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mmu_circAcvr1_1328

Basic information of circular RNA
PanCircBase ID:

mmu_circAcvr1_1328

mm39_Chr. Position ID:

chr2|58338325|58369940|-

Gene name:

Acvr1

Transcript:

ENSMUST00000112599.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

7

Exon/intron indexes:

9,8,7,6,5,4,3

Exon/intron offsets:

0,10571,14567,20200,25859,29291,31351

Genomic length:

31615

Exon/intron sizes in nt:

13,11,98,27,61,47,10,00,00,000

Flanking exon/introns:

chr2:58337685-58338325|chr2:58369940-58390495

Splice length:

1328

Other database IDs:

CIRCpedia: MMU_CIRCpedia_224780, circAtlas: mmu-Acvr1_0010

Human Ortholog IDs:
Mature spliced Sequence:

ATGAGAAGCCCAAGGTCAACCAGAAACTTTACATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCACTGTGAAGGCCAGCAGTGTTTTTCTTCTCTGAGCATCAACGATGGCTTCCACGTCTACCAGAAGGGCTGCTTTCAGGTTTATGAGCAGGGGAAGATGACGTGTAAGACCCCGCCGTCACCTGGCCAGGCTGTGGAGTGCTGCCAAGGGGACTGGTGTAACAGGAACATCACGGCCCAGCTGCCCACTAAAGGGAAGTCCTTCCCCGGAACACAGAATTTCCACCTGGAAGTTGGCCTTATCATCCTCTCGGTGGTGTTTGCAGTATGTCTTTTAGCTTGCATCCTTGGAGTTGCTCTCAGGAAGTTTAAGAGACGCAATCAAGAGCGCCTGAACCCCAGAGACGTGGAGTATGGTACCATTGAAGGGCTCATCACCACCAATGTGGGAGACAGCACTCTAGCGGAACTACTAGATCACTCGTGTACATCAGGAAGTGGCTCCGGTCTTCCTTTCCTGGTACAGAGAACGGTGGCTCGCCAGATAACCCTGTTGGAGTGTGTCGGGAAGGGCCGGTATGGAGAAGTATGGAGGGGCAGCTGGCAAGGCGAAAATGTCGCTGTGAAGATCTTCTCCTCCCGAGACGAGAAGTCATGGTTCAGGGAGACGGAATTGTACAACACTGTGATGTTGAGGCATGAAAATATCTTAGGTTTCATCGCTTCAGACATGACCTCCAGACACTCCAGTACCCAGCTGTGGCTCATCACACATTACCATGAAATGGGATCGTTGTATGACTACCTTCAGCTCACTACTCTGGATACGGTTAGCTGCCTTCGGATTGTACTGTCCATAGCCAGCGGCCTTGCCCATTTGCACATAGAGATATTTGGGACCCAAGGGAAGTCCGCCATTGCCCATCGAGATCTGAAGAGCAAAAACATCCTGGTGAAGAAGAATGGACAGTGCTGCATAGCAGATTTGGGCCTGGCAGTCATGCATTCCCAGAGCACAAACCAGCTTGATGTGGGAAACAACCCCCGTGTGGGGACCAAGCGCTACATGGCTCCGGAAGTGCTCGATGAAACCATCCAAGTGGATTGCTTTGATTCTTATAAGAGGGTCGATATTTGGGCCTTTGGCCTTGTTCTGTGGGAAGTGGCCAGGCGAATGGTGAGCAATGGTATAGTGGAAGATTACAAGCCACCATTCTATGATGTGGTTCCCAATGACCCAAGTTTTGAAGATATGAGGAAAGTTGTCTGTGTGGATCAACAGAGGCCAAACATACCTAACAGATGGTTCTCAGACCCG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GTGTGGATCAACAGAGGCCA

59.96

AAACACTGCTGGCCTTCACA

60.4

144

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

TGTGTGGATCAACAGAGGCC

59.96

AAACACTGCTGGCCTTCACA

60.4

145

PCR template sequence:

TGATGTGGTTCCCAATGACCCAAGTTTTGAAGATATGAGGAAAGTTGTCTGTGTGGATCAACAGAGGCCAAACATACCTAACAGATGGTTCTCAGACCCGATGAGAAGCCCAAGGTCAACCAGAAACTTTACATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCACTGTGAAGGCCAGCAGTGTTTTTCTTC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

