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mmu_circAcvr1_373
Basic information of circular RNA
PanCircBase ID:
mmu_circAcvr1_373
mm39_Chr. Position ID:
chr2|58406035|58427885|-
Gene name:
Acvr1
Transcript:
ENSMUST00000090935.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
3,2
Exon/intron offsets:
0,21754
Genomic length:
21850
Exon/intron sizes in nt:
277,96
Flanking exon/introns:
chr2:58397533-58406035|chr2:58427885-58456798
Splice length:
373
Other database IDs:
circBase: mmu_circ_0010033, CIRCpedia: MMU_CIRCpedia_24203, circAtlas: mmu-Acvr1_0013
Human Ortholog IDs:
Mature spliced Sequence:
GAACAAAGGAGCTGCCCCCGTGTCACCCAGCCCTTCAGTGGAAGTCTGGAAAAGGAACAGAGGTGATATTGCAGTGGATGAGCAGAGAGAAGCCGGCCTCTGGTGCTCTTGAGCTGGTCTGCCCATAGGGAGCCTGCTGGGAGAAGGTACAGCTTCCGGAAGACTCCTCCCGGAGCGCCTCTCCCATCCTCCTCTCCCTTGGAGCAGTCAGTACCTCTCTGCTGGAGGATCTGGGCTGGGTGTGCCGGGAGCTGGCTTTAACTGTAGCCCTGTCAGGCTTTCCCCGGACCTCGCGGAAGAGCGTCACCAGCCCCCACGGCTTTCCAACACATCACCTCTTTTCATGCCGTTTGGCACAGATCGAATCTACAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGCTTTCCAACACATCACC
59.76
CGGCTTCTCTCTGCTCATCC
60.25
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CGGCTTTCCAACACATCACC
59.76
CCGGCTTCTCTCTGCTCATC
60.25
153
PCR template sequence:
CAGGCTTTCCCCGGACCTCGCGGAAGAGCGTCACCAGCCCCCACGGCTTTCCAACACATCACCTCTTTTCATGCCGTTTGGCACAGATCGAATCTACAGGGAACAAAGGAGCTGCCCCCGTGTCACCCAGCCCTTCAGTGGAAGTCTGGAAAAGGAACAGAGGTGATATTGCAGTGGATGAGCAGAGAGAAGCCGGCCTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-139-5p(1), mmu-miR-214-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
TCGAATCTACAGGGAACAAAGGAGCT
siRNA sets
TTGTTCCCTGTAGATTCGA, TTTGTTCCCTGTAGATTCG, CTTTGTTCCCTGTAGATTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACVR1_373_ORF_1
0.6893
0.177176567
0.071653255
96
ORF sequence:
ATGCCGTTTGGCACAGATCGAATCTACAGGGAACAAAGGAGCTGCCCCCGTGTCACCCAGCCCTTCAGTGGAAGTCTGGAAAAGGAACAGAGGTGA
Peptide sequence:
MPFGTDRIYREQRSCPRVTQPFSGSLEKEQR
IRESfinder result
IRESfinder score
0.543915442
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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