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mmu_circAcly_806
Basic information of circular RNA
PanCircBase ID:
mmu_circAcly_806
mm39_Chr. Position ID:
chr11|100383330|100389588|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
20,19,18,17,16,15
Exon/intron offsets:
0,1032,1379,3355,5680,6116
Genomic length:
6258
Exon/intron sizes in nt:
111,82,197,105,169,142
Flanking exon/introns:
chr11:100375553-100383330|chr11:100389588-100393985
Splice length:
806
Other database IDs:
circAtlas: mmu-Acly_0009
Human Ortholog IDs:
Mature spliced Sequence:
GAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAGCCTGGATGCTTTAAGATCGGGAATACTGGTGGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCCCAGGCAGCGTGGCCTACGTCTCACGTTCAGGAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGACCACAGATGGTGTCTATGAGGGCGTGGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCACGTGCTGCGCTACCAGGACACTCCAGGAGTCAAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGGAATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGTGGTCTGCTGGTGTAT
59.38
GTCAAAGTCCAGCATGCCCT
60.61
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCAGTGGTCTGCTGGTGTAT
59.38
AGTCAAAGTCCAGCATGCCC
60.61
141
PCR template sequence:
AGAGGAATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-339-5p(1), mmu-miR-149-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTCTCCTCCGAGGAAAGAGTGCCAC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACLY_806_ORF_1
1.0882
0.382385789
0.994093112
945
ORF sequence:
ATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGTTGGGGGCATTAAGCCTGGATGCTTTAAGATCGGGAATACTGGTGGAATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCCCAGGCAGCGTGGCCTACGTCTCACGTTCAGGAGGCATGTCTAATGAACTCAATAACATCATCTCTCGGACCACAGATGGTGTCTATGAGGGCGTGGCCATCGGCGGGGACAGGTACCCTGGGTCCACATTCATGGATCACGTGCTGCGCTACCAGGACACTCCAGGAGTCAAGATGATCGTAGTTCTTGGGGAGATAGGGGGCACAGAGGAATATAAGATCTGCCGGGGCATCAAGGAGGGCCGCCTCACCAAGCCAGTGGTCTGCTGGTGTATCGGGACCTGTGCCACCATGTTCTCCTCCGAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGA
Peptide sequence:
MQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPVVCWCIGTCATMFSSEERVPPSSADIPRPSCGACRPGLCRACWTLTTCVPETSPQWLPWSTLSLGITSRSFTGDTRKS
IRESfinder result
IRESfinder score
0.433619078
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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