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mmu_circAcad10_805
Basic information of circular RNA
PanCircBase ID:
mmu_circAcad10_805
mm39_Chr. Position ID:
chr5|121783431|121790167|-
Gene name:
Acad10
Transcript:
ENSMUST00000031412.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
7,6,5,4,3
Exon/intron offsets:
0,1333,2466,3985,6587
Genomic length:
6736
Exon/intron sizes in nt:
14,21,60,15,91,95,149
Flanking exon/introns:
chr5:121780743-121783431|chr5:121790167-121794076
Splice length:
805
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCTGGGAGGTGCAGAATCATGTCCCTTCTGGAACTATAGTGAAGGCTTTCATCAGAGGTGGCGACAGTGGGCCCTGGATAAGGTTTATAAAAGGAGAGATAACGACCGAGCACTTCTTGGAAGAATTCGGGAGACTGTGCTCTGAAATCGCAAAGACTTCCGTGCCCGTGAGCTCCTATTTCTCTCTGCTGACCAGTGAGCAGGTGACAAAGCAGTTCCCGGTGATGACTCAGGCCATCTCTCAGATCAGAGCAAAAGGCCTCCAGACTGCAGTCCTCACCAACAACTTTCATCTTTCCAGCGGGGAAAGCTTCCTGCCCCTGGACCGGAAACAGTTTGATGTGGTGGTTGAGTCCTGCCTGGAAGGCATCTGTAAGCCAGATCCCAGGATATTCCAGCTGTGCCTGCAGAGGCTGAGCCTGCAGCCCTCAGAGGCTATTTTTCTTGATGATCTGGGATCCAATCTAAAAGTGGCTGCCAGCCTGGGCATTCACACCATCAAGGTTGACAGGCCCGAGACAGCAGTGAAGGAGCTGGAGGCTCTACTGGGTTTCCCACTGCACTTGGGCGTTCCCAACACCCGGCCTGTGAGGAAGACCATGGCGATTCCACAAGATGCCTTGGAGAAGTACCTGAAGGGCTTGCTGGGGACCCACTCCACCGGCCCCATGGAGCTCCTTCAGTTTGATCATGGACAGTCAAACCCAACGTACTACATCAGGCTGGCTGACCGTCAGCTGGTCCTGAGGAAGAAACCCTCGGGGACCCTCCTCCCCTCTGCCCATGCCATAGAAAGGGAATTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTACATCAGGCTGGCTGACC
60.18
GCCACCTCTGATGAAAGCCT
60.03
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCAGCTGGTCCTGAGGAAGA
59.88
CTTATCCAGGGCCCACTGTC
59.82
154
PCR template sequence:
CAACGTACTACATCAGGCTGGCTGACCGTCAGCTGGTCCTGAGGAAGAAACCCTCGGGGACCCTCCTCCCCTCTGCCCATGCCATAGAAAGGGAATTCAGGCTGGGAGGTGCAGAATCATGTCCCTTCTGGAACTATAGTGAAGGCTTTCATCAGAGGTGGCGACAGTGGGCCCTGGATAAGGTTTATAAAAGGAGAGAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-204-5p(1), mmu-miR-298-5p(1), mmu-miR-24-3p(1), mmu-miR-382-5p(1), mmu-miR-666-5p(1), mmu-miR-680(1), mmu-miR-207(1), mmu-miR-532-5p(1), mmu-miR-484(1), mmu-miR-452-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AAAGGGAATTCAGGCTGGGAGGTGCA
siRNA sets
CCTCCCAGCCTGAATTCCC, CTCCCAGCCTGAATTCCCT, ACCTCCCAGCCTGAATTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCACAD10_805_ORF_1
1.1338
0.282594036
0.961660705
726
ORF sequence:
ATGACTCAGGCCATCTCTCAGATCAGAGCAAAAGGCCTCCAGACTGCAGTCCTCACCAACAACTTTCATCTTTCCAGCGGGGAAAGCTTCCTGCCCCTGGACCGGAAACAGTTTGATGTGGTGGTTGAGTCCTGCCTGGAAGGCATCTGTAAGCCAGATCCCAGGATATTCCAGCTGTGCCTGCAGAGGCTGAGCCTGCAGCCCTCAGAGGCTATTTTTCTTGATGATCTGGGATCCAATCTAAAAGTGGCTGCCAGCCTGGGCATTCACACCATCAAGGTTGACAGGCCCGAGACAGCAGTGAAGGAGCTGGAGGCTCTACTGGGTTTCCCACTGCACTTGGGCGTTCCCAACACCCGGCCTGTGAGGAAGACCATGGCGATTCCACAAGATGCCTTGGAGAAGTACCTGAAGGGCTTGCTGGGGACCCACTCCACCGGCCCCATGGAGCTCCTTCAGTTTGATCATGGACAGTCAAACCCAACGTACTACATCAGGCTGGCTGACCGTCAGCTGGTCCTGAGGAAGAAACCCTCGGGGACCCTCCTCCCCTCTGCCCATGCCATAGAAAGGGAATTCAGGCTGGGAGGTGCAGAATCATGTCCCTTCTGGAACTATAGTGAAGGCTTTCATCAGAGGTGGCGACAGTGGGCCCTGGATAAGGTTTATAAAAGGAGAGATAACGACCGAGCACTTCTTGGAAGAATTCGGGAGACTGTGCTCTGA
Peptide sequence:
MTQAISQIRAKGLQTAVLTNNFHLSSGESFLPLDRKQFDVVVESCLEGICKPDPRIFQLCLQRLSLQPSEAIFLDDLGSNLKVAASLGIHTIKVDRPETAVKELEALLGFPLHLGVPNTRPVRKTMAIPQDALEKYLKGLLGTHSTGPMELLQFDHGQSNPTYYIRLADRQLVLRKKPSGTLLPSAHAIEREFRLGGAESCPFWNYSEGFHQRWRQWALDKVYKRRDNDRALLGRIRETVL
IRESfinder result
IRESfinder score
0.513932671
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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