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mmu_circAcbd5_902
Basic information of circular RNA
PanCircBase ID:
mmu_circAcbd5_902
mm39_Chr. Position ID:
chr2|22970679|22989776|+
Gene name:
Acbd5
Transcript:
ENSMUST00000227663.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
6,7,8,9,10,11
Exon/intron offsets:
0,6817,8923,9808,13621,18897
Genomic length:
19097
Exon/intron sizes in nt:
33,13,51,98,10,72,29,200
Flanking exon/introns:
chr2:22970465-22970679|chr2:22989776-23000256
Splice length:
902
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TCCGATTGGAGAAAATCTCTAAGTACCTAGAAGATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGAAGAGTTAAAAGGAGCAGAACAGAGCGGCAGTGATGATAAGAAAACGCTGAAGAAATCAGCAGATAAGAATTTGGAAATCATTGTTACCAATGGCTATAAAGGTAGCTTTGTTCAGGATATACAGAGTGACATTCATACTGATTCTTCCCGGAGCACCAGAAGCAGTGAAGATGAAAAGCCTGGAGATGAGAGCTCTCAGCAGACAGGACACACCATTGTCTGTGCTCACCAAGATAGAAATGAAGATCCCAGTGAAGATGCTTCAGGAATACACCATTTAACAAGTGATTCAGATAGTGAAGTGTACTGTGACTCTATGGAGCAATTTGGACAAGAAGAGTATTATTTAGGTGGTGATCCTACTCAGCACTTGGAAAGTTCTGGTTTTTGTGAAGATGCTCAGCAATCTCCTGGAAATGGCAGCATTGGGAAAATGTGGATGGTAGCAGTCAAAGGAAAAGGCGAAGTGAAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGAGAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGAGGGAATAGAATACCACATTTGAGCGAAGGACCCAAGGGTCGGCAAGTAGGAAGTGGAGGTGATGGAGAACGCTGGGGCTCAGACCGGGGGTCCAGAGGCAGCCTCAATGAGCAGATCGCTCTTGTGCTCATTAGATTGCAGGAGGACATGCAGAATGTCCTTCAGAGACTTCATAAACTGGAAACACTAACTGCTTCGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGATCGCTCTTGTGCTCA
60.74
GCTTTGGCATTTGAAGAGGTCA
59.7
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAGATCGCTCTTGTGCTCA
60.74
CAGCTTTGGCATTTGAAGAGGT
59.7
163
PCR template sequence:
CCTCAATGAGCAGATCGCTCTTGTGCTCATTAGATTGCAGGAGGACATGCAGAATGTCCTTCAGAGACTTCATAAACTGGAAACACTAACTGCTTCGCAGTCCGATTGGAGAAAATCTCTAAGTACCTAGAAGATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGATAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-101a-3p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-3470b(1), mmu-miR-350-3p(1), mmu-miR-1904(1), mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AACTGCTTCGCAGTCCGATTGGAGAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACBD5_902_ORF_1
0.907
0.097119101
0.570522103
483
ORF sequence:
ATGGAGCAATTTGGACAAGAAGAGTATTATTTAGGTGGTGATCCTACTCAGCACTTGGAAAGTTCTGGTTTTTGTGAAGATGCTCAGCAATCTCCTGGAAATGGCAGCATTGGGAAAATGTGGATGGTAGCAGTCAAAGGAAAAGGCGAAGTGAAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGAGAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGAGGGAATAGAATACCACATTTGAGCGAAGGACCCAAGGGTCGGCAAGTAGGAAGTGGAGGTGATGGAGAACGCTGGGGCTCAGACCGGGGGTCCAGAGGCAGCCTCAATGAGCAGATCGCTCTTGTGCTCATTAGATTGCAGGAGGACATGCAGAATGTCCTTCAGAGACTTCATAAACTGGAAACACTAACTGCTTCGCAGTCCGATTGGAGAAAATCTCTAAGTACCTAG
Peptide sequence:
MEQFGQEEYYLGGDPTQHLESSGFCEDAQQSPGNGSIGKMWMVAVKGKGEVKHGGEDGRSSSGAPHRETRGGESEDFSSVRRGRGNRIPHLSEGPKGRQVGSGGDGERWGSDRGSRGSLNEQIALVLIRLQEDMQNVLQRLHKLETLTASQSDWRKSLST
IRESfinder result
IRESfinder score
0.438571537
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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