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mmu_circAcly_1206
Basic information of circular RNA
PanCircBase ID:
mmu_circAcly_1206
mm39_Chr. Position ID:
chr11|100403701|100414447|-
Gene name:
Acly
Transcript:
ENSMUST00000107389.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,1400,2086,3026,3900,6213,6739,7415,9106,10564
Genomic length:
10746
Exon/intron sizes in nt:
11,86,21,37,11,91,31,80,00,00,00,00,000
Flanking exon/introns:
chr11:100395835-100403701|chr11:100414447-100418620
Splice length:
1206
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTAAACCAGGTCTCTCTGCAGCCATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCACCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGCCTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCACTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGAGGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTGGATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGGACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGGTCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTGAAGGAGCATGAGGTC
59.82
AGGAGTTCTTTGCCGGTCTG
59.96
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CCCTGAAGGAGCATGAGGTC
59.82
CAGGAGTTCTTTGCCGGTCT
59.96
152
PCR template sequence:
CGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGTAAACCAGGTCTCTCTGCAGCCATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-143-3p(1), mmu-miR-325-3p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
ATGGGAGAAGTTGGTAAACCAGGTCT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACLY_1206_ORF_1
1.2866
0.472815428
0.999999913
2388
ORF sequence:
ATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCACCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGCCTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCACTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGAGGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTGGATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGGACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGGTCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTTGGTAAACCAGGTCTCTCTGCAGCCATGTCAGCCAAGGCAATTTCAGAGCAGACCGGCAAAGAACTCCTGTACAAGTACATCTGCACCACCTCGGCCATCCAGAACCGGTTCAAGTACGCCCGGGTGACTCCCGACACAGACTGGGCCCATCTGCTGCAGGACCACCCGTGGCTGCTCAGCCAGAGCTTGGTTGTCAAGCCAGACCAGTTAATCAAACGTCGAGGAAAGCTTGGCCTCGTCGGGGTCAATCTCTCTCTGGATGGAGTCAAATCCTGGCTAAAACCTCGCCTGGGACATGAAGCCACTGTCGGCAAGGCCAAAGGCTTCCTCAAGAACTTTCTCATTGAACCCTTCGTCCCCCACAGTCAGGCGGAGGAGTTCTACGTGTGCATCTATGCTACCCGGGAAGGAGACTACGTCCTGTTCCACCATGAAGGGGGTGTGGATGTGGGCGATGTGGATGCCAAAGCCCAGAAGCTGCTTGTGGGTGTGGACGAAAAGCTGAATACCGAGGACATTAAGAGACACCTGTTGGTCCATGCACCTGAGGACAAGAAAGAAGTCCTGGCCAGCTTCATCTCTGGTCTATTCAATTTCTACGAGGATCTGTACTTCACCTACCTTGAGATCAACCCCCTTGTGGTGACCAAAGATGGTGTCTACATCCTTGACTTGGCGGCCAAGGTGGATGCCACAGCTGACTACATCTGTAAAGTCAAGTGGGGTGATATAGAGTTCCCTCCCCCCTTTGGGCGTGAGGCGTACCCCGAGGAAGCCTACATTGCAGACCTGGATGCCAAAAGTGGAGCAAGCTTGAAGCTGACCTTGCTGAACCCCAAGGGGCGGATCTGGACCATGGTTGCTGGGGGTGGCGCCTCCGTCGTGTACAGTGACACCATCTGTGATCTTGGAGGTGTCAATGAACTGGCGAATTACGGGGAATACTCGGGTGCCCCCAGTGAACAACAGACCTATGACTATGCCAAGACCATCCTCTCACTTATGACTCGAGAGAAGCACCCAGAAGGCAAGATCCTCATCATTGGAGGCAGCATTGCAAACTTCACCAATGTGGCCGCCACCTTCAAGGGCATTGTGAGAGCGATTCGAGATTACCAGGGTCCCCTGAAGGAGCATGAGGTCACCATCTTTGTCCGAAGAGGTGGCCCCAACTATCAAGAGGGATTACGAGTGATGGGAGAAGTT
Peptide sequence:
MSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLGLVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNTEDIKRHLLVHAPEDKKEVLASFISGLFNFYEDLYFTYLEINPLVVTKDGVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKPGLSAAMSAKAISEQTGKELLYKYICTTSAIQNRFKYARVTPDTDWAHLLQDHPWLLSQSLVVKPDQLIKRRGKLGLVGVNLSLDGVKSWLKPRLGHEATVGKAKGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNTEDIKRHLLVHAPEDKKEVLASFISGLFNFYEDLYFTYLEINPLVVTKDGVYILDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPEGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEV
IRESfinder result
IRESfinder score
0.417181942
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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