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mmu_circAcly_692
Basic information of circular RNA
PanCircBase ID:
mmu_circAcly_692
mm39_Chr. Position ID:
chr11|100386685|100395835|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
17,16,15,14,13,12
Exon/intron offsets:
0,2325,2761,7300,8343,8995
Genomic length:
9150
Exon/intron sizes in nt:
105,169,142,30,91,155
Flanking exon/introns:
chr11:100384906-100386685|chr11:100395835-100403701
Splice length:
692
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGAAGGTATCCCTGAGGC
59.89
ACAATGGCCGTCATGTGAGT
59.96
145
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAGGTATCCCTGAGGCCCTC
60.4
ACAATGGCCGTCATGTGAGT
59.96
141
PCR template sequence:
CACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-149-5p(1), mmu-miR-34b-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-1198-5p(1), mmu-miR-669d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGGCCAGCTACGGGAAGACCACTGG
siRNA sets
TCTTCCCGTAGCTGGCCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACLY_692_ORF_1
1.2649
0.43434666
0.979672227
696
ORF sequence:
ATGACGGCCATTGTGGGCATGGCCCTGGGCCACCGGCCCATTCCCAACCAGCCACCCACAGCAGCTCACACTGCCAACTTCCTCCTTAATGCCAGCGGGAGCACATCGACTCCAGCACCCAGTAGGACAGCATCTTTTTCTGAGTCCCGAGCTGATGAAGTGGCACCTGCAAAGAAAGCCAAGCCAGCTATGCCCCAAGATTCAGTCCCAAGTCCAAGATCCCTGCAAGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAAACTCACATGA
Peptide sequence:
MTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSATLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATGRPLGSPSMSLAQKLT
IRESfinder result
IRESfinder score
0.379334928
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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