17

Interacting miRNAs (#binding sites)

mmu-miR-27b-3p(2), mmu-miR-27a-3p(2), mmu-miR-377-3p(2), mmu-miR-188-3p(2), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-7b-5p(1), mmu-miR-103-3p(1), mmu-miR-30a-5p(1), mmu-miR-7a-5p(1), mmu-miR-30d-5p(1), mmu-miR-434-3p(1), mmu-miR-107-3p(1), mmu-miR-29b-2-5p(1), mmu-miR-342-3p(1), mmu-miR-207(1), mmu-miR-3105-3p(1)

siRNAs for circular RNA silencing
Junction sequence

GTTCTCAGACCCGATGAGAAGCCCAA

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCACVR1_1328_ORF_1

0.8918

0.202477906

0.999318927

1401

ORF sequence:

ATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCACTGTGAAGGCCAGCAGTGTTTTTCTTCTCTGAGCATCAACGATGGCTTCCACGTCTACCAGAAGGGCTGCTTTCAGGTTTATGAGCAGGGGAAGATGACGTGTAAGACCCCGCCGTCACCTGGCCAGGCTGTGGAGTGCTGCCAAGGGGACTGGTGTAACAGGAACATCACGGCCCAGCTGCCCACTAAAGGGAAGTCCTTCCCCGGAACACAGAATTTCCACCTGGAAGTTGGCCTTATCATCCTCTCGGTGGTGTTTGCAGTATGTCTTTTAGCTTGCATCCTTGGAGTTGCTCTCAGGAAGTTTAAGAGACGCAATCAAGAGCGCCTGAACCCCAGAGACGTGGAGTATGGTACCATTGAAGGGCTCATCACCACCAATGTGGGAGACAGCACTCTAGCGGAACTACTAGATCACTCGTGTACATCAGGAAGTGGCTCCGGTCTTCCTTTCCTGGTACAGAGAACGGTGGCTCGCCAGATAACCCTGTTGGAGTGTGTCGGGAAGGGCCGGTATGGAGAAGTATGGAGGGGCAGCTGGCAAGGCGAAAATGTCGCTGTGAAGATCTTCTCCTCCCGAGACGAGAAGTCATGGTTCAGGGAGACGGAATTGTACAACACTGTGATGTTGAGGCATGAAAATATCTTAGGTTTCATCGCTTCAGACATGACCTCCAGACACTCCAGTACCCAGCTGTGGCTCATCACACATTACCATGAAATGGGATCGTTGTATGACTACCTTCAGCTCACTACTCTGGATACGGTTAGCTGCCTTCGGATTGTACTGTCCATAGCCAGCGGCCTTGCCCATTTGCACATAGAGATATTTGGGACCCAAGGGAAGTCCGCCATTGCCCATCGAGATCTGAAGAGCAAAAACATCCTGGTGAAGAAGAATGGACAGTGCTGCATAGCAGATTTGGGCCTGGCAGTCATGCATTCCCAGAGCACAAACCAGCTTGATGTGGGAAACAACCCCCGTGTGGGGACCAAGCGCTACATGGCTCCGGAAGTGCTCGATGAAACCATCCAAGTGGATTGCTTTGATTCTTATAAGAGGGTCGATATTTGGGCCTTTGGCCTTGTTCTGTGGGAAGTGGCCAGGCGAATGGTGAGCAATGGTATAGTGGAAGATTACAAGCCACCATTCTATGATGTGGTTCCCAATGACCCAAGTTTTGAAGATATGAGGAAAGTTGTCTGTGTGGATCAACAGAGGCCAAACATACCTAACAGATGGTTCTCAGACCCGATGAGAAGCCCAAGGTCAACCAGAAACTTTACATGTGTGTGTGTGAGGGCCTCTCCTGCGGGAACGAGGACCACTGTGAAGGCCAGCAGTGTTTTTCTTCTCTGA

Peptide sequence:

MCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGCFQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNFHLEVGLIILSVVFAVCLLACILGVALRKFKRRNQERLNPRDVEYGTIEGLITTNVGDSTLAELLDHSCTSGSGSGLPFLVQRTVARQITLLECVGKGRYGEVWRGSWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGFIASDMTSRHSSTQLWLITHYHEMGSLYDYLQLTTLDTVSCLRIVLSIASGLAHLHIEIFGTQGKSAIAHRDLKSKNILVKKNGQCCIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSYKRVDIWAFGLVLWEVARRMVSNGIVEDYKPPFYDVVPNDPSFEDMRKVVCVDQQRPNIPNRWFSDPMRSPRSTRNFTCVCVRASPAGTRTTVKASSVFLL

IRESfinder result

IRESfinder score

0.430142828

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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