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mmu_circAdam17_1151
Basic information of circular RNA
PanCircBase ID:
mmu_circAdam17_1151
mm39_Chr. Position ID:
chr12|21390472|21411792|-
Gene name:
Adam17
Transcript:
ENSMUST00000101551.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,203,2392,5149,5545,6825,9313,11071,13411,21187
Genomic length:
21320
Exon/intron sizes in nt:
89,145,114,90,57,134,169,89,131,133
Flanking exon/introns:
chr12:21386786-21390472|chr12:21411792-21423336
Splice length:
1151
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCCTTAGCTAATATTCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTTAGAAACATTACTAACTTTTTCAGCTTTGAAAAGACATTTTAAATTATACTTGACATCAAGTACCGAACGCTTTTCACAAAACTTGAGAGTCGTGGTGGTGGACGGGAAAGAAGAAAGCGAGTACAGCGTGAAGTGGCAGAACTTCTTCAGTGGTCACGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCCCACATAGGAGATGATGATGTTACAGTGAGAATCAACACAGATGGGGCAGAATATAACGTAGAGCCACTTTGGAGGTTTGTCAATGATACTAAAGATAAACGAATGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTTAAATGCAGATAGTGAAGAGCTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTTCGTCGAGTGAAGAGACGAGCTGAACCTAACCCCTTGAAGAATACTTGTAAATTACTGGTGGTAGCAGATCATCGATTTTATAAATACATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAGGCTACATTTCAGGCACTCGGGACAGAGTGACGACCGACTGCTTTTTAGAGTTTTTGATAGAGCTAATTGACCGAGTTGATGACATATACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAGCAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTTTCCCAAACGAAGAGAAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGAAGAAGCATCTAAAGTCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTAGCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACATCTATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACAACCCAACTGTGAAGAAAAACA
59.72
TCCTTATGGAGTGCTGCTGA
58.43
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACAACCCAACTGTGAAGAAAAACA
59.72
TCCTTATGGAGTGCTGCTGAA
59.09
155
PCR template sequence:
TTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACATCTATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAAGAGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCCTTAGCTAATATTCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-204-5p(1), mmu-miR-26b-5p(1), mmu-miR-362-5p(1), mmu-miR-207(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TATTCTCACAAAGAGAAGCTTGATTC
siRNA sets
ATCAAGCTTCTCTTTGTGA, AATCAAGCTTCTCTTTGTG, GAATCAAGCTTCTCTTTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCADAM17_1151_ORF_1
0.812
0.086428246
0.914385989
804
ORF sequence:
ATGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTTAAATGCAGATAGTGAAGAGCTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTTCGTCGAGTGAAGAGACGAGCTGAACCTAACCCCTTGAAGAATACTTGTAAATTACTGGTGGTAGCAGATCATCGATTTTATAAATACATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAGGCTACATTTCAGGCACTCGGGACAGAGTGACGACCGACTGCTTTTTAGAGTTTTTGATAGAGCTAATTGACCGAGTTGATGACATATACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAGCAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTTTCCCAAACGAAGAGAAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGAAGAAGCATCTAAAGTCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTAGCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTGTGAAGAAAAACATCTATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAAGAGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCCTTAG
Peptide sequence:
MLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLGYISGTRDRVTTDCFLEFLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKRSLILCSQTTTSSP
IRESfinder result
IRESfinder score
0.684384341
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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