.6

178

PCR template sequence:

ATGGTTAGACTTCGAAGACTTTTGTGTGTGCTTTCACCATATATATATTTTCCACAAGCCACATTCTTATTGTCTTAATTTTCAAAAATCAGAATTCAAGGCGAATTCTGCGGAGAAACTTAGGGAATATGGTCTTTCTCACATCTGTAGCCATCCTGTGCTGGTGACCAGAAGTAGGTCTTGCCCTCTTGTGTCACCCC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

10

Interacting miRNAs (#binding sites)

mmu-miR-7b-5p(1), mmu-miR-216a-5p(1), mmu-miR-7a-5p(1), mmu-miR-125b-5p(1), mmu-miR-339-5p(1), mmu-miR-381-3p(1), mmu-miR-125a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1)

siRNAs for circular RNA silencing
Junction sequence

ATCAGAATTCAAGGCGAATTCTGCGG

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCADGB_678_ORF_1

0.8912

-0.014672282

0.987842673

1119

ORF sequence:

ATGACTCCATTTACGATTCCAACAGAAACACATTTTGTCCAATCTGTCATTAAGAAAGGAACACCGCTGGGATCCAGTCTTCCTCCCCTCGTGGAAAATGATTTAGTTGCAAGTACCAGCCAAGGGGAAATGAGCATAGTAAATGGAAACCAGTCTCAGGGCAATATTGCATTGCAAATTACACTTGGAAAAGGTAAAGATGAACCTTCAGAGCCTGCATTGGCGGACTTCCACCAACTTGAGGCTACTAGTTTGGACAGGGATTTGATCAGCCTGACTACAGCGACACTGGACAAGTCACAGGAAGAGTTAGCAATAAACGAAGGGGTTGCCAAAGAGATATGGTTAGACTTCGAAGACTTTTGTGTGTGCTTTCACCATATATATATTTTCCACAAGCCACATTCTTATTGTCTTAATTTTCAAAAATCAGAATTCAAGGCGAATTCTGCGGAGAAACTTAGGGAATATGGTCTTTCTCACATCTGTAGCCATCCTGTGCTGGTGACCAGAAGTAGGTCTTGCCCTCTTGTGTCACCCCCCAAACCTCCTCCCTTACCTGCCTGGAAACTCATTCGCCATAAAAAGGAAACAGTGATAACCGATGAAGCTCAAGATGCTGTCCCGAAGAAGCCTGAGCAGTTCCTTGAGATTTCAAGTCCATTTTTGAATTATAGAATGACTCCATTTACGATTCCAACAGAAACACATTTTGTCCAATCTGTCATTAAGAAAGGAACACCGCTGGGATCCAGTCTTCCTCCCCTCGTGGAAAATGATTTAGTTGCAAGTACCAGCCAAGGGGAAATGAGCATAGTAAATGGAAACCAGTCTCAGGGCAATATTGCATTGCAAATTACACTTGGAAAAGGTAAAGATGAACCTTCAGAGCCTGCATTGGCGGACTTCCACCAACTTGAGGCTACTAGTTTGGACAGGGATTTGATCAGCCTGACTACAGCGACACTGGACAAGTCACAGGAAGAGTTAGCAATAAACGAAGGGGTTGCCAAAGAGATATGGTTAGACTTCGAAGACTTTTGTGTGTGCTTTCACCATATATATATTTTCCACAAGCCACATTCTTATTGTCTTAATTTTCAAAAATCAGAATTCAAG

Peptide sequence:

MTPFTIPTETHFVQSVIKKGTPLGSSLPPLVENDLVASTSQGEMSIVNGNQSQGNIALQITLGKGKDEPSEPALADFHQLEATSLDRDLISLTTATLDKSQEELAINEGVAKEIWLDFEDFCVCFHHIYIFHKPHSYCLNFQKSEFKANSAEKLREYGLSHICSHPVLVTRSRSCPLVSPPKPPPLPAWKLIRHKKETVITDEAQDAVPKKPEQFLEISSPFLNYRMTPFTIPTETHFVQSVIKKGTPLGSSLPPLVENDLVASTSQGEMSIVNGNQSQGNIALQITLGKGKDEPSEPALADFHQLEATSLDRDLISLTTATLDKSQEELAINEGVAKEIWLDFEDFCVCFHHIYIFHKPHSYCLNFQKSEFK

IRESfinder result

IRESfinder score

0.719702309

IRES/non IRES

IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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