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mmu_circAdam22_1248
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam22_1248
mm39_Chr. Position ID:
chr5|8142339|8186905|-
Gene name:
Adam22
Transcript:
ENSMUST00000050166.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
14
Exon/intron indexes:
28,27,26,25,24,23,22,21,20,19,18,17,16,15
Exon/intron offsets:
0,391,14662,19555,24205,25249,28708,34987,37869,38145,40059,42172,42473,44466
Genomic length:
44566
Exon/intron sizes in nt:
6,71,01,18,91,11,51,69,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr5:8132269-8142339|chr5:8186905-8191718
Splice length:
1248
Other database IDs:
CIRCpedia: MMU_CIRCpedia_30676, circAtlas: mmu-Adam22_0010
circAtlas: hsa-ADAM22_0007, CIRCpedia: HSA_CIRCpedia_163068
Human Ortholog IDs:
Mature spliced Sequence:
CTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGTAACTCCTGGCAAGGT
59.96
CTTGTTGAACAGGCAGGCAC
59.97
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCGCAGTAACTCCTGGCAAG
60.04
CTTGTTGAACAGGCAGGCAC
59.97
178
PCR template sequence:
AAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGACTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-27b-3p(1), mmu-miR-27a-3p(1), mmu-miR-7b-5p(1), mmu-miR-18a-5p(1), mmu-miR-217-5p(1), mmu-miR-125b-5p(1), mmu-miR-339-5p(1), mmu-miR-433-3p(1), mmu-miR-363-3p(1), mmu-miR-125a-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-207(1), mmu-miR-5099(1), mmu-miR-3065-5p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTAACTCAACTGACTACTATCTCCCG
siRNA sets
GAGATAGTAGTCAGTTGAG,ATAGTAGTCAGTTGAGTTA,AGATAGTAGTCAGTTGAGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM22_1248_ORF_1
0.899
0.104311043
0.99999469
2127
ORF sequence:
ATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACTGACTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATATCATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTGAGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGAAGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAAAAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATATGTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCAGGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTAACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAGACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGGCAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACATTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGCCCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTGCTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGCAACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCACCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCTGGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCAGTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATCTAACTCAACT
Peptide sequence:
MDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKVTASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRTLNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVCNRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNMGGNKKKIRGKRFRPRSNSTDYYLPKKFTQCNVEEYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCKKCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKVTASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRTLNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVCNRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNMGGNKKKIRGKRFRPRSNST
IRESfinder result
IRESfinder score
0.581543453
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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