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mmu_circAcly_416

Basic information of circular RNA
PanCircBase ID:

mmu_circAcly_416

mm39_Chr. Position ID:

chr11|100386685|100389588|-

Gene name:

Acly

Transcript:

ENSMUST00000107389.8

CircRNA type:

Exonic circular (circ)RNA

Exon/Intron count:

3

Exon/intron indexes:

17,16,15

Exon/intron offsets:

0,2325,2761

Genomic length:

2903

Exon/intron sizes in nt:

105,169,142

Flanking exon/introns:

chr11:100384906-100386685|chr11:100389588-100393985

Splice length:

416

Other database IDs:

circAtlas: mmu-Acly_0006

​

Human Ortholog IDs:
Mature spliced Sequence:

GAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACG

Divergent primers for circular RNA PCR

Primer Set-1

Forward primer
Tm
Reverse primer
Tm
Product size

GCAGAAGGTATCCCTGAGGC

59.89

TCAAAGTCCAGCATGCCCTG

60.61

172

Primer Set-2

Forward primer
Tm
Reverse primer
Tm
Product size

GCAGAAGGTATCCCTGAGGC

59.89

GTCAAAGTCCAGCATGCCCT

60.61

173

PCR template sequence:

CACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTC

Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs

3

Interacting miRNAs (#binding sites)

mmu-miR-149-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-669d-5p(1)

siRNAs for circular RNA silencing
Junction sequence

TGGGCCAGCTACGGAAAGAGTGCCAC

siRNA sets

NA

Protein-coding potential of circular RNAs

CPAT analysis

CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length

CIRCACLY_416_ORF_1

1.1436

0.33263845

0.902841556

555

ORF sequence:

ATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGATCCCTGTCTTCAAGAACATGGCTGACGCCATGAAGAAGCACCCGGAGGTAGACGTGCTGATCAACTTTGCGTCTCTGCGGTCCGCTTACGACAGCACCATGGAGACCATGAACTATGCCCAGATCCGCACCATAGCCATCATAGCAGAAGGTATCCCTGAGGCCCTCACACGGAAGCTCATCAAGAAGGCCGACCAGAAGGGAGTGACCATCATTGGGCCAGCTACGGAAAGAGTGCCACCCTCTTCAGCCGACATACCAAGGCCATCGTGTGGGGCATGCAGACCCGGGCTGTGCAGGGCATGCTGGACTTTGACTACGTGTGTTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGATCACAAGCAGAAGTTTTACTGGGGACACAAGGAAATCCTGA

Peptide sequence:

MQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMKKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATERVPPSSADIPRPSCGACRPGLCRACWTLTTCVPETSPQWLPWSTLSLGITSRSFTGDTRKS

IRESfinder result

IRESfinder score

0.424971098

IRES/non IRES

non-IRES

riboCIRC information

riboCIRC ID

NA

NA

Evidence
Peptide sequence:

NA

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